Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina
Buffalo meat and milk production is a constantly growing activity, especially in America. Artificial insemination (AI) is a better tool with a low application in this species due to the particularities of its physiology; an alternative is the application of fixed-time insemination (FTAI). The aim o...
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Universidad del Zulia
2023-11-01
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| Online Access: | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43454 |
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| author | José Luis Konrad Alexis Bandeo Natalia Vallejos Pablo Ponce Jesús Alfredo Berdugo Pablo Maldonado-Vargas |
| author_facet | José Luis Konrad Alexis Bandeo Natalia Vallejos Pablo Ponce Jesús Alfredo Berdugo Pablo Maldonado-Vargas |
| author_sort | José Luis Konrad |
| collection | DOAJ |
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Buffalo meat and milk production is a constantly growing activity, especially in America. Artificial insemination (AI) is a better tool with a low application in this species due to the particularities of its physiology; an alternative is the application of fixed-time insemination (FTAI). The aim of the present work was to evaluate the effect of sperm source on pregnancy rates using an IATF protocol in heifers. It was performed in Argentina in four buffalo farms, during the reproductive season of 2021. A total of 627 Murrah and Mediterranean heifers were included, with age, body condition and genital development of 2.61±0.52 years, 3.58±0.58 (1-5 score), 2.49±0.50 respectively, the permeability of the cervix was also evaluated. They were synchronized using the Ovsynch protocol supplemented with 400 IU of equine chorionic gonadotrophin (eCG) on day 7. Fresh semen (30±6.5x10 6 spermatozoa) and frozen semen (40±4.4x10 6 spermatozoa) from two breeders were used. 35 days after insemination, pregnancy diagnosis was performed by ultrasonography. The data obtained were recorded in Excel spread-sheets. A comparison of gestation results was performed using the Kruskal-Wallis nonparametric test. A pregnancy rate of 50.3% (149/296) was obtained with frozen-thawed semen and 55.9% (185/331) with refrigerated semen, these differences were not statistically significant (p=0.1918). No significant differences were observed between farms (p>0.05), nor between the bulls used with the two variables of straws, with 50% and 58% of pregnancies for bull 1 and 58% and 52% and 54% of pregnancies for frozen-thawed and refrigerated semen for bull 2, respectively (p>0.05). The fertility of frozen-thawed semen was lower than that of frozen- thawed semen, without being significant, which should motivate veterinarians and breeders to cryopreserve semen from breeding stock. It is shown how acceptable pregnancy rates are obtained independently of the semen condition, which allows visualizing the application of artificial insemination in this species to achieve the necessary improvement to satisfy the needs of the industry.
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| format | Article |
| id | doaj-art-4f235064dff74198a1d2fe3de033ef5e |
| institution | Kabale University |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-4f235064dff74198a1d2fe3de033ef5e2025-02-04T15:37:01ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133Suplemento10.52973/rcfcv-wbc108Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in ArgentinaJosé Luis Konrad0Alexis Bandeo 1Natalia Vallejos 2Pablo Ponce 3Jesús Alfredo Berdugo4Pablo Maldonado-Vargas5Instituto de Biotecnología de Reproducción Animal (IBRA), Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, ArgentinaInstituto de Biotecnología de Reproducción Animal (IBRA), Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, ArgentinaInstituto de Biotecnología de Reproducción Animal (IBRA), Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, ArgentinaInstituto de Biotecnología de Reproducción Animal (IBRA), Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, ArgentinaUniversidad Nacional de Colombia, Sede Orinoquia. Arauca, ColombiaInstituto de Biotecnología de Reproducción Animal (IBRA), Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina Buffalo meat and milk production is a constantly growing activity, especially in America. Artificial insemination (AI) is a better tool with a low application in this species due to the particularities of its physiology; an alternative is the application of fixed-time insemination (FTAI). The aim of the present work was to evaluate the effect of sperm source on pregnancy rates using an IATF protocol in heifers. It was performed in Argentina in four buffalo farms, during the reproductive season of 2021. A total of 627 Murrah and Mediterranean heifers were included, with age, body condition and genital development of 2.61±0.52 years, 3.58±0.58 (1-5 score), 2.49±0.50 respectively, the permeability of the cervix was also evaluated. They were synchronized using the Ovsynch protocol supplemented with 400 IU of equine chorionic gonadotrophin (eCG) on day 7. Fresh semen (30±6.5x10 6 spermatozoa) and frozen semen (40±4.4x10 6 spermatozoa) from two breeders were used. 35 days after insemination, pregnancy diagnosis was performed by ultrasonography. The data obtained were recorded in Excel spread-sheets. A comparison of gestation results was performed using the Kruskal-Wallis nonparametric test. A pregnancy rate of 50.3% (149/296) was obtained with frozen-thawed semen and 55.9% (185/331) with refrigerated semen, these differences were not statistically significant (p=0.1918). No significant differences were observed between farms (p>0.05), nor between the bulls used with the two variables of straws, with 50% and 58% of pregnancies for bull 1 and 58% and 52% and 54% of pregnancies for frozen-thawed and refrigerated semen for bull 2, respectively (p>0.05). The fertility of frozen-thawed semen was lower than that of frozen- thawed semen, without being significant, which should motivate veterinarians and breeders to cryopreserve semen from breeding stock. It is shown how acceptable pregnancy rates are obtained independently of the semen condition, which allows visualizing the application of artificial insemination in this species to achieve the necessary improvement to satisfy the needs of the industry. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43454IATFfrozen semenfresh semenimprovement |
| spellingShingle | José Luis Konrad Alexis Bandeo Natalia Vallejos Pablo Ponce Jesús Alfredo Berdugo Pablo Maldonado-Vargas Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina Revista Científica IATF frozen semen fresh semen improvement |
| title | Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina |
| title_full | Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina |
| title_fullStr | Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina |
| title_full_unstemmed | Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina |
| title_short | Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina |
| title_sort | fixed timed artificial insemination in buffalo heifers with fresh and frozen thawed semen in argentina |
| topic | IATF frozen semen fresh semen improvement |
| url | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43454 |
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