Role of multiplex PCR in the early diagnosis of infective endocarditis
Infective endocarditis (IE) is a serious infection of the endocardium and heart valves that necessitate early diagnosis. The conventional blood culture has lots of false-negative results besides being time consuming. Polymerase chain reaction (PCR) is a rapid diagnostic tool that helps in saving...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2021-06-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_178298_5592571b1eed156a90ae3cc0824f3493.pdf |
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| Summary: | Infective endocarditis (IE) is a serious infection of the endocardium and heart valves that
necessitate early diagnosis. The conventional blood culture has lots of false-negative results
besides being time consuming. Polymerase chain reaction (PCR) is a rapid diagnostic tool that
helps in saving the patients’ life. This study aimed to investigate the feasibility of multiplex
PCR in early diagnosis of IE compared to the conventional blood culture, and to evaluate its
impact on IE diagnosis in cases of negative blood cultures. The current study was conducted
on 30 patients admitted to the Cardiology Department, Faculty of Medicine, Ain Shams
Hospitals, Cairo, Egypt, which were diagnosed clinically as infective endocarditis according to
the modified Duke’s criteria. After processing of the patient's blood samples, the blood
cultures recorded positivity in 5 cases (16.7 %). The most common recovered bacteria were,
Staphylococcus aureus 2(6.7 %), Staphylococcus epidermedis 1(3.3 %), Enterococcus faecalis
1(3.3 %), and Escherichia coli 1(3.3 %); however, no other pathogens were isolated. On the
other hand, results of multiplex PCR showed positivity in 13 cases (43.3 %), mainly;
Staphylococcus aureus 5(16.7 %), E. faecalis 3(10 %), Staphylococcus epidermidis 2(6.7 %),
Pseudomonas aeruginosa 2(6.7 %), and E. coli 1(3.3 %). No other bacterial of fungal
pathogens were detected by multiplex PCR. Finally, the multiplex PCR assay exhibited
remarkable sensitivity and feasibility in IE diagnosis over blood culture, besides being a rapid
and accurate diagnostic assay that enhances proper treatment. |
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| ISSN: | 2537-0286 2537-0294 |