Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.

Type 2 Diabetes (T2D) is a highly prevalent chronic metabolic disease with strong co-morbidity with obesity and cardiovascular diseases. There is growing evidence supporting the notion that a crosstalk between mitochondria and the insulin signaling cascade could be involved in the etiology of T2D an...

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Main Authors: Josep M Mercader, Montserrat Puiggros, Ayellet V Segrè, Evarist Planet, Eleonora Sorianello, David Sebastian, Sergio Rodriguez-Cuenca, Vicent Ribas, Sílvia Bonàs-Guarch, Sorin Draghici, Chenjing Yang, Sílvia Mora, Antoni Vidal-Puig, Josée Dupuis, DIAGRAM Consortium, Jose C Florez, MITIN Consortium, Antonio Zorzano, David Torrents
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003046&type=printable
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author Josep M Mercader
Montserrat Puiggros
Ayellet V Segrè
Evarist Planet
Eleonora Sorianello
David Sebastian
Sergio Rodriguez-Cuenca
Vicent Ribas
Sílvia Bonàs-Guarch
Sorin Draghici
Chenjing Yang
Sílvia Mora
Antoni Vidal-Puig
Josée Dupuis
DIAGRAM Consortium
Jose C Florez
MITIN Consortium
Antonio Zorzano
David Torrents
author_facet Josep M Mercader
Montserrat Puiggros
Ayellet V Segrè
Evarist Planet
Eleonora Sorianello
David Sebastian
Sergio Rodriguez-Cuenca
Vicent Ribas
Sílvia Bonàs-Guarch
Sorin Draghici
Chenjing Yang
Sílvia Mora
Antoni Vidal-Puig
Josée Dupuis
DIAGRAM Consortium
Jose C Florez
MITIN Consortium
Antonio Zorzano
David Torrents
author_sort Josep M Mercader
collection DOAJ
description Type 2 Diabetes (T2D) is a highly prevalent chronic metabolic disease with strong co-morbidity with obesity and cardiovascular diseases. There is growing evidence supporting the notion that a crosstalk between mitochondria and the insulin signaling cascade could be involved in the etiology of T2D and insulin resistance. In this study we investigated the molecular basis of this crosstalk by using systems biology approaches. We combined, filtered, and interrogated different types of functional interaction data, such as direct protein-protein interactions, co-expression analyses, and metabolic and signaling dependencies. As a result, we constructed the mitochondria-insulin (MITIN) network, which highlights 286 genes as candidate functional linkers between these two systems. The results of internal gene expression analysis of three independent experimental models of mitochondria and insulin signaling perturbations further support the connecting roles of these genes. In addition, we further assessed whether these genes are involved in the etiology of T2D using the genome-wide association study meta-analysis from the DIAGRAM consortium, involving 8,130 T2D cases and 38,987 controls. We found modest enrichment of genes associated with T2D amongst our linker genes (p = 0.0549), including three already validated T2D SNPs and 15 additional SNPs, which, when combined, were collectively associated to increased fasting glucose levels according to MAGIC genome wide meta-analysis (p = 8.12×10(-5)). This study highlights the potential of combining systems biology, experimental, and genome-wide association data mining for identifying novel genes and related variants that increase vulnerability to complex diseases.
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spelling doaj-art-4e81f84e7ace4752a9d21bbe1e8592d72025-08-20T03:10:41ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-01812e100304610.1371/journal.pgen.1003046Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.Josep M MercaderMontserrat PuiggrosAyellet V SegrèEvarist PlanetEleonora SorianelloDavid SebastianSergio Rodriguez-CuencaVicent RibasSílvia Bonàs-GuarchSorin DraghiciChenjing YangSílvia MoraAntoni Vidal-PuigJosée DupuisDIAGRAM ConsortiumJose C FlorezMITIN ConsortiumAntonio ZorzanoDavid TorrentsType 2 Diabetes (T2D) is a highly prevalent chronic metabolic disease with strong co-morbidity with obesity and cardiovascular diseases. There is growing evidence supporting the notion that a crosstalk between mitochondria and the insulin signaling cascade could be involved in the etiology of T2D and insulin resistance. In this study we investigated the molecular basis of this crosstalk by using systems biology approaches. We combined, filtered, and interrogated different types of functional interaction data, such as direct protein-protein interactions, co-expression analyses, and metabolic and signaling dependencies. As a result, we constructed the mitochondria-insulin (MITIN) network, which highlights 286 genes as candidate functional linkers between these two systems. The results of internal gene expression analysis of three independent experimental models of mitochondria and insulin signaling perturbations further support the connecting roles of these genes. In addition, we further assessed whether these genes are involved in the etiology of T2D using the genome-wide association study meta-analysis from the DIAGRAM consortium, involving 8,130 T2D cases and 38,987 controls. We found modest enrichment of genes associated with T2D amongst our linker genes (p = 0.0549), including three already validated T2D SNPs and 15 additional SNPs, which, when combined, were collectively associated to increased fasting glucose levels according to MAGIC genome wide meta-analysis (p = 8.12×10(-5)). This study highlights the potential of combining systems biology, experimental, and genome-wide association data mining for identifying novel genes and related variants that increase vulnerability to complex diseases.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003046&type=printable
spellingShingle Josep M Mercader
Montserrat Puiggros
Ayellet V Segrè
Evarist Planet
Eleonora Sorianello
David Sebastian
Sergio Rodriguez-Cuenca
Vicent Ribas
Sílvia Bonàs-Guarch
Sorin Draghici
Chenjing Yang
Sílvia Mora
Antoni Vidal-Puig
Josée Dupuis
DIAGRAM Consortium
Jose C Florez
MITIN Consortium
Antonio Zorzano
David Torrents
Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
PLoS Genetics
title Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
title_full Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
title_fullStr Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
title_full_unstemmed Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
title_short Identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems.
title_sort identification of novel type 2 diabetes candidate genes involved in the crosstalk between the mitochondrial and the insulin signaling systems
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003046&type=printable
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