The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation
The observed effects after ozone exposure strongly depend on ozone concentration and exposure time. We hypothesized that depending on the O3 exposure protocol, mainly either an oxidant damage or an inflammation will determine the O3 toxicity. We compared two different ozone exposure protocols: an ac...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1997-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791497 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545573112119296 |
|---|---|
| author | H. J. M. Van Hoof F. J. Zijlstra H-P. Voss I. M. Garrelds J. A. M. A. Dormans L. Van Bree A. Bast |
| author_facet | H. J. M. Van Hoof F. J. Zijlstra H-P. Voss I. M. Garrelds J. A. M. A. Dormans L. Van Bree A. Bast |
| author_sort | H. J. M. Van Hoof |
| collection | DOAJ |
| description | The observed effects after ozone exposure strongly depend on ozone concentration and exposure time. We hypothesized that depending on the O3 exposure protocol, mainly either an oxidant damage or an inflammation will determine the O3 toxicity. We compared two different ozone exposure protocols: an acute exposure (3 ppm 2 h) for studying the oxidant damage and an exposure (1 ppm 12 h) where an inflammatory component is also probably involved. We measured LDH activity and protein and albumin exudation as markers for cellular damage. After the acute exposure an increase in LDH activity was measured and after exposure to 1 ppm ozone for 12 h the exudation of protein and albumin was also enhanced. The histological examinations showed a neutrophilic inflammatory response only after exposure to 1 ppm ozone for 12 h. The acute exposure protocol resulted in an increased release of PGE2, PGD2, PGF2α and 6-ketoPGF1α whereas exposure to 1 ppm ozone for 12 h led to an additional release of LTB4. No effects were measured on the release of TxB2 and LTC4/D4/E4. These changed amounts of eicosanoids will probably contribute to the ozone-induced lung function changes. |
| format | Article |
| id | doaj-art-4f49e26d3e51409ba4279258a9067483 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-4f49e26d3e51409ba4279258a90674832025-02-03T07:25:21ZengWileyMediators of Inflammation0962-93511466-18611997-01-0165-635536110.1080/09629359791497The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammationH. J. M. Van Hoof0F. J. Zijlstra1H-P. Voss2I. M. Garrelds3J. A. M. A. Dormans4L. Van Bree5A. Bast6Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsDepartment of Pharmacology, Faculty of Medicine, Erasmus University, P.O. Box 1738, Rotterdam 3000 DR, The NetherlandsLeiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsDepartment of Pharmacology, Faculty of Medicine, Erasmus University, P.O. Box 1738, Rotterdam 3000 DR, The NetherlandsLaboratory of Pathology and Immunobiology, National Institute of Public Health and the Environment, P.O. Box 1, Bilthoven 3720 BA, The NetherlandsDepartment of Toxic Effects, Laboratory of Health Effects Research, National Institute of Public Health and the Environment, P.O. Box 1, Bilthoven 3720 BA, The NetherlandsLeiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsThe observed effects after ozone exposure strongly depend on ozone concentration and exposure time. We hypothesized that depending on the O3 exposure protocol, mainly either an oxidant damage or an inflammation will determine the O3 toxicity. We compared two different ozone exposure protocols: an acute exposure (3 ppm 2 h) for studying the oxidant damage and an exposure (1 ppm 12 h) where an inflammatory component is also probably involved. We measured LDH activity and protein and albumin exudation as markers for cellular damage. After the acute exposure an increase in LDH activity was measured and after exposure to 1 ppm ozone for 12 h the exudation of protein and albumin was also enhanced. The histological examinations showed a neutrophilic inflammatory response only after exposure to 1 ppm ozone for 12 h. The acute exposure protocol resulted in an increased release of PGE2, PGD2, PGF2α and 6-ketoPGF1α whereas exposure to 1 ppm ozone for 12 h led to an additional release of LTB4. No effects were measured on the release of TxB2 and LTC4/D4/E4. These changed amounts of eicosanoids will probably contribute to the ozone-induced lung function changes.http://dx.doi.org/10.1080/09629359791497 |
| spellingShingle | H. J. M. Van Hoof F. J. Zijlstra H-P. Voss I. M. Garrelds J. A. M. A. Dormans L. Van Bree A. Bast The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation Mediators of Inflammation |
| title | The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation |
| title_full | The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation |
| title_fullStr | The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation |
| title_full_unstemmed | The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation |
| title_short | The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation |
| title_sort | effect of ozone exposure on the release of eicosanoids in guinea pig bal fluid in relation to cellular damage and inflammation |
| url | http://dx.doi.org/10.1080/09629359791497 |
| work_keys_str_mv | AT hjmvanhoof theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT fjzijlstra theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT hpvoss theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT imgarrelds theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT jamadormans theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT lvanbree theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT abast theeffectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT hjmvanhoof effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT fjzijlstra effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT hpvoss effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT imgarrelds effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT jamadormans effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT lvanbree effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation AT abast effectofozoneexposureonthereleaseofeicosanoidsinguineapigbalfluidinrelationtocellulardamageandinflammation |