Heme alters biofilm formation in Mycobacterium abscessus
ABSTRACT Mycobacterium abscessus (Mabs) is commonly found in the cystic fibrosis (CF) lung. During infection, Mabs can form biofilms in the lung which reduce both the ability of the immune response to clear infection and the effectiveness of antibiotic therapy. In the CF lung, heme and hemoglobin le...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02415-24 |
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| author | Hadia Aftab Jessica Samudio Grace Wang Lily Le Rajesh K. Soni Rebecca K. Donegan |
| author_facet | Hadia Aftab Jessica Samudio Grace Wang Lily Le Rajesh K. Soni Rebecca K. Donegan |
| author_sort | Hadia Aftab |
| collection | DOAJ |
| description | ABSTRACT Mycobacterium abscessus (Mabs) is commonly found in the cystic fibrosis (CF) lung. During infection, Mabs can form biofilms in the lung which reduce both the ability of the immune response to clear infection and the effectiveness of antibiotic therapy. In the CF lung, heme and hemoglobin levels are increased and may provide both iron and heme to Mabs cells. In this work, we show that exogenous heme altered Mabs biofilm formation and measured the effects of exogenous heme on protein level and metabolism in Mabs. Our findings suggest that heme impacts iron homeostasis in Mabs and affects other aspects of its metabolism, highlighting the potential role of heme as a critical nutrient for Mabs growth and biofilm formation.IMPORTANCEMycobacterium abscessus (Mabs) is commonly found in the cystic fibrosis (CF) lung, where Mabs can form biofilms that can reduce the efficacy of antibiotics. During infection, the CF lung can have more than 10 times the extracellular heme than that of a healthy lung. We have found that extracellular heme can change the way Mabs cells grow and form biofilms, which may have implications for pathogenesis. |
| format | Article |
| id | doaj-art-50150099be6d4bc9a9a708fb763b5d86 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-50150099be6d4bc9a9a708fb763b5d862025-02-04T14:03:40ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.02415-24Heme alters biofilm formation in Mycobacterium abscessusHadia Aftab0Jessica Samudio1Grace Wang2Lily Le3Rajesh K. Soni4Rebecca K. Donegan5Department of Chemistry, Barnard College, Columbia University, New York, New York, USADepartment of Chemistry, Barnard College, Columbia University, New York, New York, USADepartment of Chemistry, Barnard College, Columbia University, New York, New York, USADepartment of Chemistry, Barnard College, Columbia University, New York, New York, USAProteomics and Macromolecular Crystallography Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, New York, USADepartment of Chemistry, Barnard College, Columbia University, New York, New York, USAABSTRACT Mycobacterium abscessus (Mabs) is commonly found in the cystic fibrosis (CF) lung. During infection, Mabs can form biofilms in the lung which reduce both the ability of the immune response to clear infection and the effectiveness of antibiotic therapy. In the CF lung, heme and hemoglobin levels are increased and may provide both iron and heme to Mabs cells. In this work, we show that exogenous heme altered Mabs biofilm formation and measured the effects of exogenous heme on protein level and metabolism in Mabs. Our findings suggest that heme impacts iron homeostasis in Mabs and affects other aspects of its metabolism, highlighting the potential role of heme as a critical nutrient for Mabs growth and biofilm formation.IMPORTANCEMycobacterium abscessus (Mabs) is commonly found in the cystic fibrosis (CF) lung, where Mabs can form biofilms that can reduce the efficacy of antibiotics. During infection, the CF lung can have more than 10 times the extracellular heme than that of a healthy lung. We have found that extracellular heme can change the way Mabs cells grow and form biofilms, which may have implications for pathogenesis.https://journals.asm.org/doi/10.1128/spectrum.02415-24Mycobacterium abscessusheme utilizationbiofilmheme homeostasisheme as a nutrient |
| spellingShingle | Hadia Aftab Jessica Samudio Grace Wang Lily Le Rajesh K. Soni Rebecca K. Donegan Heme alters biofilm formation in Mycobacterium abscessus Microbiology Spectrum Mycobacterium abscessus heme utilization biofilm heme homeostasis heme as a nutrient |
| title | Heme alters biofilm formation in Mycobacterium abscessus |
| title_full | Heme alters biofilm formation in Mycobacterium abscessus |
| title_fullStr | Heme alters biofilm formation in Mycobacterium abscessus |
| title_full_unstemmed | Heme alters biofilm formation in Mycobacterium abscessus |
| title_short | Heme alters biofilm formation in Mycobacterium abscessus |
| title_sort | heme alters biofilm formation in mycobacterium abscessus |
| topic | Mycobacterium abscessus heme utilization biofilm heme homeostasis heme as a nutrient |
| url | https://journals.asm.org/doi/10.1128/spectrum.02415-24 |
| work_keys_str_mv | AT hadiaaftab hemealtersbiofilmformationinmycobacteriumabscessus AT jessicasamudio hemealtersbiofilmformationinmycobacteriumabscessus AT gracewang hemealtersbiofilmformationinmycobacteriumabscessus AT lilyle hemealtersbiofilmformationinmycobacteriumabscessus AT rajeshksoni hemealtersbiofilmformationinmycobacteriumabscessus AT rebeccakdonegan hemealtersbiofilmformationinmycobacteriumabscessus |