Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models

Diabetes is a metabolic disorder of the carbohydrate pathway, resulting in high blood glucose levels, an increase in thirst as well as urination, problems in vision, weight loss, and high ketone body excretion in urine. Diabetes also affects the endocrine, neurological, and circulatory systems, rais...

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Main Authors: Bilal Hayat, Khan Mubarak Ali, Shah Syed Wadood Ali, Ghias Mehreen, Khan Adnan, Zahoor Muhammad, Ullah Riaz, Iqbal Zafar, Shah Abdul Bari
Format: Article
Language:English
Published: De Gruyter 2025-06-01
Series:Open Chemistry
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Online Access:https://doi.org/10.1515/chem-2025-0133
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author Bilal Hayat
Khan Mubarak Ali
Shah Syed Wadood Ali
Ghias Mehreen
Khan Adnan
Zahoor Muhammad
Ullah Riaz
Iqbal Zafar
Shah Abdul Bari
author_facet Bilal Hayat
Khan Mubarak Ali
Shah Syed Wadood Ali
Ghias Mehreen
Khan Adnan
Zahoor Muhammad
Ullah Riaz
Iqbal Zafar
Shah Abdul Bari
author_sort Bilal Hayat
collection DOAJ
description Diabetes is a metabolic disorder of the carbohydrate pathway, resulting in high blood glucose levels, an increase in thirst as well as urination, problems in vision, weight loss, and high ketone body excretion in urine. Diabetes also affects the endocrine, neurological, and circulatory systems, raising blood pressure and lowering high-density lipoprotein levels with subsequent increases in the low-density lipoprotein level. Helicteres isora (H. isora) is a promising medicinal plant having both anti-oxidant and anti-diabetic potentials. Herein, we investigate the protective effect of H. isora extracts (crude and fractions) on paracetamol-induced hepatorenal toxicities in animal models. The extracts were initially screened for in vivo antidiabetic potential using the alloxan-induced diabetic model. The experimental rats treated with 150 mg/kg b.w. (body weight) dose of Hi-Chl extract decreased the creatinine level to 0.48 ± 0.07 mg/dL, blood urea to 19.64 ± 1.22 mg/dL, and uric acid to 2.23 ± 0.21 mg/dL, indicating the hepatorenal protective functions of the extract. Serum glutamic pyruvic transaminase and alkaline phosphatase were also normalized by the extract to 73.10 ± 4.40 mg/dL and 174.6 ± 2.81 mg/dL, respectively. The Hi-Chl extract exhibited promising anti-diabetic potential with blood glucose normalizing effect from 494.8 ± 2.52 to 159.6 ± 2.67 to 125.6 ± 3.72 mg/dL at doses of 75 and 150 mg/kg, respectively.
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institution Kabale University
issn 2391-5420
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publishDate 2025-06-01
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spelling doaj-art-4e98e76bfbfc413c85b6cd438d42bbc42025-08-20T03:47:21ZengDe GruyterOpen Chemistry2391-54202025-06-012316910.1515/chem-2025-0133Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal modelsBilal Hayat0Khan Mubarak Ali1Shah Syed Wadood Ali2Ghias Mehreen3Khan Adnan4Zahoor Muhammad5Ullah Riaz6Iqbal Zafar7Shah Abdul Bari8Department of Biotechnology, Abdul Wali Khan University Mardan (AWKUM), Mardan, 23390, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan (AWKUM), Mardan, 23390, PakistanDepartment of Pharmacy, University of Malakand, Chakdara, Chakdara, 18800, Khyber Pakhtunkhwa, PakistanDepartment of Pharmacy, University of Malakand, Chakdara, Chakdara, 18800, Khyber Pakhtunkhwa, PakistanDepartment of Chemistry, University of Malakand, Chakdara, 18800, PakistanDepartment of Biochemistry, University of Malakand, Chakdara, Dir Lower, KPK, 18800, PakistanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Surgery, College of Medicine, King Saud University, P.O.Box 7805, Riyadh, 11472, Saudi ArabiaNatural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 08826, Republic of KoreaDiabetes is a metabolic disorder of the carbohydrate pathway, resulting in high blood glucose levels, an increase in thirst as well as urination, problems in vision, weight loss, and high ketone body excretion in urine. Diabetes also affects the endocrine, neurological, and circulatory systems, raising blood pressure and lowering high-density lipoprotein levels with subsequent increases in the low-density lipoprotein level. Helicteres isora (H. isora) is a promising medicinal plant having both anti-oxidant and anti-diabetic potentials. Herein, we investigate the protective effect of H. isora extracts (crude and fractions) on paracetamol-induced hepatorenal toxicities in animal models. The extracts were initially screened for in vivo antidiabetic potential using the alloxan-induced diabetic model. The experimental rats treated with 150 mg/kg b.w. (body weight) dose of Hi-Chl extract decreased the creatinine level to 0.48 ± 0.07 mg/dL, blood urea to 19.64 ± 1.22 mg/dL, and uric acid to 2.23 ± 0.21 mg/dL, indicating the hepatorenal protective functions of the extract. Serum glutamic pyruvic transaminase and alkaline phosphatase were also normalized by the extract to 73.10 ± 4.40 mg/dL and 174.6 ± 2.81 mg/dL, respectively. The Hi-Chl extract exhibited promising anti-diabetic potential with blood glucose normalizing effect from 494.8 ± 2.52 to 159.6 ± 2.67 to 125.6 ± 3.72 mg/dL at doses of 75 and 150 mg/kg, respectively.https://doi.org/10.1515/chem-2025-0133helicteres isorahepatotoxicitynephrotoxicitydiabetesserum profilingbiochemical analysis
spellingShingle Bilal Hayat
Khan Mubarak Ali
Shah Syed Wadood Ali
Ghias Mehreen
Khan Adnan
Zahoor Muhammad
Ullah Riaz
Iqbal Zafar
Shah Abdul Bari
Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
Open Chemistry
helicteres isora
hepatotoxicity
nephrotoxicity
diabetes
serum profiling
biochemical analysis
title Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
title_full Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
title_fullStr Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
title_full_unstemmed Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
title_short Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
title_sort protective effect of helicteres isora an efficient candidate on hepatorenal toxicity and management of diabetes in animal models
topic helicteres isora
hepatotoxicity
nephrotoxicity
diabetes
serum profiling
biochemical analysis
url https://doi.org/10.1515/chem-2025-0133
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