Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses
The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their functio...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2017/8283075 |
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| author | Changquan Wang Zhenmin Zhong Peng Sun Hanbing Zhong Hongzhe Li Fangyi Chen |
| author_facet | Changquan Wang Zhenmin Zhong Peng Sun Hanbing Zhong Hongzhe Li Fangyi Chen |
| author_sort | Changquan Wang |
| collection | DOAJ |
| description | The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their function. Startle response is a behavior closely related to the auditory function of hair cells; therefore it can be used to measure the function of hair cells. In this study, we developed a device to measure the startle response of zebrafish larvae. By applying various levels of stimulus, it showed that the system can discern a 10 dB difference. The hair cell in zebrafish can regenerate after damage due to noise exposure or drug treatment. With this device, we measured the startle response of zebrafish larvae during and after drug treatment. The results show a similar trend to the classical hair cell staining method. The startle response was reduced with drug treatment and recovered after removal of the drug. Together it demonstrated the capability of this behavioral assay in evaluating the hair cell functions of fish larvae and its potential as a high-throughput screening tool for auditory-related gene and drug discovery. |
| format | Article |
| id | doaj-art-51635ff12e3f45d7a2e40d3924cca91e |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-51635ff12e3f45d7a2e40d3924cca91e2025-02-03T01:09:08ZengWileyNeural Plasticity2090-59041687-54432017-01-01201710.1155/2017/82830758283075Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle ResponsesChangquan Wang0Zhenmin Zhong1Peng Sun2Hanbing Zhong3Hongzhe Li4Fangyi Chen5Department of Biomedical Engineering, South University of Science and Technology of China, Guangdong, ChinaDepartment of Biomedical Engineering, South University of Science and Technology of China, Guangdong, ChinaDepartment of Biomedical Engineering, South University of Science and Technology of China, Guangdong, ChinaDepartment of Biomedical Engineering, South University of Science and Technology of China, Guangdong, ChinaResearch Service, VA Loma Linda Healthcare System, Loma Linda, CA 92357, USADepartment of Biomedical Engineering, South University of Science and Technology of China, Guangdong, ChinaThe zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their function. Startle response is a behavior closely related to the auditory function of hair cells; therefore it can be used to measure the function of hair cells. In this study, we developed a device to measure the startle response of zebrafish larvae. By applying various levels of stimulus, it showed that the system can discern a 10 dB difference. The hair cell in zebrafish can regenerate after damage due to noise exposure or drug treatment. With this device, we measured the startle response of zebrafish larvae during and after drug treatment. The results show a similar trend to the classical hair cell staining method. The startle response was reduced with drug treatment and recovered after removal of the drug. Together it demonstrated the capability of this behavioral assay in evaluating the hair cell functions of fish larvae and its potential as a high-throughput screening tool for auditory-related gene and drug discovery.http://dx.doi.org/10.1155/2017/8283075 |
| spellingShingle | Changquan Wang Zhenmin Zhong Peng Sun Hanbing Zhong Hongzhe Li Fangyi Chen Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses Neural Plasticity |
| title | Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses |
| title_full | Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses |
| title_fullStr | Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses |
| title_full_unstemmed | Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses |
| title_short | Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses |
| title_sort | evaluation of the hair cell regeneration in zebrafish larvae by measuring and quantifying the startle responses |
| url | http://dx.doi.org/10.1155/2017/8283075 |
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