Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs
Abstract The emergence of multidrug-resistant (MDR) Raoultella isolates is linked to the acquisition of antibiotic resistance genes (ARGs) with plasmids playing a pivotal role in this process. While plasmid-mediated transmission of ARGs in Raoultella has been extensively reported, limited attention...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-025-03760-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571926958047232 |
|---|---|
| author | Jiao Feng Changxin Wu Dongsheng Zhou Lingfei Hu Kai Mu Zhe Yin |
| author_facet | Jiao Feng Changxin Wu Dongsheng Zhou Lingfei Hu Kai Mu Zhe Yin |
| author_sort | Jiao Feng |
| collection | DOAJ |
| description | Abstract The emergence of multidrug-resistant (MDR) Raoultella isolates is linked to the acquisition of antibiotic resistance genes (ARGs) with plasmids playing a pivotal role in this process. While plasmid-mediated transmission of ARGs in Raoultella has been extensively reported, limited attention has been given to genetically dissecting the modular structures of plasmids. This study aims to elucidate the genomic features of novel incompatible plasmids in MDR Raoultella by presenting 13 complete plasmid sequences from four isolates, along with an analysis of 16 related plasmids from GenBank. These 29 plasmids were classified into five distinct groups: IncFII single-replicon plasmids, dual-replicon plasmids containing the IncFII replicon, IncHI plasmids, IncR plasmids, and IncX8 plasmids. A new incompatible group, IncFIIp23141−CTXM, was identified, alongside five newly designated Inc groups based on previously determined sequences, namely IncFIIpKPC2_EC14653, IncFIIpCP020359, IncFIIpCP024509, IncFIIpKOX−137, and IncFIIpKDO1. Furthermore, this research marks the first report of four Inc groups of plasmids within Raoultella, namely IncFIIp23141−CTXM plasmid, IncFIIpKPC2_EC14653 plasmid, IncX8 plasmid, and IncFIIpCP020359: IncFIB-7.1 dual-replicon plasmid. Moreover, novel mobile genetic elements, including two unit transposons (Tn6806 and Tn6891), one IS-based transposition unit (Tn6561), and four insertion sequences (ISRor6, ISRor7, ISRor8, and ISRor9) were discovered. Notably, this is the first report of mcr-9 in clinical Raoultella strains. At least 49 ARGs conferring resistance against 11 different categories of antimicrobials were identified on these 13 plasmids. Overall, this research deepens the understanding of incompatible plasmids in Raoultella, serving as a reference for exploring antibiotic resistance profiles and plasmid diversity in MDR Raoultella. |
| format | Article |
| id | doaj-art-4f263be9fbdd4ece9e3eacf8419fa895 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-4f263be9fbdd4ece9e3eacf8419fa8952025-02-02T12:11:13ZengBMCBMC Microbiology1471-21802025-01-0125111710.1186/s12866-025-03760-8Insights into incompatible plasmids in multidrug-resistant Raoultella superbugsJiao Feng0Changxin Wu1Dongsheng Zhou2Lingfei Hu3Kai Mu4Zhe Yin5Institute of Biomedical Sciences, The Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Shanxi UniversityInstitute of Biomedical Sciences, The Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Shanxi UniversityState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesAbstract The emergence of multidrug-resistant (MDR) Raoultella isolates is linked to the acquisition of antibiotic resistance genes (ARGs) with plasmids playing a pivotal role in this process. While plasmid-mediated transmission of ARGs in Raoultella has been extensively reported, limited attention has been given to genetically dissecting the modular structures of plasmids. This study aims to elucidate the genomic features of novel incompatible plasmids in MDR Raoultella by presenting 13 complete plasmid sequences from four isolates, along with an analysis of 16 related plasmids from GenBank. These 29 plasmids were classified into five distinct groups: IncFII single-replicon plasmids, dual-replicon plasmids containing the IncFII replicon, IncHI plasmids, IncR plasmids, and IncX8 plasmids. A new incompatible group, IncFIIp23141−CTXM, was identified, alongside five newly designated Inc groups based on previously determined sequences, namely IncFIIpKPC2_EC14653, IncFIIpCP020359, IncFIIpCP024509, IncFIIpKOX−137, and IncFIIpKDO1. Furthermore, this research marks the first report of four Inc groups of plasmids within Raoultella, namely IncFIIp23141−CTXM plasmid, IncFIIpKPC2_EC14653 plasmid, IncX8 plasmid, and IncFIIpCP020359: IncFIB-7.1 dual-replicon plasmid. Moreover, novel mobile genetic elements, including two unit transposons (Tn6806 and Tn6891), one IS-based transposition unit (Tn6561), and four insertion sequences (ISRor6, ISRor7, ISRor8, and ISRor9) were discovered. Notably, this is the first report of mcr-9 in clinical Raoultella strains. At least 49 ARGs conferring resistance against 11 different categories of antimicrobials were identified on these 13 plasmids. Overall, this research deepens the understanding of incompatible plasmids in Raoultella, serving as a reference for exploring antibiotic resistance profiles and plasmid diversity in MDR Raoultella.https://doi.org/10.1186/s12866-025-03760-8RaoultellaPlasmidIncompatible groupMobile genetic elementMultidrug resistance |
| spellingShingle | Jiao Feng Changxin Wu Dongsheng Zhou Lingfei Hu Kai Mu Zhe Yin Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs BMC Microbiology Raoultella Plasmid Incompatible group Mobile genetic element Multidrug resistance |
| title | Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs |
| title_full | Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs |
| title_fullStr | Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs |
| title_full_unstemmed | Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs |
| title_short | Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs |
| title_sort | insights into incompatible plasmids in multidrug resistant raoultella superbugs |
| topic | Raoultella Plasmid Incompatible group Mobile genetic element Multidrug resistance |
| url | https://doi.org/10.1186/s12866-025-03760-8 |
| work_keys_str_mv | AT jiaofeng insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs AT changxinwu insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs AT dongshengzhou insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs AT lingfeihu insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs AT kaimu insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs AT zheyin insightsintoincompatibleplasmidsinmultidrugresistantraoultellasuperbugs |