Physiological Response of Olive Trees Under <i>Xylella fastidiosa</i> Infection and Thymol Therapy Monitored Through Advanced IoT Sensors

Since its first detection in 2013, <i>Xylella fastidiosa</i> subsp. <i>pauca</i> (<i>Xfp</i>) has caused a devastating Olive Quick Decline Syndrome (OQDS) outbreak in Southern Italy. Effective disease surveillance and treatment strategies are urgently needed to mi...

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Main Authors: Claudia Cagnarini, Paolo De Angelis, Dario Liberati, Riccardo Valentini, Valentina Falanga, Franco Valentini, Crescenza Dongiovanni, Mauro Carrieri, Maria Vincenza Chiriacò
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/9/1380
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Summary:Since its first detection in 2013, <i>Xylella fastidiosa</i> subsp. <i>pauca</i> (<i>Xfp</i>) has caused a devastating Olive Quick Decline Syndrome (OQDS) outbreak in Southern Italy. Effective disease surveillance and treatment strategies are urgently needed to mitigate its impact. This study investigates the short-term (1.5 years) effects of thymol-based treatments on infected olive trees of the susceptible cultivar Cellina di Nardò in two orchards in Salento, Apulia region. Twenty trees per trial received a 3% thymol solution either alone or encapsulated in a cellulose nanoparticle carrier. Over two years, sap flux density and canopy-transmitted solar radiation were monitored using TreeTalker sensors, and spectral greenness indices were calculated. <i>Xfp</i> cell concentrations in plant tissues were quantified via qPCR. Neither thymol treatment halted disease progression nor significantly reduced bacterial load, though the <i>Xfp</i> cell concentration reduction increased over time in the preventive trial. Symptomatic trees exhibited increased sap flux density, though the treatment mitigated this effect in the curative trial. Greenness indices remained lower in infected trees, but the response to symptom severity was delayed. These findings underscore the need for longer-term studies, investigation of synergistic effects with other phytocompounds, and integration of real-time sensor data into adaptive disease management protocols.
ISSN:2223-7747