Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice
Isolation of hepatocytes and their culture in vitro represent important avenues to explore the function of such cells. However, these studies are often difficult to perform because of the inability of hepatocytes to proliferate in vitro. Immortalization of isolated hepatocytes is thus an important s...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5129526 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561633235304448 |
|---|---|
| author | Masahiro Sato Issei Saitoh Emi Inada Shingo Nakamura Satoshi Watanabe |
| author_facet | Masahiro Sato Issei Saitoh Emi Inada Shingo Nakamura Satoshi Watanabe |
| author_sort | Masahiro Sato |
| collection | DOAJ |
| description | Isolation of hepatocytes and their culture in vitro represent important avenues to explore the function of such cells. However, these studies are often difficult to perform because of the inability of hepatocytes to proliferate in vitro. Immortalization of isolated hepatocytes is thus an important step toward continuous in vitro culture. For cellular immortalization, integration of relevant genes into the host chromosomes is a prerequisite. Transposons, which are mobile genetic elements, are known to facilitate integration of genes of interest (GOI) into chromosomes in vitro and in vivo. Here, we proposed that a combination of transposon- and liver-directed introduction of nucleic acids may confer acquisition of unlimited cellular proliferative potential on hepatocytes, enabling the possible isolation of immortalized hepatocyte cell lines, which has often failed using more traditional immortalization methods. |
| format | Article |
| id | doaj-art-4fcaf35fc82b4aae884b385138d1797c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4fcaf35fc82b4aae884b385138d1797c2025-02-03T01:24:32ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/51295265129526Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in MiceMasahiro Sato0Issei Saitoh1Emi Inada2Shingo Nakamura3Satoshi Watanabe4Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890-8544, JapanDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, JapanDepartment of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, JapanDivision of Biomedical Engineering, National Defense Medical College Research Institute, Saitama 359-8513, JapanAnimal Genome Unit, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0901, JapanIsolation of hepatocytes and their culture in vitro represent important avenues to explore the function of such cells. However, these studies are often difficult to perform because of the inability of hepatocytes to proliferate in vitro. Immortalization of isolated hepatocytes is thus an important step toward continuous in vitro culture. For cellular immortalization, integration of relevant genes into the host chromosomes is a prerequisite. Transposons, which are mobile genetic elements, are known to facilitate integration of genes of interest (GOI) into chromosomes in vitro and in vivo. Here, we proposed that a combination of transposon- and liver-directed introduction of nucleic acids may confer acquisition of unlimited cellular proliferative potential on hepatocytes, enabling the possible isolation of immortalized hepatocyte cell lines, which has often failed using more traditional immortalization methods.http://dx.doi.org/10.1155/2019/5129526 |
| spellingShingle | Masahiro Sato Issei Saitoh Emi Inada Shingo Nakamura Satoshi Watanabe Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice Stem Cells International |
| title | Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice |
| title_full | Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice |
| title_fullStr | Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice |
| title_full_unstemmed | Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice |
| title_short | Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice |
| title_sort | potential for isolation of immortalized hepatocyte cell lines by liver directed in vivo gene delivery of transposons in mice |
| url | http://dx.doi.org/10.1155/2019/5129526 |
| work_keys_str_mv | AT masahirosato potentialforisolationofimmortalizedhepatocytecelllinesbyliverdirectedinvivogenedeliveryoftransposonsinmice AT isseisaitoh potentialforisolationofimmortalizedhepatocytecelllinesbyliverdirectedinvivogenedeliveryoftransposonsinmice AT emiinada potentialforisolationofimmortalizedhepatocytecelllinesbyliverdirectedinvivogenedeliveryoftransposonsinmice AT shingonakamura potentialforisolationofimmortalizedhepatocytecelllinesbyliverdirectedinvivogenedeliveryoftransposonsinmice AT satoshiwatanabe potentialforisolationofimmortalizedhepatocytecelllinesbyliverdirectedinvivogenedeliveryoftransposonsinmice |