Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon
Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infe...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3074803 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556464867115008 |
|---|---|
| author | William Yavo Abibatou Konaté Denise Patricia Mawili-Mboumba Fulgence Kondo Kassi Marie L. Tshibola Mbuyi Etienne Kpongbo Angora Eby I. Hervé Menan Marielle K. Bouyou-Akotet |
| author_facet | William Yavo Abibatou Konaté Denise Patricia Mawili-Mboumba Fulgence Kondo Kassi Marie L. Tshibola Mbuyi Etienne Kpongbo Angora Eby I. Hervé Menan Marielle K. Bouyou-Akotet |
| author_sort | William Yavo |
| collection | DOAJ |
| description | Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d’Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d’Ivoire (64.6%) and in Gabon (56.6%). For msp2, the 3D7 alleles were more prevalent (>70% in both countries) compared to FC27 alleles. In Côte d’Ivoire, the frequencies of multiple infections with msp1 (45.1%) and msp2 (40.3%) were higher than those found for isolates from Gabon, that is, 30.2% with msp1 and 31.4% with msp2. The overall complexity of infection was 1.66 (SD = 0.79) in Côte d’Ivoire and 1.58 (SD = 0.83) in Gabon. It decreased with age in Côte d’Ivoire in contrast to Gabon. Conclusion. Differences observed in some allelic families and in complexity profile may suggest an impact of epidemiological facies as well as immunological response on genetic variability of P. falciparum. |
| format | Article |
| id | doaj-art-50a76a709b384ed7aab2737c61caca6b |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-50a76a709b384ed7aab2737c61caca6b2025-02-03T05:45:23ZengWileyJournal of Parasitology Research2090-00232090-00312016-01-01201610.1155/2016/30748033074803Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus GabonWilliam Yavo0Abibatou Konaté1Denise Patricia Mawili-Mboumba2Fulgence Kondo Kassi3Marie L. Tshibola Mbuyi4Etienne Kpongbo Angora5Eby I. Hervé Menan6Marielle K. Bouyou-Akotet7Malaria Research and Control Centre, National Institute of Public Health, BPV 47, Abidjan, Côte d’IvoireMalaria Research and Control Centre, National Institute of Public Health, BPV 47, Abidjan, Côte d’IvoireFaculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, GabonFaculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’IvoireFaculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, GabonFaculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’IvoireFaculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’IvoireFaculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, GabonIntroduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d’Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d’Ivoire (64.6%) and in Gabon (56.6%). For msp2, the 3D7 alleles were more prevalent (>70% in both countries) compared to FC27 alleles. In Côte d’Ivoire, the frequencies of multiple infections with msp1 (45.1%) and msp2 (40.3%) were higher than those found for isolates from Gabon, that is, 30.2% with msp1 and 31.4% with msp2. The overall complexity of infection was 1.66 (SD = 0.79) in Côte d’Ivoire and 1.58 (SD = 0.83) in Gabon. It decreased with age in Côte d’Ivoire in contrast to Gabon. Conclusion. Differences observed in some allelic families and in complexity profile may suggest an impact of epidemiological facies as well as immunological response on genetic variability of P. falciparum.http://dx.doi.org/10.1155/2016/3074803 |
| spellingShingle | William Yavo Abibatou Konaté Denise Patricia Mawili-Mboumba Fulgence Kondo Kassi Marie L. Tshibola Mbuyi Etienne Kpongbo Angora Eby I. Hervé Menan Marielle K. Bouyou-Akotet Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon Journal of Parasitology Research |
| title | Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon |
| title_full | Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon |
| title_fullStr | Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon |
| title_full_unstemmed | Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon |
| title_short | Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon |
| title_sort | genetic polymorphism of msp1 and msp2 in plasmodium falciparum isolates from cote d ivoire versus gabon |
| url | http://dx.doi.org/10.1155/2016/3074803 |
| work_keys_str_mv | AT williamyavo geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT abibatoukonate geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT denisepatriciamawilimboumba geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT fulgencekondokassi geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT marieltshibolambuyi geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT etiennekpongboangora geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT ebyihervemenan geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon AT mariellekbouyouakotet geneticpolymorphismofmsp1andmsp2inplasmodiumfalciparumisolatesfromcotedivoireversusgabon |