The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the eff...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/380948 |
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| author | Barbora Safrankova Silvie Gajdova Lukas Kubala |
| author_facet | Barbora Safrankova Silvie Gajdova Lukas Kubala |
| author_sort | Barbora Safrankova |
| collection | DOAJ |
| description | The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA. |
| format | Article |
| id | doaj-art-503e4dc8719846a0ad0159adb9d7eb74 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-503e4dc8719846a0ad0159adb9d7eb742025-02-03T05:44:22ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/380948380948The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood PhagocytesBarbora Safrankova0Silvie Gajdova1Lukas Kubala2Institute of Biophysics, The Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65 Brno, Czech RepublicInstitute of Biophysics, The Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65 Brno, Czech RepublicInstitute of Biophysics, The Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65 Brno, Czech RepublicThe regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA.http://dx.doi.org/10.1155/2010/380948 |
| spellingShingle | Barbora Safrankova Silvie Gajdova Lukas Kubala The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes Mediators of Inflammation |
| title | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
| title_full | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
| title_fullStr | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
| title_full_unstemmed | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
| title_short | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
| title_sort | potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes |
| url | http://dx.doi.org/10.1155/2010/380948 |
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