Microarray Profiling of Lymphocytes in Internal Diseases With an Altered Immune Response: Potential and Methodology
Recently it has become possible to investigate expression of all human genes with microarray technique. The authors provide arguments to consider peripheral white blood cells and in particular lymphocytes as a model for the investigation of pathophysiology of asthma, RA, and SLE diseases in which in...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI.2005.317 |
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| _version_ | 1832561083652505600 |
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| author | Anatoliy Gladkevich S. Adriaan Nelemans Henk F. Kauffman Jakob Korf |
| author_facet | Anatoliy Gladkevich S. Adriaan Nelemans Henk F. Kauffman Jakob Korf |
| author_sort | Anatoliy Gladkevich |
| collection | DOAJ |
| description | Recently it has become possible to investigate
expression of all human genes with microarray technique. The
authors provide arguments to consider peripheral white blood cells
and in particular lymphocytes as a model for the investigation of
pathophysiology of asthma, RA, and SLE diseases in which
inflammation is a major component. Lymphocytes are an alternative
to tissue biopsies that are most often difficult to collect
systematically. Lymphocytes express more than 75% of the human
genome, and, being an important part of the immune system, they
play a central role in the pathogenesis of asthma, RA, and SLE.
Here we review alterations of gene expression in lymphocytes and
methodological aspects of the microarray technique in these
diseases. Lymphocytic genes may become activated because of a
general nonspecific versus disease-specific mechanism.
The authors suppose that in these diseases microarray profiles of
gene expression in lymphocytes can be disease specific, rather
than inflammation specific. Some potentials and pitfalls of the
array technologies are discussed. Optimal clinical designs aimed
to identify disease-specific genes are proposed. Lymphocytes can
be explored for research, diagnostic, and possible treatment
purposes in these diseases, but their precise
value should be clarified in future investigation. |
| format | Article |
| id | doaj-art-50a63eb95e70402e95451741ea9982d7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-50a63eb95e70402e95451741ea9982d72025-02-03T01:26:01ZengWileyMediators of Inflammation0962-93511466-18612005-01-012005631733010.1155/MI.2005.317Microarray Profiling of Lymphocytes in Internal Diseases With an Altered Immune Response: Potential and MethodologyAnatoliy Gladkevich0S. Adriaan Nelemans1Henk F. Kauffman2Jakob Korf3Departments of Psychiatry, Molecular Pharmacology, and Allergology, University Medical Center of Groningen, Hanzeplein 1, PO Box 30001, Groningen 9700 RB, The NetherlandsDepartments of Psychiatry, Molecular Pharmacology, and Allergology, University Medical Center of Groningen, Hanzeplein 1, PO Box 30001, Groningen 9700 RB, The NetherlandsDepartments of Psychiatry, Molecular Pharmacology, and Allergology, University Medical Center of Groningen, Hanzeplein 1, PO Box 30001, Groningen 9700 RB, The NetherlandsDepartments of Psychiatry, Molecular Pharmacology, and Allergology, University Medical Center of Groningen, Hanzeplein 1, PO Box 30001, Groningen 9700 RB, The NetherlandsRecently it has become possible to investigate expression of all human genes with microarray technique. The authors provide arguments to consider peripheral white blood cells and in particular lymphocytes as a model for the investigation of pathophysiology of asthma, RA, and SLE diseases in which inflammation is a major component. Lymphocytes are an alternative to tissue biopsies that are most often difficult to collect systematically. Lymphocytes express more than 75% of the human genome, and, being an important part of the immune system, they play a central role in the pathogenesis of asthma, RA, and SLE. Here we review alterations of gene expression in lymphocytes and methodological aspects of the microarray technique in these diseases. Lymphocytic genes may become activated because of a general nonspecific versus disease-specific mechanism. The authors suppose that in these diseases microarray profiles of gene expression in lymphocytes can be disease specific, rather than inflammation specific. Some potentials and pitfalls of the array technologies are discussed. Optimal clinical designs aimed to identify disease-specific genes are proposed. Lymphocytes can be explored for research, diagnostic, and possible treatment purposes in these diseases, but their precise value should be clarified in future investigation.http://dx.doi.org/10.1155/MI.2005.317 |
| spellingShingle | Anatoliy Gladkevich S. Adriaan Nelemans Henk F. Kauffman Jakob Korf Microarray Profiling of Lymphocytes in Internal Diseases With an Altered Immune Response: Potential and Methodology Mediators of Inflammation |
| title | Microarray Profiling of Lymphocytes in Internal Diseases
With an Altered Immune Response: Potential and
Methodology |
| title_full | Microarray Profiling of Lymphocytes in Internal Diseases
With an Altered Immune Response: Potential and
Methodology |
| title_fullStr | Microarray Profiling of Lymphocytes in Internal Diseases
With an Altered Immune Response: Potential and
Methodology |
| title_full_unstemmed | Microarray Profiling of Lymphocytes in Internal Diseases
With an Altered Immune Response: Potential and
Methodology |
| title_short | Microarray Profiling of Lymphocytes in Internal Diseases
With an Altered Immune Response: Potential and
Methodology |
| title_sort | microarray profiling of lymphocytes in internal diseases with an altered immune response potential and methodology |
| url | http://dx.doi.org/10.1155/MI.2005.317 |
| work_keys_str_mv | AT anatoliygladkevich microarrayprofilingoflymphocytesininternaldiseaseswithanalteredimmuneresponsepotentialandmethodology AT sadriaannelemans microarrayprofilingoflymphocytesininternaldiseaseswithanalteredimmuneresponsepotentialandmethodology AT henkfkauffman microarrayprofilingoflymphocytesininternaldiseaseswithanalteredimmuneresponsepotentialandmethodology AT jakobkorf microarrayprofilingoflymphocytesininternaldiseaseswithanalteredimmuneresponsepotentialandmethodology |