Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS)
An ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hy...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/3489634 |
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| author | Nam Vu-Duc Trung Nguyen-Quang Thuy Le-Minh Xuyen Nguyen-Thi Tri Manh Tran Hai Anh Vu Lan-Anh Nguyen Tien Doan-Duy Bui Van Hoi Cam-Tu Vu Dung Le-Van Lan-Anh Phung-Thi Hong-An Vu-Thi Dinh Binh Chu |
| author_facet | Nam Vu-Duc Trung Nguyen-Quang Thuy Le-Minh Xuyen Nguyen-Thi Tri Manh Tran Hai Anh Vu Lan-Anh Nguyen Tien Doan-Duy Bui Van Hoi Cam-Tu Vu Dung Le-Van Lan-Anh Phung-Thi Hong-An Vu-Thi Dinh Binh Chu |
| author_sort | Nam Vu-Duc |
| collection | DOAJ |
| description | An ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hypersil Gold PFP column utilizing a gradient of the mobile phase consisting of 5 mM ammonium formate, 0.1% formic acid in deionized water, and methanol. The target analytes were detected in the full-scan mode on Thermo Scientific Q-Exactive Focus Orbitrap MS for quantitation at the optimum operating conditions. These conditions included, but not limit to, the resolution of 70000 at the full width at half maximum in both positive and negative mode, mass range from 80 to 1000 m/z, and optimized parameters for the heated electrospray ionization source. The identification of the analytes in real samples was based on retention times, mass to charge ratios, mass accuracies, and MS/MS spectra at the confirmation mode with the inclusion list of target analytes. The mass accuracies of target analytes were from −4.14 ppm (dinotefuran) to 1.42 ppm (cinosulfuron) in the neat solvent and from −3.91 ppm (spinosad D) to 1.29 ppm (cinosulfuron) in the matrix-matched solution. Target analytes in the vegetable-based matrix were extracted by the QuEChERS method. Some critical parameters of the analytical method such as linearity, repeatability, limit of detection, and limit of quantitation have been evaluated and implemented. Excellent LOD and LOQ of the developed method were achieved at the range of 0.04–0.85 and 0.13–2.9 μg·kg−1, respectively. Intraday and interday repeatability of the analytical signal (peak area, n=6) of the developed method were below 3% and 10%, correspondingly. The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. Finally, the in-house validated method was applied to pesticides screening in vegetables samples in local villages in Hanoi, Vietnam. The concentrations of all target analytes were below limit of quantitation and lower than US-FDA or EU maximum residue levels. |
| format | Article |
| id | doaj-art-4e26fefd5e3845d6a20f1ad14bbd0ed5 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-4e26fefd5e3845d6a20f1ad14bbd0ed52025-02-03T01:09:28ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/34896343489634Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS)Nam Vu-Duc0Trung Nguyen-Quang1Thuy Le-Minh2Xuyen Nguyen-Thi3Tri Manh Tran4Hai Anh Vu5Lan-Anh Nguyen6Tien Doan-Duy7Bui Van Hoi8Cam-Tu Vu9Dung Le-Van10Lan-Anh Phung-Thi11Hong-An Vu-Thi12Dinh Binh Chu13Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamCenter for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamCenter for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamCenter for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hanoi 100000, VietnamInstitute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, VietnamDepartment of Water-Environment-Oceanography, University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamDepartment of Water-Environment-Oceanography, University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, VietnamDepartment of Chemistry, Vietnam Military Medical University, 160 Phung Hung, Hadong, Hanoi 100000, VietnamSchool of Environmental Science and Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hanoi 100000, VietnamDepartment of Analytical Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet, Hanoi 100000, VietnamDepartment of Analytical Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet, Hanoi 100000, VietnamAn ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hypersil Gold PFP column utilizing a gradient of the mobile phase consisting of 5 mM ammonium formate, 0.1% formic acid in deionized water, and methanol. The target analytes were detected in the full-scan mode on Thermo Scientific Q-Exactive Focus Orbitrap MS for quantitation at the optimum operating conditions. These conditions included, but not limit to, the resolution of 70000 at the full width at half maximum in both positive and negative mode, mass range from 80 to 1000 m/z, and optimized parameters for the heated electrospray ionization source. The identification of the analytes in real samples was based on retention times, mass to charge ratios, mass accuracies, and MS/MS spectra at the confirmation mode with the inclusion list of target analytes. The mass accuracies of target analytes were from −4.14 ppm (dinotefuran) to 1.42 ppm (cinosulfuron) in the neat solvent and from −3.91 ppm (spinosad D) to 1.29 ppm (cinosulfuron) in the matrix-matched solution. Target analytes in the vegetable-based matrix were extracted by the QuEChERS method. Some critical parameters of the analytical method such as linearity, repeatability, limit of detection, and limit of quantitation have been evaluated and implemented. Excellent LOD and LOQ of the developed method were achieved at the range of 0.04–0.85 and 0.13–2.9 μg·kg−1, respectively. Intraday and interday repeatability of the analytical signal (peak area, n=6) of the developed method were below 3% and 10%, correspondingly. The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. Finally, the in-house validated method was applied to pesticides screening in vegetables samples in local villages in Hanoi, Vietnam. The concentrations of all target analytes were below limit of quantitation and lower than US-FDA or EU maximum residue levels.http://dx.doi.org/10.1155/2019/3489634 |
| spellingShingle | Nam Vu-Duc Trung Nguyen-Quang Thuy Le-Minh Xuyen Nguyen-Thi Tri Manh Tran Hai Anh Vu Lan-Anh Nguyen Tien Doan-Duy Bui Van Hoi Cam-Tu Vu Dung Le-Van Lan-Anh Phung-Thi Hong-An Vu-Thi Dinh Binh Chu Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) Journal of Analytical Methods in Chemistry |
| title | Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) |
| title_full | Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) |
| title_fullStr | Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) |
| title_full_unstemmed | Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) |
| title_short | Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) |
| title_sort | multiresidue pesticides analysis of vegetables in vietnam by ultrahigh performance liquid chromatography in combination with high resolution mass spectrometry uplc orbitrap ms |
| url | http://dx.doi.org/10.1155/2019/3489634 |
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