Murine model for chronic rhinosinusitis: an interventional study
Abstract Background Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the sinonasal tract. To understand this disease entity and develop targeted treatments, a reproducible animal model is paramount. Aims/objectives To optimize a murine model of eosinophilic CRS by establishing bench...
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SAGE Publishing
2023-04-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
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| Online Access: | https://doi.org/10.1186/s40463-023-00637-6 |
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| author | Amr F. Hamour John JW. Lee Ewa Wasilewski Eric Monteiro John M. Lee Allan Vescan Lakshmi P. Kotra |
| author_facet | Amr F. Hamour John JW. Lee Ewa Wasilewski Eric Monteiro John M. Lee Allan Vescan Lakshmi P. Kotra |
| author_sort | Amr F. Hamour |
| collection | DOAJ |
| description | Abstract Background Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the sinonasal tract. To understand this disease entity and develop targeted treatments, a reproducible animal model is paramount. Aims/objectives To optimize a murine model of eosinophilic CRS by establishing benchmark histological markers and validate its fidelity in evaluating intranasal treatments. Material and methods Forty-five Balb/c mice were included in the 7-week protocol. Experimental animals (n = 20) were induced a CRS disease state upon receiving intraperitoneal sensitization with ovalbumin (OVA), followed by intranasal OVA with Aspergillus oryzae protease. Analysis of complete blood count with differential, peripheral blood smear, and histological markers from the nasal cavity mucosa were performed. CRS mice were additionally treated with intranasal saline (n = 5) or mometasone (n = 10) and compared with control groups of untreated CRS (n = 5) and healthy (n = 5) mice after week 7. Results Histological analysis of experimental animal nasal mucosa revealed significantly higher levels of eosinophilic tissue infiltration/degranulation, hyaline droplets, Charcot–Leyden crystals, and respiratory epithelial thickness compared to healthy controls. Treatment with mometasone significantly reversed the histopathological changes observed in CRS mice. Conclusion and significance This murine model induced substantial local eosinophilic inflammation within sinonasal mucosa, that was reversible with mometasone. This model may be used to evaluate the efficacy of therapeutics designed to target CRS. |
| format | Article |
| id | doaj-art-50b8b4b6903746f49dc689ef3991f683 |
| institution | Kabale University |
| issn | 1916-0216 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj-art-50b8b4b6903746f49dc689ef3991f6832025-02-03T01:08:31ZengSAGE PublishingJournal of Otolaryngology - Head and Neck Surgery1916-02162023-04-015211810.1186/s40463-023-00637-6Murine model for chronic rhinosinusitis: an interventional studyAmr F. Hamour0John JW. Lee1Ewa Wasilewski2Eric Monteiro3John M. Lee4Allan Vescan5Lakshmi P. Kotra6Department of Otolaryngology – Head and Neck Surgery, Temerty Faculty of Medicine, University of TorontoDepartment of Otolaryngology – Head and Neck Surgery, Temerty Faculty of Medicine, University of TorontoCentre for Cannabinoid Therapeutics and Centre for Molecular Design and Preformulations, Krembil Research Institute, University Health NetworkDepartment of Otolaryngology – Head and Neck Surgery, Temerty Faculty of Medicine, University of TorontoDepartment of Otolaryngology – Head and Neck Surgery, Temerty Faculty of Medicine, University of TorontoDepartment of Otolaryngology – Head and Neck Surgery, Temerty Faculty of Medicine, University of TorontoCentre for Cannabinoid Therapeutics and Centre for Molecular Design and Preformulations, Krembil Research Institute, University Health NetworkAbstract Background Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the sinonasal tract. To understand this disease entity and develop targeted treatments, a reproducible animal model is paramount. Aims/objectives To optimize a murine model of eosinophilic CRS by establishing benchmark histological markers and validate its fidelity in evaluating intranasal treatments. Material and methods Forty-five Balb/c mice were included in the 7-week protocol. Experimental animals (n = 20) were induced a CRS disease state upon receiving intraperitoneal sensitization with ovalbumin (OVA), followed by intranasal OVA with Aspergillus oryzae protease. Analysis of complete blood count with differential, peripheral blood smear, and histological markers from the nasal cavity mucosa were performed. CRS mice were additionally treated with intranasal saline (n = 5) or mometasone (n = 10) and compared with control groups of untreated CRS (n = 5) and healthy (n = 5) mice after week 7. Results Histological analysis of experimental animal nasal mucosa revealed significantly higher levels of eosinophilic tissue infiltration/degranulation, hyaline droplets, Charcot–Leyden crystals, and respiratory epithelial thickness compared to healthy controls. Treatment with mometasone significantly reversed the histopathological changes observed in CRS mice. Conclusion and significance This murine model induced substantial local eosinophilic inflammation within sinonasal mucosa, that was reversible with mometasone. This model may be used to evaluate the efficacy of therapeutics designed to target CRS.https://doi.org/10.1186/s40463-023-00637-6Animal modelChronic rhinosinusitisEosinophilic chronic rhinosinusitisHistological markersInflammation |
| spellingShingle | Amr F. Hamour John JW. Lee Ewa Wasilewski Eric Monteiro John M. Lee Allan Vescan Lakshmi P. Kotra Murine model for chronic rhinosinusitis: an interventional study Journal of Otolaryngology - Head and Neck Surgery Animal model Chronic rhinosinusitis Eosinophilic chronic rhinosinusitis Histological markers Inflammation |
| title | Murine model for chronic rhinosinusitis: an interventional study |
| title_full | Murine model for chronic rhinosinusitis: an interventional study |
| title_fullStr | Murine model for chronic rhinosinusitis: an interventional study |
| title_full_unstemmed | Murine model for chronic rhinosinusitis: an interventional study |
| title_short | Murine model for chronic rhinosinusitis: an interventional study |
| title_sort | murine model for chronic rhinosinusitis an interventional study |
| topic | Animal model Chronic rhinosinusitis Eosinophilic chronic rhinosinusitis Histological markers Inflammation |
| url | https://doi.org/10.1186/s40463-023-00637-6 |
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