Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
Aim. This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. Methods. Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmen...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8303450 |
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| author | Fabiano Luiz Heggendorn Aline Guerra Manssour Fraga Dennis de Carvalho Ferreira Lucio Souza Gonçalves Viviane de Oliveira Freitas Lione Márcia Teresa Soares Lutterbach |
| author_facet | Fabiano Luiz Heggendorn Aline Guerra Manssour Fraga Dennis de Carvalho Ferreira Lucio Souza Gonçalves Viviane de Oliveira Freitas Lione Márcia Teresa Soares Lutterbach |
| author_sort | Fabiano Luiz Heggendorn |
| collection | DOAJ |
| description | Aim. This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. Methods. Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). Results. The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. Conclusion. The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels. |
| format | Article |
| id | doaj-art-4f41d777493142ee8e8df37b81f95a6a |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-4f41d777493142ee8e8df37b81f95a6a2025-02-03T01:31:12ZengWileyInternational Journal of Dentistry1687-87281687-87362018-01-01201810.1155/2018/83034508303450Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic FilesFabiano Luiz Heggendorn0Aline Guerra Manssour Fraga1Dennis de Carvalho Ferreira2Lucio Souza Gonçalves3Viviane de Oliveira Freitas Lione4Márcia Teresa Soares Lutterbach5School of Pharmacy, Federal University of Rio de Janeiro Pharmaceutical Laboratory Bioassays, Rio de Janeiro, RJ, BrazilLaboratory of Organic Synthesis and Medical Chemistry, School of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilFaculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, BrazilFaculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, BrazilSchool of Pharmacy, Federal University of Rio de Janeiro Pharmaceutical Laboratory Bioassays, Rio de Janeiro, RJ, BrazilLaboratory of Biocorrosion and Biodegradation, National Institute of Technology, Rio de Janeiro, RJ, BrazilAim. This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. Methods. Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). Results. The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. Conclusion. The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels.http://dx.doi.org/10.1155/2018/8303450 |
| spellingShingle | Fabiano Luiz Heggendorn Aline Guerra Manssour Fraga Dennis de Carvalho Ferreira Lucio Souza Gonçalves Viviane de Oliveira Freitas Lione Márcia Teresa Soares Lutterbach Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files International Journal of Dentistry |
| title | Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files |
| title_full | Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files |
| title_fullStr | Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files |
| title_full_unstemmed | Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files |
| title_short | Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files |
| title_sort | sulfate reducing bacteria biofilm formation and corrosive activity in endodontic files |
| url | http://dx.doi.org/10.1155/2018/8303450 |
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