Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic developmen...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001142 |
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| author | Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo |
| author_facet | Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo |
| author_sort | Qun Huang |
| collection | DOAJ |
| description | Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as “ion transport regulation”, “immune response” and “cell cycle”. Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport. |
| format | Article |
| id | doaj-art-503adf1b3ef2425896c87e42aed58f64 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-503adf1b3ef2425896c87e42aed58f642025-02-02T23:24:38ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112383392Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membraneQun Huang0Ran Yang1Qia Wang2Hui Teng3Hongbo Song4Fang Geng5Peng Luo6School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan 610106, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMeat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan 610106, China; Corresponding author.School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China; Corresponding author.Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as “ion transport regulation”, “immune response” and “cell cycle”. Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport.http://www.sciencedirect.com/science/article/pii/S2213453021001142IncubationChorioallantoic membraneTranscriptomeCalcium transport |
| spellingShingle | Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane Food Science and Human Wellness Incubation Chorioallantoic membrane Transcriptome Calcium transport |
| title | Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| title_full | Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| title_fullStr | Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| title_full_unstemmed | Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| title_short | Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| title_sort | transcriptome based insights into the calcium transport mechanism of chick chorioallantoic membrane |
| topic | Incubation Chorioallantoic membrane Transcriptome Calcium transport |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001142 |
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