Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model
Purpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was per...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/4046597 |
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| _version_ | 1832564953531285504 |
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| author | Tilda Barliya Ron Ofri Shai Sandalon Dov Weinberger Tami Livnat |
| author_facet | Tilda Barliya Ron Ofri Shai Sandalon Dov Weinberger Tami Livnat |
| author_sort | Tilda Barliya |
| collection | DOAJ |
| description | Purpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was performed 0, 3, 7, 14, and 21 days postoperatively. ERGs were recorded pre- and 7 and 21 days postoperatively. Eyes were harvested on day 21 and evaluated immunohistochemically (IHC) for remodeling of second- and third-order neurons. Results. Retinal reattachment occurred within two weeks following surgery. No attenuation was observed in the photopic or scotopic a- and b-waves. A secondary wavefront on the descending slope of the scotopic b-wave was the only ERG result that was attenuated in detached retinas. IHC demonstrated anatomical changes in both ON and OFF bipolar cells. Bassoon staining was observed in the remodeled dendrites. Amacrine and horizontal cells did not alter, but Muller cells were clearly reactive with marked extension. Conclusion. Retinal detachment and reattachment were associated with functional and anatomical changes. Exploring the significance of the secondary scotopic wavefront and its association with the remodeling of 2nd- and 3rd-order neurons will shade more light on functional changes and recovery of the retina. |
| format | Article |
| id | doaj-art-5138ee00180b4db0a0891b6c3ac33d6a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-5138ee00180b4db0a0891b6c3ac33d6a2025-02-03T01:09:45ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/40465974046597Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit ModelTilda Barliya0Ron Ofri1Shai Sandalon2Dov Weinberger3Tami Livnat4Division of Ophthalmology, Rabin Medical Center, Beilinson Campus, Petah Tikva, IsraelKoret School of Veterinary Medicine, The R. H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelKoret School of Veterinary Medicine, The R. H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDivision of Ophthalmology, Rabin Medical Center, Beilinson Campus, Petah Tikva, IsraelLaboratory of Eye Research, Felsenstein Medical Research Center (FMRC), Rabin Medical Center, Petah Tikva, IsraelPurpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was performed 0, 3, 7, 14, and 21 days postoperatively. ERGs were recorded pre- and 7 and 21 days postoperatively. Eyes were harvested on day 21 and evaluated immunohistochemically (IHC) for remodeling of second- and third-order neurons. Results. Retinal reattachment occurred within two weeks following surgery. No attenuation was observed in the photopic or scotopic a- and b-waves. A secondary wavefront on the descending slope of the scotopic b-wave was the only ERG result that was attenuated in detached retinas. IHC demonstrated anatomical changes in both ON and OFF bipolar cells. Bassoon staining was observed in the remodeled dendrites. Amacrine and horizontal cells did not alter, but Muller cells were clearly reactive with marked extension. Conclusion. Retinal detachment and reattachment were associated with functional and anatomical changes. Exploring the significance of the secondary scotopic wavefront and its association with the remodeling of 2nd- and 3rd-order neurons will shade more light on functional changes and recovery of the retina.http://dx.doi.org/10.1155/2017/4046597 |
| spellingShingle | Tilda Barliya Ron Ofri Shai Sandalon Dov Weinberger Tami Livnat Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model Journal of Ophthalmology |
| title | Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model |
| title_full | Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model |
| title_fullStr | Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model |
| title_full_unstemmed | Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model |
| title_short | Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model |
| title_sort | changes in retinal function and cellular remodeling following experimental retinal detachment in a rabbit model |
| url | http://dx.doi.org/10.1155/2017/4046597 |
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