Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning

Osteosarcoma is the most common primary bone tumor with high malignancy. It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis. Accordingly, the multimodal microscopic imaging diagnosis system constructed by bright field, spontaneo...

Full description

Saved in:
Bibliographic Details
Main Authors: Zihan Wang, Jinjin Wu, Chenbei Li, Bing Wang, Qingxia Wu, Lan Li, Huijie Wang, Chao Tu, Jianhua Yin
Format: Article
Language:English
Published: World Scientific Publishing 2025-03-01
Series:Journal of Innovative Optical Health Sciences
Subjects:
Online Access:https://www.worldscientific.com/doi/10.1142/S1793545823430010
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850046668806815744
author Zihan Wang
Jinjin Wu
Chenbei Li
Bing Wang
Qingxia Wu
Lan Li
Huijie Wang
Chao Tu
Jianhua Yin
author_facet Zihan Wang
Jinjin Wu
Chenbei Li
Bing Wang
Qingxia Wu
Lan Li
Huijie Wang
Chao Tu
Jianhua Yin
author_sort Zihan Wang
collection DOAJ
description Osteosarcoma is the most common primary bone tumor with high malignancy. It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis. Accordingly, the multimodal microscopic imaging diagnosis system constructed by bright field, spontaneous fluorescence and polarized light microscopic imaging was used to study the pathological mechanism of osteosarcoma from the tissue microenvironment level and achieve rapid and accurate diagnosis. First, the multimodal microscopic images of normal and osteosarcoma tissue slices were collected to characterize the overall morphology of the tissue microenvironment of the samples, the arrangement structure of collagen fibers and the content and distribution of endogenous fluorescent substances. Second, based on the correlation and complementarity of the feature information contained in the three single-mode images, combined with convolutional neural network (CNN) and image fusion methods, a multimodal intelligent diagnosis model was constructed to effectively improve the information utilization and diagnosis accuracy. The accuracy and true positivity of the multimodal diagnostic model were significantly improved to 0.8495 and 0.9412, respectively, compared to those of the single-modal models. Besides, the difference of tissue microenvironments before and after cancerization can be used as a basis for cancer diagnosis, and the information extraction and intelligent diagnosis of osteosarcoma tissue can be achieved by using multimodal microscopic imaging technology combined with deep learning, which significantly promoted the application of tissue microenvironment in pathological examination. This diagnostic system relies on its advantages of simple operation, high efficiency and accuracy and high cost-effectiveness, and has enormous clinical application potential and research significance.
format Article
id doaj-art-51181a10a3d94e7bb0a88b74cc63ff0b
institution DOAJ
issn 1793-5458
1793-7205
language English
publishDate 2025-03-01
publisher World Scientific Publishing
record_format Article
series Journal of Innovative Optical Health Sciences
spelling doaj-art-51181a10a3d94e7bb0a88b74cc63ff0b2025-08-20T02:54:23ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052025-03-01180210.1142/S1793545823430010Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learningZihan Wang0Jinjin Wu1Chenbei Li2Bing Wang3Qingxia Wu4Lan Li5Huijie Wang6Chao Tu7Jianhua Yin8Department of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaDepartment of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaDepartment of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P. R. ChinaDepartment of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaDepartment of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaDepartment of Pathology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P. R. ChinaDepartment of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaDepartment of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P. R. ChinaDepartment of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, P. R. ChinaOsteosarcoma is the most common primary bone tumor with high malignancy. It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis. Accordingly, the multimodal microscopic imaging diagnosis system constructed by bright field, spontaneous fluorescence and polarized light microscopic imaging was used to study the pathological mechanism of osteosarcoma from the tissue microenvironment level and achieve rapid and accurate diagnosis. First, the multimodal microscopic images of normal and osteosarcoma tissue slices were collected to characterize the overall morphology of the tissue microenvironment of the samples, the arrangement structure of collagen fibers and the content and distribution of endogenous fluorescent substances. Second, based on the correlation and complementarity of the feature information contained in the three single-mode images, combined with convolutional neural network (CNN) and image fusion methods, a multimodal intelligent diagnosis model was constructed to effectively improve the information utilization and diagnosis accuracy. The accuracy and true positivity of the multimodal diagnostic model were significantly improved to 0.8495 and 0.9412, respectively, compared to those of the single-modal models. Besides, the difference of tissue microenvironments before and after cancerization can be used as a basis for cancer diagnosis, and the information extraction and intelligent diagnosis of osteosarcoma tissue can be achieved by using multimodal microscopic imaging technology combined with deep learning, which significantly promoted the application of tissue microenvironment in pathological examination. This diagnostic system relies on its advantages of simple operation, high efficiency and accuracy and high cost-effectiveness, and has enormous clinical application potential and research significance.https://www.worldscientific.com/doi/10.1142/S1793545823430010Multimodal imagingimage fusiondeep learningosteosarcomaintelligent diagnosis
spellingShingle Zihan Wang
Jinjin Wu
Chenbei Li
Bing Wang
Qingxia Wu
Lan Li
Huijie Wang
Chao Tu
Jianhua Yin
Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
Journal of Innovative Optical Health Sciences
Multimodal imaging
image fusion
deep learning
osteosarcoma
intelligent diagnosis
title Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
title_full Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
title_fullStr Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
title_full_unstemmed Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
title_short Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
title_sort diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning
topic Multimodal imaging
image fusion
deep learning
osteosarcoma
intelligent diagnosis
url https://www.worldscientific.com/doi/10.1142/S1793545823430010
work_keys_str_mv AT zihanwang diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT jinjinwu diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT chenbeili diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT bingwang diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT qingxiawu diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT lanli diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT huijiewang diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT chaotu diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning
AT jianhuayin diagnosisofosteosarcomabasedonmultimodalmicroscopicimaginganddeeplearning