Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines
Fruit and vegetable diets rich in phenolic compounds reduce the risk of various cancers and offer multiple other health benefits due to their bioactivity and powerful antioxidant properties. However, the human health benefits of most phenolic compounds are restricted due to their limited aqueous sol...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2019/2638481 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565709686702080 |
|---|---|
| author | Rashid Ahmed Muhammad Tariq Irfan S. Ahmad Hanafy Fouly Fakhar-i-Abbas Anwarul Hasan Mosbah Kushad |
| author_facet | Rashid Ahmed Muhammad Tariq Irfan S. Ahmad Hanafy Fouly Fakhar-i-Abbas Anwarul Hasan Mosbah Kushad |
| author_sort | Rashid Ahmed |
| collection | DOAJ |
| description | Fruit and vegetable diets rich in phenolic compounds reduce the risk of various cancers and offer multiple other health benefits due to their bioactivity and powerful antioxidant properties. However, the human health benefits of most phenolic compounds are restricted due to their limited aqueous solubility, low absorption, restricted passive cellular efflux, and poor gastrointestinal stability. Nanotechnology has been used to deliver various therapeutic drugs to specific targets overcoming many of the limitations of direct treatments. This study was designed to develop poly(lactic-co-glycolic acid) (PLGA) nanoencapsulated phenolic-rich extracts from Callistemon citrinus and berberine and to evaluate their effectiveness against extremely invasive MDA-MB 231, moderately invasive MCF-10A, and minimally invasive MCF-7 breast cancers. We have achieved about 80% encapsulation of phenolics from C. citrinus. Most encapsulated nanoparticles were polygonal with particles sizes of 200 to 250 nm. Release of phenolics from encapsulation during storage was biphasic during the first week and then levelled off thereafter. Nanoencapsulated phenolics from C. citrinus extract, berberine, and combination of both enhanced their bioactivity against the three breast cancer cell lines by nearly 2-fold. Growth inhibition of cells was a linear curve relative to phenolic concentration, with a maximum inhibition of nearly 100% at 0.1 mg/ml compared to control. |
| format | Article |
| id | doaj-art-4e63f0afa2ff4cd49c8da72f912119f9 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-4e63f0afa2ff4cd49c8da72f912119f92025-02-03T01:06:53ZengWileyJournal of Food Quality0146-94281745-45572019-01-01201910.1155/2019/26384812638481Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell LinesRashid Ahmed0Muhammad Tariq1Irfan S. Ahmad2Hanafy Fouly3Fakhar-i-Abbas4Anwarul Hasan5Mosbah Kushad6Department of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur 10250, Azad Jammu and Kashmir, PakistanDepartment of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur 10250, Azad Jammu and Kashmir, PakistanMicro and Nanotechnology Laboratory, University of Illinois at Urbana Champaign, Urbana, IL, USAMicro and Nanotechnology Laboratory, University of Illinois at Urbana Champaign, Urbana, IL, USABioresource Research Centre Islamabad, Islamabad, PakistanDepartment of Mechanical and Chemical Engineering, College of Engineering, Qatar University, Doha 2713, QatarDepartment of Crop Sciences, University of Illinois at Urbana Champaign, Urbana, IL 61801, USAFruit and vegetable diets rich in phenolic compounds reduce the risk of various cancers and offer multiple other health benefits due to their bioactivity and powerful antioxidant properties. However, the human health benefits of most phenolic compounds are restricted due to their limited aqueous solubility, low absorption, restricted passive cellular efflux, and poor gastrointestinal stability. Nanotechnology has been used to deliver various therapeutic drugs to specific targets overcoming many of the limitations of direct treatments. This study was designed to develop poly(lactic-co-glycolic acid) (PLGA) nanoencapsulated phenolic-rich extracts from Callistemon citrinus and berberine and to evaluate their effectiveness against extremely invasive MDA-MB 231, moderately invasive MCF-10A, and minimally invasive MCF-7 breast cancers. We have achieved about 80% encapsulation of phenolics from C. citrinus. Most encapsulated nanoparticles were polygonal with particles sizes of 200 to 250 nm. Release of phenolics from encapsulation during storage was biphasic during the first week and then levelled off thereafter. Nanoencapsulated phenolics from C. citrinus extract, berberine, and combination of both enhanced their bioactivity against the three breast cancer cell lines by nearly 2-fold. Growth inhibition of cells was a linear curve relative to phenolic concentration, with a maximum inhibition of nearly 100% at 0.1 mg/ml compared to control.http://dx.doi.org/10.1155/2019/2638481 |
| spellingShingle | Rashid Ahmed Muhammad Tariq Irfan S. Ahmad Hanafy Fouly Fakhar-i-Abbas Anwarul Hasan Mosbah Kushad Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines Journal of Food Quality |
| title | Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines |
| title_full | Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines |
| title_fullStr | Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines |
| title_full_unstemmed | Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines |
| title_short | Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines |
| title_sort | poly lactic co glycolic acid nanoparticles loaded with callistemon citrinus phenolics exhibited anticancer properties against three breast cancer cell lines |
| url | http://dx.doi.org/10.1155/2019/2638481 |
| work_keys_str_mv | AT rashidahmed polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT muhammadtariq polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT irfansahmad polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT hanafyfouly polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT fakhariabbas polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT anwarulhasan polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines AT mosbahkushad polylacticcoglycolicacidnanoparticlesloadedwithcallistemoncitrinusphenolicsexhibitedanticancerpropertiesagainstthreebreastcancercelllines |