A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response
Abstract The zinc finger protein 32 (ZNF32) has been associated with high expression in various cancers, underscoring its significant function in both cancer biology and immune response. To further elucidate the biological role of ZNF32 and identify potential immunotherapy targets in cancer, we cond...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-01803-0 |
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| author | Minghan Li Chang Su Qianru Wang Yuetong Chen Di Jiang Weijia Wang Shunjin Chen Xiangping Li Ming Fu Juan Lu |
| author_facet | Minghan Li Chang Su Qianru Wang Yuetong Chen Di Jiang Weijia Wang Shunjin Chen Xiangping Li Ming Fu Juan Lu |
| author_sort | Minghan Li |
| collection | DOAJ |
| description | Abstract The zinc finger protein 32 (ZNF32) has been associated with high expression in various cancers, underscoring its significant function in both cancer biology and immune response. To further elucidate the biological role of ZNF32 and identify potential immunotherapy targets in cancer, we conducted an in-depth analysis of ZNF32. We comprehensively investigated the expression of ZNF32 across tumors using diverse databases, including TCGA, CCLE, TIMER2.0, KM-Plotter, cBioPortal, ImmuCellAI. We investigated correlations between ZNF32 expression and various factors such as prognosis, immune infiltration, immunotherapy, DNA methylation, and biological functions. Furthermore, we performed in vitro research to validate the significance of ZNF32 in head and neck cancer (HNSC). Our study revealed that ZNF32 was high in various types of cancer, including ACC, BRCA, and others, indicating its important potential as a prognostic biomarker. Significant changes in CNA and DNA methylation were associated with high ZNF32 expression. ZNF32 was notably linked to various immune characteristics, including immune cell infiltration, MSI, TMB and immune checkpoint gene expression, indicating its potential in informing immunotherapy approaches. Interestingly, in FaDu and CAL27 cell lines, the group with elevated ZNF32 expression exhibited increased levels of immune checkpoint markers, such as CTLA-4 and PD-L1. Overexpression of ZNF32 significantly enhanced proliferation and migration in FaDu and CAL27 cell lines, as demonstrated through CCK-8 assays, colony formation, flow cytometry, Transwell migration, and Boyden invasion assays. Our in vitro experiments confirmed that ZNF32 promotes malignant behavior by driving HNSC cell proliferation and migration. These results imply that ZNF32 might be a promising target for tumor prognosis and immunotherapy. Our results highlight the important role of ZNF32 in tumorigenesis and provide novel perspectives for potential cancer treatment strategies. |
| format | Article |
| id | doaj-art-4e25de5ca8a74accb13483d4be47e693 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-4e25de5ca8a74accb13483d4be47e6932025-02-02T12:30:38ZengSpringerDiscover Oncology2730-60112025-01-0116111810.1007/s12672-025-01803-0A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy responseMinghan Li0Chang Su1Qianru Wang2Yuetong Chen3Di Jiang4Weijia Wang5Shunjin Chen6Xiangping Li7Ming Fu8Juan Lu9Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityThe First School of Clinical Medicine, Southern Medical UniversityDepartment of Otolaryngology, The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital)Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Otolaryngology, The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital)Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Otolaryngology, The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital)Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical UniversityAbstract The zinc finger protein 32 (ZNF32) has been associated with high expression in various cancers, underscoring its significant function in both cancer biology and immune response. To further elucidate the biological role of ZNF32 and identify potential immunotherapy targets in cancer, we conducted an in-depth analysis of ZNF32. We comprehensively investigated the expression of ZNF32 across tumors using diverse databases, including TCGA, CCLE, TIMER2.0, KM-Plotter, cBioPortal, ImmuCellAI. We investigated correlations between ZNF32 expression and various factors such as prognosis, immune infiltration, immunotherapy, DNA methylation, and biological functions. Furthermore, we performed in vitro research to validate the significance of ZNF32 in head and neck cancer (HNSC). Our study revealed that ZNF32 was high in various types of cancer, including ACC, BRCA, and others, indicating its important potential as a prognostic biomarker. Significant changes in CNA and DNA methylation were associated with high ZNF32 expression. ZNF32 was notably linked to various immune characteristics, including immune cell infiltration, MSI, TMB and immune checkpoint gene expression, indicating its potential in informing immunotherapy approaches. Interestingly, in FaDu and CAL27 cell lines, the group with elevated ZNF32 expression exhibited increased levels of immune checkpoint markers, such as CTLA-4 and PD-L1. Overexpression of ZNF32 significantly enhanced proliferation and migration in FaDu and CAL27 cell lines, as demonstrated through CCK-8 assays, colony formation, flow cytometry, Transwell migration, and Boyden invasion assays. Our in vitro experiments confirmed that ZNF32 promotes malignant behavior by driving HNSC cell proliferation and migration. These results imply that ZNF32 might be a promising target for tumor prognosis and immunotherapy. Our results highlight the important role of ZNF32 in tumorigenesis and provide novel perspectives for potential cancer treatment strategies.https://doi.org/10.1007/s12672-025-01803-0ZNF32Pan-cancerPrognosisBiomarkerImmunotherapy |
| spellingShingle | Minghan Li Chang Su Qianru Wang Yuetong Chen Di Jiang Weijia Wang Shunjin Chen Xiangping Li Ming Fu Juan Lu A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response Discover Oncology ZNF32 Pan-cancer Prognosis Biomarker Immunotherapy |
| title | A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response |
| title_full | A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response |
| title_fullStr | A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response |
| title_full_unstemmed | A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response |
| title_short | A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response |
| title_sort | pan cancer analysis predictive role of znf32 in cancer prognosis and immunotherapy response |
| topic | ZNF32 Pan-cancer Prognosis Biomarker Immunotherapy |
| url | https://doi.org/10.1007/s12672-025-01803-0 |
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