Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture
Pericytes are periendothelial cells of the microcirculation which contribute to tissue homeostasis and hemostasis by regulating microvascular morphogenesis and stability. Because of their multipotential ex vivo differentiation capabilities, pericytes are becoming very interesting in regenerative med...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/943671 |
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| author | Silvia Cantoni Francesca Bianchi Margherita Galletti Elena Olivi Francesco Alviano Nazzareno Galiè Carlo Ventura |
| author_facet | Silvia Cantoni Francesca Bianchi Margherita Galletti Elena Olivi Francesco Alviano Nazzareno Galiè Carlo Ventura |
| author_sort | Silvia Cantoni |
| collection | DOAJ |
| description | Pericytes are periendothelial cells of the microcirculation which contribute to tissue homeostasis and hemostasis by regulating microvascular morphogenesis and stability. Because of their multipotential ex vivo differentiation capabilities, pericytes are becoming very interesting in regenerative medicine field. Several studies address this issue by attempting to isolate pericyte/mesenchymal-like cells from peripheral blood; however the origin of these cells and their culture conditions are still debated. Here we showed that early Endothelial Progenitor Cells (EPCs) expressing CD45+/CD146+/CD31+ can be a source of cells with pericyte/mesenchymal phenotype and function, identified as human Progenitor Perivascular Cells (hPPCs). We provided evidence that hPPCs have an immunophenotype consistent with Mesenchymal Stem Cells (MSCs) from human adipose tissue (hASCs) and fetal membranes of term placenta (FM-hMSCs). In addition, hPPCs can be subcultured and exhibit expression of pluripotent genes (OCT-4, KLF-4, and NANOG) as well as a remarkable vasculogenic potential. Our findings could be helpful to develop innovative cell-based therapies for future clinical applications with distinct therapeutic purposes. |
| format | Article |
| id | doaj-art-4fd34d326e7544e89a7079113bbd7680 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4fd34d326e7544e89a7079113bbd76802025-02-03T06:12:20ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/943671943671Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell CultureSilvia Cantoni0Francesca Bianchi1Margherita Galletti2Elena Olivi3Francesco Alviano4Nazzareno Galiè5Carlo Ventura6National Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyNational Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyNational Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyNational Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyNational Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyNational Institute of Biostructures and Biosystems (NIBB), University of Bologna, 40138 Bologna, ItalyPericytes are periendothelial cells of the microcirculation which contribute to tissue homeostasis and hemostasis by regulating microvascular morphogenesis and stability. Because of their multipotential ex vivo differentiation capabilities, pericytes are becoming very interesting in regenerative medicine field. Several studies address this issue by attempting to isolate pericyte/mesenchymal-like cells from peripheral blood; however the origin of these cells and their culture conditions are still debated. Here we showed that early Endothelial Progenitor Cells (EPCs) expressing CD45+/CD146+/CD31+ can be a source of cells with pericyte/mesenchymal phenotype and function, identified as human Progenitor Perivascular Cells (hPPCs). We provided evidence that hPPCs have an immunophenotype consistent with Mesenchymal Stem Cells (MSCs) from human adipose tissue (hASCs) and fetal membranes of term placenta (FM-hMSCs). In addition, hPPCs can be subcultured and exhibit expression of pluripotent genes (OCT-4, KLF-4, and NANOG) as well as a remarkable vasculogenic potential. Our findings could be helpful to develop innovative cell-based therapies for future clinical applications with distinct therapeutic purposes.http://dx.doi.org/10.1155/2015/943671 |
| spellingShingle | Silvia Cantoni Francesca Bianchi Margherita Galletti Elena Olivi Francesco Alviano Nazzareno Galiè Carlo Ventura Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture Stem Cells International |
| title | Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture |
| title_full | Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture |
| title_fullStr | Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture |
| title_full_unstemmed | Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture |
| title_short | Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture |
| title_sort | occurring of in vitro functional vasculogenic pericytes from human circulating early endothelial precursor cell culture |
| url | http://dx.doi.org/10.1155/2015/943671 |
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