Effects of housefly larvae meal and multi-enzyme-probiotic supplementation on performance, egg quality, and blood biochemistry in laying hens

This study investigated the effects of dietary housefly larvae meal (HFLM) and Rayabold (multi-enzyme-probiotic supplement) on the performance, egg quality, and blood biochemistry of 192 Lohmann LSL-Lite laying hens at 56 weeks of age. A 10-week feeding trial was conducted using a 4 × 2 factorial de...

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Main Authors: Abolfazl Salehizadeh, Ph.D, Mehran Torki, Ph.D, Maryam Darbemamieh, Ph.D, Seyed Davood Sharifi, Ph.D
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225005815
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Summary:This study investigated the effects of dietary housefly larvae meal (HFLM) and Rayabold (multi-enzyme-probiotic supplement) on the performance, egg quality, and blood biochemistry of 192 Lohmann LSL-Lite laying hens at 56 weeks of age. A 10-week feeding trial was conducted using a 4 × 2 factorial design, with four HFLM inclusion levels (0, 5, 10, and 15 %) and two Rayabold levels (0 and 0.05 %). The inclusion of HFLM, with or without Rayabold, significantly reduced feed intake compared to the control; however, Rayabold partially offset this reduction at the 5 % and 10 % HFLM levels. Although feed intake was reduced, egg production remained unaffected, while egg weight increased notably in hens receiving 15 % HFLM combined with Rayabold. Both 10 % and 15 % inclusion levels of HFLM improved feed conversion ratio, protein efficiency, and energy efficiency, with Rayabold further enhanced protein and energy utilization at the 15 % inclusion level. Eggshell weight, thickness, and yolk height improved significantly in the 15 % HFLM + Rayabold group. Metabolic responses included reduced blood glucose at 5 % and 10 % HFLM, and lower cholesterol at 10 %. Furthermore, triglyceride levels declined with both HFLM and Rayabold supplementation. Aspartate aminotransferase activity decreased in the 10 % and 15 % HFLM groups, while alanine aminotransferase was highest in the 15 % + Rayabold group, suggesting possible hepatic stress. Overall, 10 % inclusion of HFLM, with or without Rayabold, offers a nutritionally effective and metabolically safe strategy to enhance laying hen productivity and physiological health, supporting its potential as a sustainable alternative protein source.
ISSN:2772-5022