Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant

Catechins, renowned for their health benefits, have unexamined environmental impacts. This study assessed the toxicity of crude catechin and catechin hydrate on invertebrate larvae, plant, and microalgae. The survival rates of <i>Daphnia magna</i> Straus and <i>Artemia salina</i...

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Main Authors: Khawaja Muhammad Imran Bashir, Hye-Ryeon An, Bertoka Fajar Surya Perwira Negara, Gabriel Tirtawijaya, Maria Dyah Nur Meinita, Jae-Hak Sohn, Dicky Harwanto, Jae-Suk Choi
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Toxins
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Online Access:https://www.mdpi.com/2072-6651/17/5/244
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author Khawaja Muhammad Imran Bashir
Hye-Ryeon An
Bertoka Fajar Surya Perwira Negara
Gabriel Tirtawijaya
Maria Dyah Nur Meinita
Jae-Hak Sohn
Dicky Harwanto
Jae-Suk Choi
author_facet Khawaja Muhammad Imran Bashir
Hye-Ryeon An
Bertoka Fajar Surya Perwira Negara
Gabriel Tirtawijaya
Maria Dyah Nur Meinita
Jae-Hak Sohn
Dicky Harwanto
Jae-Suk Choi
author_sort Khawaja Muhammad Imran Bashir
collection DOAJ
description Catechins, renowned for their health benefits, have unexamined environmental impacts. This study assessed the toxicity of crude catechin and catechin hydrate on invertebrate larvae, plant, and microalgae. The survival rates of <i>Daphnia magna</i> Straus and <i>Artemia salina</i> L. were monitored every 24 h over a three-day period. The germination rate and radicle length of <i>Lactuca sativa</i> L. was measured every 24 h for four days. Inhibitory effects were evaluated in both freshwater and seawater cultures of <i>Chlorella vulgaris</i> Beijerinck, with cell density recorded every 24 h and yield inhibition calculated after 96 h. Results indicated that increasing catechin concentration and exposure duration decreased the survival rate of <i>D. magna</i> and <i>A. salina</i>. <i>Daphnia magna</i> was more sensitive to catechins than <i>A. salina</i>, with 24 h lethal concentration 50 (LC-50) values of 1174 µg/mL compared to 1895 µg/mL for crude catechin, and 54 µg/mL compared to 153 µg/mL for catechin hydrate. The germination rate and radicle length of <i>L. sativa</i>, along with the cell density of <i>C. vulgaris</i>, decreased with increasing catechin concentration, but remained higher even after prolonged exposure. At low catechin concentrations, <i>C. vulgaris</i> cell density exceeded control levels. This study demonstrates that catechins in aquatic environments can significantly impact ecosystems. At certain concentrations, catechins are toxic and potentially lethal to aquatic organisms. Conversely, at lower concentrations, catechins may promote microalgal growth, suggesting a fertilizing effect. Understanding these dynamics is crucial for maintaining the stability of aquatic ecosystems.
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publisher MDPI AG
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series Toxins
spelling doaj-art-504890fefb514b16be9ebced3d642eba2025-08-20T03:47:58ZengMDPI AGToxins2072-66512025-05-0117524410.3390/toxins17050244Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial PlantKhawaja Muhammad Imran Bashir0Hye-Ryeon An1Bertoka Fajar Surya Perwira Negara2Gabriel Tirtawijaya3Maria Dyah Nur Meinita4Jae-Hak Sohn5Dicky Harwanto6Jae-Suk Choi7Department of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaDepartment of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaDepartment of Marine Science, University of Bengkulu, Jl. W.R Soepratman, Bengkulu 38371, IndonesiaFaculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut Surabaya, Surabaya 60292, IndonesiaFaculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53123, IndonesiaDepartment of Food Science and Culinary Arts, College of Health and Welfare, Silla University, Busan 46958, Republic of KoreaFaculty of Fisheries and Marine Science, Diponegoro University, Semarang 50275, IndonesiaDepartment of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaCatechins, renowned for their health benefits, have unexamined environmental impacts. This study assessed the toxicity of crude catechin and catechin hydrate on invertebrate larvae, plant, and microalgae. The survival rates of <i>Daphnia magna</i> Straus and <i>Artemia salina</i> L. were monitored every 24 h over a three-day period. The germination rate and radicle length of <i>Lactuca sativa</i> L. was measured every 24 h for four days. Inhibitory effects were evaluated in both freshwater and seawater cultures of <i>Chlorella vulgaris</i> Beijerinck, with cell density recorded every 24 h and yield inhibition calculated after 96 h. Results indicated that increasing catechin concentration and exposure duration decreased the survival rate of <i>D. magna</i> and <i>A. salina</i>. <i>Daphnia magna</i> was more sensitive to catechins than <i>A. salina</i>, with 24 h lethal concentration 50 (LC-50) values of 1174 µg/mL compared to 1895 µg/mL for crude catechin, and 54 µg/mL compared to 153 µg/mL for catechin hydrate. The germination rate and radicle length of <i>L. sativa</i>, along with the cell density of <i>C. vulgaris</i>, decreased with increasing catechin concentration, but remained higher even after prolonged exposure. At low catechin concentrations, <i>C. vulgaris</i> cell density exceeded control levels. This study demonstrates that catechins in aquatic environments can significantly impact ecosystems. At certain concentrations, catechins are toxic and potentially lethal to aquatic organisms. Conversely, at lower concentrations, catechins may promote microalgal growth, suggesting a fertilizing effect. Understanding these dynamics is crucial for maintaining the stability of aquatic ecosystems.https://www.mdpi.com/2072-6651/17/5/244algaecell densityeco-friendlygerminationplanktontoxicity
spellingShingle Khawaja Muhammad Imran Bashir
Hye-Ryeon An
Bertoka Fajar Surya Perwira Negara
Gabriel Tirtawijaya
Maria Dyah Nur Meinita
Jae-Hak Sohn
Dicky Harwanto
Jae-Suk Choi
Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
Toxins
algae
cell density
eco-friendly
germination
plankton
toxicity
title Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
title_full Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
title_fullStr Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
title_full_unstemmed Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
title_short Toxicity Assessment of Catechins on Representative Aquatic Organisms and Terrestrial Plant
title_sort toxicity assessment of catechins on representative aquatic organisms and terrestrial plant
topic algae
cell density
eco-friendly
germination
plankton
toxicity
url https://www.mdpi.com/2072-6651/17/5/244
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