Lectins from the Red Marine Algal Species Bryothamnion seaforthii and Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells
The carbohydrate-binding activity of the algal lectins from the closely related red marine algal species Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect to their cell membrane glyco-receptors. These lectins in...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2009/862162 |
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| author | Vicente P. T. Pinto Henri Debray Danuta Dus Edson H. Teixeira Taianá Maia de Oliveira Victor Alves Carneiro Alrieta H. Teixeira Gerardo C. Filho Celso S. Nagano Kyria S. Nascimento Alexandre H. Sampaio Benildo S. Cavada |
| author_facet | Vicente P. T. Pinto Henri Debray Danuta Dus Edson H. Teixeira Taianá Maia de Oliveira Victor Alves Carneiro Alrieta H. Teixeira Gerardo C. Filho Celso S. Nagano Kyria S. Nascimento Alexandre H. Sampaio Benildo S. Cavada |
| author_sort | Vicente P. T. Pinto |
| collection | DOAJ |
| description | The carbohydrate-binding activity of the algal lectins from the closely related red marine algal species Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect to their cell membrane glyco-receptors. These lectins interacted with the cells tested in a dose-dependent manner. Moreover, the fluorescence spectra of both lectins clearly differentiated the cells used as shown by FACS profiles. Furthermore, as observed by confocal microscopy, BTL and BSL bound to cell surface glycoproteins underwent intense internalization, which makes them possible tools in targeting strategies. |
| format | Article |
| id | doaj-art-4ea709b7535d4990a84447eb22bd842c |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-4ea709b7535d4990a84447eb22bd842c2025-02-03T01:10:54ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422009-01-01200910.1155/2009/862162862162Lectins from the Red Marine Algal Species Bryothamnion seaforthii and Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma CellsVicente P. T. Pinto0Henri Debray1Danuta Dus2Edson H. Teixeira3Taianá Maia de Oliveira4Victor Alves Carneiro5Alrieta H. Teixeira6Gerardo C. Filho7Celso S. Nagano8Kyria S. Nascimento9Alexandre H. Sampaio10Benildo S. Cavada11Curso de Medicina/Campus de Sobral , Universidade Federal do Ceará, Fortaleza 60020-181, BrazilLaboratoire de Chimie Biologique, UMR N. 8576 CNRS, Université des Sciences et Technologies de Lille, 59655 Lille, FranceInstitute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandCurso de Medicina/Campus de Sobral , Universidade Federal do Ceará, Fortaleza 60020-181, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilCurso de Medicina/Campus de Sobral , Universidade Federal do Ceará, Fortaleza 60020-181, BrazilCurso de Medicina/Campus de Sobral , Universidade Federal do Ceará, Fortaleza 60020-181, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilBioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 6043, BrazilThe carbohydrate-binding activity of the algal lectins from the closely related red marine algal species Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect to their cell membrane glyco-receptors. These lectins interacted with the cells tested in a dose-dependent manner. Moreover, the fluorescence spectra of both lectins clearly differentiated the cells used as shown by FACS profiles. Furthermore, as observed by confocal microscopy, BTL and BSL bound to cell surface glycoproteins underwent intense internalization, which makes them possible tools in targeting strategies.http://dx.doi.org/10.1155/2009/862162 |
| spellingShingle | Vicente P. T. Pinto Henri Debray Danuta Dus Edson H. Teixeira Taianá Maia de Oliveira Victor Alves Carneiro Alrieta H. Teixeira Gerardo C. Filho Celso S. Nagano Kyria S. Nascimento Alexandre H. Sampaio Benildo S. Cavada Lectins from the Red Marine Algal Species Bryothamnion seaforthii and Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells Advances in Pharmacological Sciences |
| title | Lectins from the Red Marine Algal Species Bryothamnion seaforthii and
Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells |
| title_full | Lectins from the Red Marine Algal Species Bryothamnion seaforthii and
Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells |
| title_fullStr | Lectins from the Red Marine Algal Species Bryothamnion seaforthii and
Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells |
| title_full_unstemmed | Lectins from the Red Marine Algal Species Bryothamnion seaforthii and
Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells |
| title_short | Lectins from the Red Marine Algal Species Bryothamnion seaforthii and
Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells |
| title_sort | lectins from the red marine algal species bryothamnion seaforthii and bryothamnion triquetrum as tools to differentiate human colon carcinoma cells |
| url | http://dx.doi.org/10.1155/2009/862162 |
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