Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities
Essential oils (EOs) have unique properties, such as antibacterial, antioxidant, and antiviral activities, which are beneficial in various industries, including cosmetics, food, and pharmaceuticals. In this study, the antioxidant and antimicrobial activities of <i>Pentacalia vaccinioides</i...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/12/1/9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587494271483904 |
|---|---|
| author | Luis G. Sequeda-Castañeda María A. Castellanos-Gómez Carlos L. A. Céspedes-Acuña |
| author_facet | Luis G. Sequeda-Castañeda María A. Castellanos-Gómez Carlos L. A. Céspedes-Acuña |
| author_sort | Luis G. Sequeda-Castañeda |
| collection | DOAJ |
| description | Essential oils (EOs) have unique properties, such as antibacterial, antioxidant, and antiviral activities, which are beneficial in various industries, including cosmetics, food, and pharmaceuticals. In this study, the antioxidant and antimicrobial activities of <i>Pentacalia vaccinioides</i> EOs obtained from leaves and flowers (fresh and dried plant material) were evaluated using hydrodistillation (HD), steam distillation (SD), simultaneous distillation–extraction (SDE), and solid-phase microextraction (SPME) techniques. Antimicrobial activity (minimum inhibitory concentration, MIC) and antioxidant capacity (half-maximal inhibitory concentration, IC<sub>50</sub>) were determined. The identification and quantification of the compounds present in the EOs were conducted by gas chromatography coupled to mass spectrometry (GC-MS). The main secondary metabolites identified in most samples obtained by different extraction techniques included phenol (~18%), 1S-α-pinene (~15%), β-phellandrene (~13%), β-pinene (~12%), 4-terpineol (~10%), γ-terpinene (~10%), trans-nerolidol (~8%), limonene (~8%), and β-thujene (~6%). EOs obtained by HD, SD, and SDE exhibited antioxidant activity, with IC<sub>50</sub> values between 621.7 and 696.6 µg/mL. Additionally, the EOs demonstrated bactericidal activity against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, with MIC values of 5.0 and 45 µg/mL, respectively. <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> did not show antimicrobial susceptibility to EOs. This study constitutes the first evaluation of <i>Pentacalia vaccinioides</i> EOs, demonstrating their bioactive potential and the relevance of the extraction method. The findings highlight this species as a promising source of natural compounds for therapeutic and preservative applications, depending on the type of plant material and extraction technique used. Future research should investigate how microclimatic conditions and plant development affect the chemical composition and elucidate the molecular mechanisms behind the observed bioactivities to better understand their cellular actions. Furthermore, the evaluation of the applications of EOs and hydrolates in the pharmaceutical and food industries, along with the exploration of the bioactive potential of extraction-derived hydrolates, offers a promising avenue to maximize plant utility. |
| format | Article |
| id | doaj-art-514c37e6e7844e15b84088bc50068f1b |
| institution | Kabale University |
| issn | 2297-8739 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj-art-514c37e6e7844e15b84088bc50068f1b2025-01-24T13:49:31ZengMDPI AGSeparations2297-87392025-01-01121910.3390/separations12010009Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant ActivitiesLuis G. Sequeda-Castañeda0María A. Castellanos-Gómez1Carlos L. A. Céspedes-Acuña2Chemistry Department, Sciences Faculty, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaChemistry Department, Sciences Faculty, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaBasic Sciences Department, Sciences Faculty, Universidad del Bio Bio, Chillán 3800708, ChileEssential oils (EOs) have unique properties, such as antibacterial, antioxidant, and antiviral activities, which are beneficial in various industries, including cosmetics, food, and pharmaceuticals. In this study, the antioxidant and antimicrobial activities of <i>Pentacalia vaccinioides</i> EOs obtained from leaves and flowers (fresh and dried plant material) were evaluated using hydrodistillation (HD), steam distillation (SD), simultaneous distillation–extraction (SDE), and solid-phase microextraction (SPME) techniques. Antimicrobial activity (minimum inhibitory concentration, MIC) and antioxidant capacity (half-maximal inhibitory concentration, IC<sub>50</sub>) were determined. The identification and quantification of the compounds present in the EOs were conducted by gas chromatography coupled to mass spectrometry (GC-MS). The main secondary metabolites identified in most samples obtained by different extraction techniques included phenol (~18%), 1S-α-pinene (~15%), β-phellandrene (~13%), β-pinene (~12%), 4-terpineol (~10%), γ-terpinene (~10%), trans-nerolidol (~8%), limonene (~8%), and β-thujene (~6%). EOs obtained by HD, SD, and SDE exhibited antioxidant activity, with IC<sub>50</sub> values between 621.7 and 696.6 µg/mL. Additionally, the EOs demonstrated bactericidal activity against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, with MIC values of 5.0 and 45 µg/mL, respectively. <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> did not show antimicrobial susceptibility to EOs. This study constitutes the first evaluation of <i>Pentacalia vaccinioides</i> EOs, demonstrating their bioactive potential and the relevance of the extraction method. The findings highlight this species as a promising source of natural compounds for therapeutic and preservative applications, depending on the type of plant material and extraction technique used. Future research should investigate how microclimatic conditions and plant development affect the chemical composition and elucidate the molecular mechanisms behind the observed bioactivities to better understand their cellular actions. Furthermore, the evaluation of the applications of EOs and hydrolates in the pharmaceutical and food industries, along with the exploration of the bioactive potential of extraction-derived hydrolates, offers a promising avenue to maximize plant utility.https://www.mdpi.com/2297-8739/12/1/9<i>Pentacalia vaccinioides</i>antioxidantantimicrobialessential oilvolatile compoundshydrodistillation |
| spellingShingle | Luis G. Sequeda-Castañeda María A. Castellanos-Gómez Carlos L. A. Céspedes-Acuña Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities Separations <i>Pentacalia vaccinioides</i> antioxidant antimicrobial essential oil volatile compounds hydrodistillation |
| title | Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities |
| title_full | Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities |
| title_fullStr | Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities |
| title_full_unstemmed | Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities |
| title_short | Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities |
| title_sort | bioactive properties of i pentacalia vaccinioides i kunth cuatrec asteraceae essential oils evaluation of antimicrobial and antioxidant activities |
| topic | <i>Pentacalia vaccinioides</i> antioxidant antimicrobial essential oil volatile compounds hydrodistillation |
| url | https://www.mdpi.com/2297-8739/12/1/9 |
| work_keys_str_mv | AT luisgsequedacastaneda bioactivepropertiesofipentacaliavaccinioidesikunthcuatrecasteraceaeessentialoilsevaluationofantimicrobialandantioxidantactivities AT mariaacastellanosgomez bioactivepropertiesofipentacaliavaccinioidesikunthcuatrecasteraceaeessentialoilsevaluationofantimicrobialandantioxidantactivities AT carloslacespedesacuna bioactivepropertiesofipentacaliavaccinioidesikunthcuatrecasteraceaeessentialoilsevaluationofantimicrobialandantioxidantactivities |