Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3
In recent years an increase of functional CD4+CD25+ regulatory T cells (Treg cells) has been established for patients with solid tumors, acute leukemias, and lymphomas. We have reported an expanded pool of CD4+CD25high Treg cells in patients with chronic lymphat...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2011/734036 |
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| author | Marc Beyer Sabine Classen Elmar Endl Matthias Kochanek Martin R. Weihrauch Svenja Debey-Pascher Percy A. Knolle Joachim L. Schultze |
| author_facet | Marc Beyer Sabine Classen Elmar Endl Matthias Kochanek Martin R. Weihrauch Svenja Debey-Pascher Percy A. Knolle Joachim L. Schultze |
| author_sort | Marc Beyer |
| collection | DOAJ |
| description | In recent years an increase of functional CD4+CD25+ regulatory T cells (Treg cells) has been established for patients with
solid tumors, acute leukemias, and lymphomas. We have reported an expanded pool of CD4+CD25high Treg cells in patients
with chronic lymphatic leukemia (CLL), multiple myeloma (MM) as well as its premalignant precursor monoclonal gammopathy of undetermined significance (MGUS). In healthy individuals, low-level expression of
CD127 on T cells in addition to the expression of FOXP3 has been associated with Treg cells. Here, we demonstrate that the expanded FOXP3+ T-cell population in
patients with colorectal cancer, CLL, MGUS, MM, follicular lymphoma, and Hodgkin's disease are exclusively CD127low Treg cells and
were strongly suppressive. A significant portion of CD127lowFOXP3+ Treg cells expressed only low levels of CD25 suggesting
that the previously reported expansion of CD25+ Treg cells underestimates the true expansion. The assessment of CCR7 and CD45RA expression on
the expanded CD4+CD127lowFOXP3+ Treg cells revealed an increase of both naïve as well as central
and effector memory Treg cells in peripheral blood. Our data strongly support superiority of combined CD127 and FOXP3 analysis in comparison to CD25 and FOXP3 assessment
for further quantification of Treg cells in malignant diseases. |
| format | Article |
| id | doaj-art-4eaa9cd671a54d299d9c3d93bb65ba84 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-4eaa9cd671a54d299d9c3d93bb65ba842025-02-03T06:07:03ZengWileyClinical and Developmental Immunology1740-25221740-25302011-01-01201110.1155/2011/734036734036Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3Marc Beyer0Sabine Classen1Elmar Endl2Matthias Kochanek3Martin R. Weihrauch4Svenja Debey-Pascher5Percy A. Knolle6Joachim L. Schultze7Laboratory for Genomics and Immunoregulation, LIMES-Institute, University of Bonn, Carl-Troll-Street 31, 53115 Bonn, GermanyLaboratory for Genomics and Immunoregulation, LIMES-Institute, University of Bonn, Carl-Troll-Street 31, 53115 Bonn, GermanyInstitute for Molecular Medicine and Experimental Immunology, University of Bonn,, 53105 Bonn, GermanyClinic I for Internal Medicine, University of Cologne, Kerpenerstr. 62, 50924 Cologn, GermanyClinic I for Internal Medicine, University of Cologne, Kerpenerstr. 62, 50924 Cologn, GermanyLaboratory for Genomics and Immunoregulation, LIMES-Institute, University of Bonn, Carl-Troll-Street 31, 53115 Bonn, GermanyInstitute for Molecular Medicine and Experimental Immunology, University of Bonn,, 53105 Bonn, GermanyClinic I for Internal Medicine, University of Cologne, Kerpenerstr. 62, 50924 Cologn, GermanyIn recent years an increase of functional CD4+CD25+ regulatory T cells (Treg cells) has been established for patients with solid tumors, acute leukemias, and lymphomas. We have reported an expanded pool of CD4+CD25high Treg cells in patients with chronic lymphatic leukemia (CLL), multiple myeloma (MM) as well as its premalignant precursor monoclonal gammopathy of undetermined significance (MGUS). In healthy individuals, low-level expression of CD127 on T cells in addition to the expression of FOXP3 has been associated with Treg cells. Here, we demonstrate that the expanded FOXP3+ T-cell population in patients with colorectal cancer, CLL, MGUS, MM, follicular lymphoma, and Hodgkin's disease are exclusively CD127low Treg cells and were strongly suppressive. A significant portion of CD127lowFOXP3+ Treg cells expressed only low levels of CD25 suggesting that the previously reported expansion of CD25+ Treg cells underestimates the true expansion. The assessment of CCR7 and CD45RA expression on the expanded CD4+CD127lowFOXP3+ Treg cells revealed an increase of both naïve as well as central and effector memory Treg cells in peripheral blood. Our data strongly support superiority of combined CD127 and FOXP3 analysis in comparison to CD25 and FOXP3 assessment for further quantification of Treg cells in malignant diseases.http://dx.doi.org/10.1155/2011/734036 |
| spellingShingle | Marc Beyer Sabine Classen Elmar Endl Matthias Kochanek Martin R. Weihrauch Svenja Debey-Pascher Percy A. Knolle Joachim L. Schultze Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 Clinical and Developmental Immunology |
| title | Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 |
| title_full | Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 |
| title_fullStr | Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 |
| title_full_unstemmed | Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 |
| title_short | Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3 |
| title_sort | comparative approach to define increased regulatory t cells in different cancer subtypes by combined assessment of cd127 and foxp3 |
| url | http://dx.doi.org/10.1155/2011/734036 |
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