3D bioprinting of a perfusable skin-on-chip model suitable for drug testing and wound healing studies
The skin, as the body's largest organ, serves critical functions including physical protection, thermoregulation, sensation, and immunity, making it a key focus in tissue engineering. Recently, 3D bioprinting has emerged as a promising method for fabricating skin substitutes, offering potential...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425005447 |
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| Summary: | The skin, as the body's largest organ, serves critical functions including physical protection, thermoregulation, sensation, and immunity, making it a key focus in tissue engineering. Recently, 3D bioprinting has emerged as a promising method for fabricating skin substitutes, offering potential applications in both drug testing and clinical treatments for severe skin injuries. This technology enables the precise deposition of cells within a biomaterial matrix to create complex tissue structures with controlled microenvironments. A major challenge in 3D bioprinted skin models is incorporating a vascular system for adequate nutrient and oxygen distribution. Here, we present a novel approach for creating a perfusable 3D vascularized skin model using two bioinks: gelatin methacryloyl (GelMA) for the dermal and epidermal layers and Pluronic F127 as a sacrificial material for vascular channel formation. This method integrates three cell types, neonatal foreskin fibroblasts, human epidermal keratinocytes, and human umbilical vein endothelial cells, to establish a biomimetic skin construct. By employing sacrificial bioprinting techniques, we successfully developed a skin model with vascularized structures that can be used for advanced in-vitro studies and regenerative therapies. |
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| ISSN: | 2590-0064 |