A survey of the Sli gene in wild and cultivated potato
Abstract Inbred‐hybrid breeding of diploid potatoes necessitates breeding lines that are self‐compatible. One way of incorporating self‐compatibility into incompatible cultivated potato (Solanum tuberosum) germplasm is to introduce the S‐locus inhibitor gene (Sli), which functions as a dominant inhi...
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| Format: | Article |
| Language: | English |
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Wiley
2024-05-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.589 |
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| author | Mercedes Ames Andy Hamernik William Behling David S. Douches Dennis A. Halterman Paul C. Bethke |
| author_facet | Mercedes Ames Andy Hamernik William Behling David S. Douches Dennis A. Halterman Paul C. Bethke |
| author_sort | Mercedes Ames |
| collection | DOAJ |
| description | Abstract Inbred‐hybrid breeding of diploid potatoes necessitates breeding lines that are self‐compatible. One way of incorporating self‐compatibility into incompatible cultivated potato (Solanum tuberosum) germplasm is to introduce the S‐locus inhibitor gene (Sli), which functions as a dominant inhibitor of gametophytic self‐incompatibility. To learn more about Sli diversity and function in wild species relatives of cultivated potato, we obtained Sli gene sequences that extended from the 5′UTR to the 3′UTR from 133 individuals from 22 wild species relatives of potato and eight diverse cultivated potato clones. DNA sequence alignment and phylogenetic trees based on genomic and protein sequences show that there are two highly conserved groups of Sli sequences. DNA sequences in one group contain the 533 bp insertion upstream of the start codon identified previously in self‐compatible potato. The second group lacks the insertion. Three diploid and four polyploid individuals of wild species collected from geographically disjointed localities contained Sli with the 533 bp insertion. For most of the wild species clones examined, however, Sli did not have the insertion. Phylogenetic analysis indicated that Sli sequences with the insertion, in wild species and in cultivated clones, trace back to a single origin. Some diploid wild potatoes that have Sli with the insertion were self‐incompatible and some wild potatoes that lack the insertion were self‐compatible. Although there is evidence of positive selection for some codon positions in Sli, there is no evidence of diversifying selection at the gene level. In silico analysis of Sli protein structure did not support the hypothesis that amino acid changes from wild‐type (no insertion) to insertion‐type account for changes in protein function. Our study demonstrated that genetic factors besides the Sli gene must be important for conditioning a switch in the mating system from self‐incompatible to self‐compatible in wild potatoes. |
| format | Article |
| id | doaj-art-4f83a1a22fd242d49b48a8c080595bc4 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-4f83a1a22fd242d49b48a8c080595bc42025-02-04T08:30:58ZengWileyPlant Direct2475-44552024-05-0185n/an/a10.1002/pld3.589A survey of the Sli gene in wild and cultivated potatoMercedes Ames0Andy Hamernik1William Behling2David S. Douches3Dennis A. Halterman4Paul C. Bethke5US Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture University of Wisconsin Madison Wisconsin USAUS Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture University of Wisconsin Madison Wisconsin USADepartment of Plant, Soil and Microbial Sciences Michigan State University East Lansing Michigan USADepartment of Plant, Soil and Microbial Sciences Michigan State University East Lansing Michigan USAUS Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture University of Wisconsin Madison Wisconsin USAUS Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture University of Wisconsin Madison Wisconsin USAAbstract Inbred‐hybrid breeding of diploid potatoes necessitates breeding lines that are self‐compatible. One way of incorporating self‐compatibility into incompatible cultivated potato (Solanum tuberosum) germplasm is to introduce the S‐locus inhibitor gene (Sli), which functions as a dominant inhibitor of gametophytic self‐incompatibility. To learn more about Sli diversity and function in wild species relatives of cultivated potato, we obtained Sli gene sequences that extended from the 5′UTR to the 3′UTR from 133 individuals from 22 wild species relatives of potato and eight diverse cultivated potato clones. DNA sequence alignment and phylogenetic trees based on genomic and protein sequences show that there are two highly conserved groups of Sli sequences. DNA sequences in one group contain the 533 bp insertion upstream of the start codon identified previously in self‐compatible potato. The second group lacks the insertion. Three diploid and four polyploid individuals of wild species collected from geographically disjointed localities contained Sli with the 533 bp insertion. For most of the wild species clones examined, however, Sli did not have the insertion. Phylogenetic analysis indicated that Sli sequences with the insertion, in wild species and in cultivated clones, trace back to a single origin. Some diploid wild potatoes that have Sli with the insertion were self‐incompatible and some wild potatoes that lack the insertion were self‐compatible. Although there is evidence of positive selection for some codon positions in Sli, there is no evidence of diversifying selection at the gene level. In silico analysis of Sli protein structure did not support the hypothesis that amino acid changes from wild‐type (no insertion) to insertion‐type account for changes in protein function. Our study demonstrated that genetic factors besides the Sli gene must be important for conditioning a switch in the mating system from self‐incompatible to self‐compatible in wild potatoes.https://doi.org/10.1002/pld3.589diploid potato breedingself‐compatibility locusS‐locus inhibitor protein modelingwild species germplasm |
| spellingShingle | Mercedes Ames Andy Hamernik William Behling David S. Douches Dennis A. Halterman Paul C. Bethke A survey of the Sli gene in wild and cultivated potato Plant Direct diploid potato breeding self‐compatibility locus S‐locus inhibitor protein modeling wild species germplasm |
| title | A survey of the Sli gene in wild and cultivated potato |
| title_full | A survey of the Sli gene in wild and cultivated potato |
| title_fullStr | A survey of the Sli gene in wild and cultivated potato |
| title_full_unstemmed | A survey of the Sli gene in wild and cultivated potato |
| title_short | A survey of the Sli gene in wild and cultivated potato |
| title_sort | survey of the sli gene in wild and cultivated potato |
| topic | diploid potato breeding self‐compatibility locus S‐locus inhibitor protein modeling wild species germplasm |
| url | https://doi.org/10.1002/pld3.589 |
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