The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats
The aim of the current study was to investigate the protective effect of naringin on bleomycin-induced pulmonary fibrosis in rats. Twenty-four Wistar rats randomly divided into four groups (control, bleomycin alone, bleomycin + naringin 40, and bleomycin + naringin 80) were used. Rats were administe...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2016/7601393 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563769592512512 |
|---|---|
| author | Nergiz H. Turgut Haki Kara Sahende Elagoz Koksal Deveci Huseyin Gungor Emre Arslanbas |
| author_facet | Nergiz H. Turgut Haki Kara Sahende Elagoz Koksal Deveci Huseyin Gungor Emre Arslanbas |
| author_sort | Nergiz H. Turgut |
| collection | DOAJ |
| description | The aim of the current study was to investigate the protective effect of naringin on bleomycin-induced pulmonary fibrosis in rats. Twenty-four Wistar rats randomly divided into four groups (control, bleomycin alone, bleomycin + naringin 40, and bleomycin + naringin 80) were used. Rats were administered a single dose of bleomycin (5 mg/kg; via the tracheal cannula) alone or followed by either naringin 40 mg/kg (orally) or naringin 80 mg/kg (orally) or water (1 mL, orally) for 14 days. Rats and lung tissue were weighed to determine the lung index. TNF-α and IL-1β levels, hydroxyproline content, and malondialdehyde (MDA) levels were assayed. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined. Tissue sections were stained with hematoxylin-eosin, Masson’s trichrome, and 0.1% toluidine blue. TNF-α, IL-1β, and MDA levels and hydroxyproline content significantly increased (p<0.01) and GPx and SOD activities significantly decreased in bleomycin group (p<0.01). Naringin at a dose of 80 mg/kg body weight significantly decreased TNF-α and IL-1β activity, hydroxyproline content, and MDA level (p<0.01) and increased GPx and SOD activities (p<0.05). Histological evidence supported the results. These results show that naringin has the potential of reducing the toxic effects of bleomycin and may provide supportive therapy for conventional treatment methods for idiopathic pulmonary fibrosis. |
| format | Article |
| id | doaj-art-4ebf8aa3f25646ceb652ed27af21bfa8 |
| institution | Kabale University |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-4ebf8aa3f25646ceb652ed27af21bfa82025-02-03T01:12:34ZengWileyPulmonary Medicine2090-18362090-18442016-01-01201610.1155/2016/76013937601393The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar RatsNergiz H. Turgut0Haki Kara1Sahende Elagoz2Koksal Deveci3Huseyin Gungor4Emre Arslanbas5Cumhuriyet University School of Pharmacy, Department of Pharmacology, 58140 Sivas, TurkeyCumhuriyet University School of Veterinary Medicine, Department of Pharmacology and Toxicology, 58140 Sivas, TurkeyCumhuriyet University School of Medicine, Department of Pathology, 58140 Sivas, TurkeyCumhuriyet University School of Medicine, Department of Biochemistry, 58140 Sivas, TurkeyCumhuriyet University School of Veterinary Medicine, Department of Pharmacology and Toxicology, 58140 Sivas, TurkeyCumhuriyet University School of Veterinary Medicine, Department of Pharmacology and Toxicology, 58140 Sivas, TurkeyThe aim of the current study was to investigate the protective effect of naringin on bleomycin-induced pulmonary fibrosis in rats. Twenty-four Wistar rats randomly divided into four groups (control, bleomycin alone, bleomycin + naringin 40, and bleomycin + naringin 80) were used. Rats were administered a single dose of bleomycin (5 mg/kg; via the tracheal cannula) alone or followed by either naringin 40 mg/kg (orally) or naringin 80 mg/kg (orally) or water (1 mL, orally) for 14 days. Rats and lung tissue were weighed to determine the lung index. TNF-α and IL-1β levels, hydroxyproline content, and malondialdehyde (MDA) levels were assayed. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined. Tissue sections were stained with hematoxylin-eosin, Masson’s trichrome, and 0.1% toluidine blue. TNF-α, IL-1β, and MDA levels and hydroxyproline content significantly increased (p<0.01) and GPx and SOD activities significantly decreased in bleomycin group (p<0.01). Naringin at a dose of 80 mg/kg body weight significantly decreased TNF-α and IL-1β activity, hydroxyproline content, and MDA level (p<0.01) and increased GPx and SOD activities (p<0.05). Histological evidence supported the results. These results show that naringin has the potential of reducing the toxic effects of bleomycin and may provide supportive therapy for conventional treatment methods for idiopathic pulmonary fibrosis.http://dx.doi.org/10.1155/2016/7601393 |
| spellingShingle | Nergiz H. Turgut Haki Kara Sahende Elagoz Koksal Deveci Huseyin Gungor Emre Arslanbas The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats Pulmonary Medicine |
| title | The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats |
| title_full | The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats |
| title_fullStr | The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats |
| title_full_unstemmed | The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats |
| title_short | The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats |
| title_sort | protective effect of naringin against bleomycin induced pulmonary fibrosis in wistar rats |
| url | http://dx.doi.org/10.1155/2016/7601393 |
| work_keys_str_mv | AT nergizhturgut theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT hakikara theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT sahendeelagoz theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT koksaldeveci theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT huseyingungor theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT emrearslanbas theprotectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT nergizhturgut protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT hakikara protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT sahendeelagoz protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT koksaldeveci protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT huseyingungor protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats AT emrearslanbas protectiveeffectofnaringinagainstbleomycininducedpulmonaryfibrosisinwistarrats |