Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal
Giardia duodenalis is the most prevalent intestinal protozoan infection especially in children. In Portugal scarce data are available relative to this infection in preschoolers. The present study was conducted from April to July 2009 in public preschools in Lisbon enrolling 316 children. Stool exam...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2013/252971 |
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| author | Filipa Santana Ferreira Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino Ana Maria Teixeira Duarte Cancela da Fonseca Joana da Graça Matias Gomes Rúben Miguel Lopes Rodrigues Jorge Luís Marques da Silva Atouguia Sónia Chavarria Alves Ferreira Centeno-Lima |
| author_facet | Filipa Santana Ferreira Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino Ana Maria Teixeira Duarte Cancela da Fonseca Joana da Graça Matias Gomes Rúben Miguel Lopes Rodrigues Jorge Luís Marques da Silva Atouguia Sónia Chavarria Alves Ferreira Centeno-Lima |
| author_sort | Filipa Santana Ferreira |
| collection | DOAJ |
| description | Giardia duodenalis is the most prevalent intestinal protozoan infection especially in children. In Portugal scarce data are available relative to this infection in preschoolers.
The present study was conducted from April to July 2009 in public preschools in Lisbon enrolling 316 children. Stool examination was performed through microscopy. Molecular analysis was conducted in all positive samples for G. duodenalis in order to determine the assemblage and subassemblage of this parasite. Eight of the preschoolers studied children (2.5%, 8/316) were infected with G. duodenalis. Additionally the brother of one of the infected children was also infected. Genotyping analysis targeting ssu-rRNA and β-giardin loci revealed six infections with assemblage A and 3 with assemblage B. Sub-assemblage determination was possible in four of the samples, with three A2 and one A3.
The limited number of cases precluded an association of a determined symptom with an assemblage. The data presented here show the relevance of considering G. duodenalis analysis in children with intestinal complaints even in developed countries. |
| format | Article |
| id | doaj-art-4f6de4d943464a3ba6b34518a2416bbf |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-4f6de4d943464a3ba6b34518a2416bbf2025-02-03T01:09:34ZengWileyJournal of Parasitology Research2090-00232090-00312013-01-01201310.1155/2013/252971252971Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, PortugalFilipa Santana Ferreira0Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira1Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino2Ana Maria Teixeira Duarte Cancela da Fonseca3Joana da Graça Matias Gomes4Rúben Miguel Lopes Rodrigues5Jorge Luís Marques da Silva Atouguia6Sónia Chavarria Alves Ferreira Centeno-Lima7Unidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalDepartamento de Pediatria Médica, Hospital Dona Estefânia, Rua Jacinta Marto, 1169-045 Lisboa, PortugalDepartamento de Pediatria Médica, Hospital Dona Estefânia, Rua Jacinta Marto, 1169-045 Lisboa, PortugalUnidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalUnidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalUnidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalUnidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalUnidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, PortugalGiardia duodenalis is the most prevalent intestinal protozoan infection especially in children. In Portugal scarce data are available relative to this infection in preschoolers. The present study was conducted from April to July 2009 in public preschools in Lisbon enrolling 316 children. Stool examination was performed through microscopy. Molecular analysis was conducted in all positive samples for G. duodenalis in order to determine the assemblage and subassemblage of this parasite. Eight of the preschoolers studied children (2.5%, 8/316) were infected with G. duodenalis. Additionally the brother of one of the infected children was also infected. Genotyping analysis targeting ssu-rRNA and β-giardin loci revealed six infections with assemblage A and 3 with assemblage B. Sub-assemblage determination was possible in four of the samples, with three A2 and one A3. The limited number of cases precluded an association of a determined symptom with an assemblage. The data presented here show the relevance of considering G. duodenalis analysis in children with intestinal complaints even in developed countries.http://dx.doi.org/10.1155/2013/252971 |
| spellingShingle | Filipa Santana Ferreira Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino Ana Maria Teixeira Duarte Cancela da Fonseca Joana da Graça Matias Gomes Rúben Miguel Lopes Rodrigues Jorge Luís Marques da Silva Atouguia Sónia Chavarria Alves Ferreira Centeno-Lima Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal Journal of Parasitology Research |
| title | Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal |
| title_full | Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal |
| title_fullStr | Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal |
| title_full_unstemmed | Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal |
| title_short | Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal |
| title_sort | molecular and clinical characterization of giardia duodenalis infection in preschool children from lisbon portugal |
| url | http://dx.doi.org/10.1155/2013/252971 |
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