Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis
Aim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), ob...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2013/836121 |
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| author | Dogan Atilgan Bekir S. Parlaktas Nihat Uluocak Fikret Erdemir Sahin Kilic Unal Erkorkmaz Huseyin Ozyurt Fatma Markoc |
| author_facet | Dogan Atilgan Bekir S. Parlaktas Nihat Uluocak Fikret Erdemir Sahin Kilic Unal Erkorkmaz Huseyin Ozyurt Fatma Markoc |
| author_sort | Dogan Atilgan |
| collection | DOAJ |
| description | Aim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), obesity + MLT group (Group 3), and weight loss group (Group 4). Rats were weighed at the beginning and at the end of the study. Bilateral orchiectomy was performed and 5 cc blood samples were obtained from all of the rats. Superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were analysed in the testicular tissues and serum. Spermatogenesis was evaluated with the Johnsen scoring system. Results. The testicular tissue and serum levels of MDA, PC, and SOD activity were increased in the obesity group in comparison to the sham operated group (P<0.05). Weight loss and melatonin treatment ameliorated MDA, PC, and SOD levels in testicular tissue and serum significantly (P<0.05). There was no significant difference between groups in terms of mean Johnsen score (P=0.727). Conclusion. Experimentally created obesity caused oxidative stress and both melatonin and weight loss reduced oxidative stress parameters in rat testes. |
| format | Article |
| id | doaj-art-506f9049564c4c5a97ba1185534a6b0a |
| institution | Kabale University |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-506f9049564c4c5a97ba1185534a6b0a2025-02-03T01:32:03ZengWileyAdvances in Urology1687-63691687-63772013-01-01201310.1155/2013/836121836121Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat TestisDogan Atilgan0Bekir S. Parlaktas1Nihat Uluocak2Fikret Erdemir3Sahin Kilic4Unal Erkorkmaz5Huseyin Ozyurt6Fatma Markoc7Gaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, TurkeySakarya University, Faculty of Medicine, Department of Biostatistics and Medical informatics, 54100 Sakarya, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Biochemistry, 60100 Tokat, TurkeyGaziosmanpasa University, Faculty of Medicine, Department of Pathology, 60100 Tokat, TurkeyAim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), obesity + MLT group (Group 3), and weight loss group (Group 4). Rats were weighed at the beginning and at the end of the study. Bilateral orchiectomy was performed and 5 cc blood samples were obtained from all of the rats. Superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were analysed in the testicular tissues and serum. Spermatogenesis was evaluated with the Johnsen scoring system. Results. The testicular tissue and serum levels of MDA, PC, and SOD activity were increased in the obesity group in comparison to the sham operated group (P<0.05). Weight loss and melatonin treatment ameliorated MDA, PC, and SOD levels in testicular tissue and serum significantly (P<0.05). There was no significant difference between groups in terms of mean Johnsen score (P=0.727). Conclusion. Experimentally created obesity caused oxidative stress and both melatonin and weight loss reduced oxidative stress parameters in rat testes.http://dx.doi.org/10.1155/2013/836121 |
| spellingShingle | Dogan Atilgan Bekir S. Parlaktas Nihat Uluocak Fikret Erdemir Sahin Kilic Unal Erkorkmaz Huseyin Ozyurt Fatma Markoc Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis Advances in Urology |
| title | Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis |
| title_full | Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis |
| title_fullStr | Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis |
| title_full_unstemmed | Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis |
| title_short | Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis |
| title_sort | weight loss and melatonin reduce obesity induced oxidative damage in rat testis |
| url | http://dx.doi.org/10.1155/2013/836121 |
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