Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions
Purpose. To assess the role of selected matrix metalloproteinases in defective corneal re-epithelization in patients with recurrent corneal erosions. Subjects. The study group (group 1) included patients with recurrent corneal erosions qualified for phototherapeutic keratectomy. The group 1 was divi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2022/5024037 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563288268865536 |
|---|---|
| author | Katarzyna Jadczyk-Sorek Wojciech Garczorz Beata Bubała-Stachowicz Tomasz Francuz Ewa Mrukwa-Kominek |
| author_facet | Katarzyna Jadczyk-Sorek Wojciech Garczorz Beata Bubała-Stachowicz Tomasz Francuz Ewa Mrukwa-Kominek |
| author_sort | Katarzyna Jadczyk-Sorek |
| collection | DOAJ |
| description | Purpose. To assess the role of selected matrix metalloproteinases in defective corneal re-epithelization in patients with recurrent corneal erosions. Subjects. The study group (group 1) included patients with recurrent corneal erosions qualified for phototherapeutic keratectomy. The group 1 was divided into two subgroups regarding the etiology of recurrent corneal erosions: group 1A, Cogan’s basement membrane dystrophy, and group 1B, trauma. The control group (group 2) included patients with healthy eyes qualified for Epi-Bowman Keratectomy. Methods. The analyzed material was the corneal epithelium collected during phototherapeutic keratectomy or Epi-Bowman Keratectomy in the study or control group, respectively. Matrix metalloproteinases concentration was determined by an immunohistochemical method using Human Magnetic Luminex® Assay. Results. The study revealed a statistically significantly higher concentration of matrix metalloproteinase-2 in group 1 compared to the control and a statistically significantly higher concentration of matrix metalloproteinase-3 in group 1 compared to the control. Conclusions. The results obtained in the study can prove that matrix metalloproteinase-2 and matrix metalloproteinase-3 having the ability to dissolve anchoring fibers and the corneal epithelial basement membrane could be responsible for epithelial instability and their accumulation in the corneal epithelium may induce recurrence of erosion. |
| format | Article |
| id | doaj-art-4ea91a758a36415d8b395f9e598e5b7f |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-4ea91a758a36415d8b395f9e598e5b7f2025-02-03T01:20:36ZengWileyJournal of Ophthalmology2090-00582022-01-01202210.1155/2022/5024037Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal ErosionsKatarzyna Jadczyk-Sorek0Wojciech Garczorz1Beata Bubała-Stachowicz2Tomasz Francuz3Ewa Mrukwa-Kominek4Department of OphthalmologyDepartment of BiochemistryDepartment of OphthalmologyDepartment of BiochemistryDepartment of OphthalmologyPurpose. To assess the role of selected matrix metalloproteinases in defective corneal re-epithelization in patients with recurrent corneal erosions. Subjects. The study group (group 1) included patients with recurrent corneal erosions qualified for phototherapeutic keratectomy. The group 1 was divided into two subgroups regarding the etiology of recurrent corneal erosions: group 1A, Cogan’s basement membrane dystrophy, and group 1B, trauma. The control group (group 2) included patients with healthy eyes qualified for Epi-Bowman Keratectomy. Methods. The analyzed material was the corneal epithelium collected during phototherapeutic keratectomy or Epi-Bowman Keratectomy in the study or control group, respectively. Matrix metalloproteinases concentration was determined by an immunohistochemical method using Human Magnetic Luminex® Assay. Results. The study revealed a statistically significantly higher concentration of matrix metalloproteinase-2 in group 1 compared to the control and a statistically significantly higher concentration of matrix metalloproteinase-3 in group 1 compared to the control. Conclusions. The results obtained in the study can prove that matrix metalloproteinase-2 and matrix metalloproteinase-3 having the ability to dissolve anchoring fibers and the corneal epithelial basement membrane could be responsible for epithelial instability and their accumulation in the corneal epithelium may induce recurrence of erosion.http://dx.doi.org/10.1155/2022/5024037 |
| spellingShingle | Katarzyna Jadczyk-Sorek Wojciech Garczorz Beata Bubała-Stachowicz Tomasz Francuz Ewa Mrukwa-Kominek Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions Journal of Ophthalmology |
| title | Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions |
| title_full | Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions |
| title_fullStr | Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions |
| title_full_unstemmed | Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions |
| title_short | Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions |
| title_sort | increased matrix metalloproteinase 2 and matrix metalloproteinase 3 concentrations in corneal epithelium of patients with recurrent corneal erosions |
| url | http://dx.doi.org/10.1155/2022/5024037 |
| work_keys_str_mv | AT katarzynajadczyksorek increasedmatrixmetalloproteinase2andmatrixmetalloproteinase3concentrationsincornealepitheliumofpatientswithrecurrentcornealerosions AT wojciechgarczorz increasedmatrixmetalloproteinase2andmatrixmetalloproteinase3concentrationsincornealepitheliumofpatientswithrecurrentcornealerosions AT beatabubałastachowicz increasedmatrixmetalloproteinase2andmatrixmetalloproteinase3concentrationsincornealepitheliumofpatientswithrecurrentcornealerosions AT tomaszfrancuz increasedmatrixmetalloproteinase2andmatrixmetalloproteinase3concentrationsincornealepitheliumofpatientswithrecurrentcornealerosions AT ewamrukwakominek increasedmatrixmetalloproteinase2andmatrixmetalloproteinase3concentrationsincornealepitheliumofpatientswithrecurrentcornealerosions |