Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development
The hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism. The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system. The avian hypothalamus due to the structural complexity is not well des...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/6049469 |
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| author | Katarzyna Piórkowska Kacper Żukowski Katarzyna Połtowicz Joanna Nowak Dorota Wojtysiak Natalia Derebecka Joanna Wesoły Katarzyna Ropka-Molik |
| author_facet | Katarzyna Piórkowska Kacper Żukowski Katarzyna Połtowicz Joanna Nowak Dorota Wojtysiak Natalia Derebecka Joanna Wesoły Katarzyna Ropka-Molik |
| author_sort | Katarzyna Piórkowska |
| collection | DOAJ |
| description | The hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism. The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system. The avian hypothalamus due to the structural complexity is not well described and has a slightly different function than the mammalian hypothalamus that is the subject of numerous studies. The present study evaluated activities of hypothalamic genes in fast-growing chickens during development (at the 1st day and 3rd and 6th weeks after hatching). The hypothalamic transcriptomes for 3- and 6-week-old cockerels were analysed using an RNA sequencing method in next-generation sequencing (NGS) technology. The differentially expressed gene analysis was conducted using DESeq2 software. In younger 22-day-old cockerels, 389 genes showed higher expression (fold change > 1.5) than that in 45-day-old birds. These genes played a role in several biological processes because they encoded proteins involved in integrin signalling, regulation of hormone levels, camera-type eye development, and blood vessel development. Moreover, surprisingly in the hypothalamus of 3-week-old cockerels, transcripts were identified for proteins involved in both anorexigenic (POMC, NMU) and orexigenic (PMCH, ALDH1A1, LPL, and GHRH) pathways. The RNA-seq results were confirmed by qPCR methods. In summary, the intensive growth of 3-week-old chickens was reflected in hypothalamic activities because the genes associated with the somatotropin axis and regulation of satiety centre showed increased expression. |
| format | Article |
| id | doaj-art-4e257548538240e2a4abfe2f0587c2cd |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4e257548538240e2a4abfe2f0587c2cd2025-08-20T02:38:46ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/60494696049469Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and DevelopmentKatarzyna Piórkowska0Kacper Żukowski1Katarzyna Połtowicz2Joanna Nowak3Dorota Wojtysiak4Natalia Derebecka5Joanna Wesoły6Katarzyna Ropka-Molik7Department of Molecular Biology, National Research Institute of Animal Production, Balice 32-083, PolandDepartment of Cattle Breeding, National Research Institute of Animal Production, Balice 32-083, PolandDepartment of Poultry Breeding, National Research Institute of Animal Production, Balice 32-083, PolandDepartment of Poultry Breeding, National Research Institute of Animal Production, Balice 32-083, PolandInstitute of Veterinary Sciences, Department of Animal Anatomy, Agricultural University of Cracow, Kraków 30-059, PolandLaboratory of High Throughput Technologies, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznań, PolandLaboratory of High Throughput Technologies, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznań, PolandDepartment of Molecular Biology, National Research Institute of Animal Production, Balice 32-083, PolandThe hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism. The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system. The avian hypothalamus due to the structural complexity is not well described and has a slightly different function than the mammalian hypothalamus that is the subject of numerous studies. The present study evaluated activities of hypothalamic genes in fast-growing chickens during development (at the 1st day and 3rd and 6th weeks after hatching). The hypothalamic transcriptomes for 3- and 6-week-old cockerels were analysed using an RNA sequencing method in next-generation sequencing (NGS) technology. The differentially expressed gene analysis was conducted using DESeq2 software. In younger 22-day-old cockerels, 389 genes showed higher expression (fold change > 1.5) than that in 45-day-old birds. These genes played a role in several biological processes because they encoded proteins involved in integrin signalling, regulation of hormone levels, camera-type eye development, and blood vessel development. Moreover, surprisingly in the hypothalamus of 3-week-old cockerels, transcripts were identified for proteins involved in both anorexigenic (POMC, NMU) and orexigenic (PMCH, ALDH1A1, LPL, and GHRH) pathways. The RNA-seq results were confirmed by qPCR methods. In summary, the intensive growth of 3-week-old chickens was reflected in hypothalamic activities because the genes associated with the somatotropin axis and regulation of satiety centre showed increased expression.http://dx.doi.org/10.1155/2018/6049469 |
| spellingShingle | Katarzyna Piórkowska Kacper Żukowski Katarzyna Połtowicz Joanna Nowak Dorota Wojtysiak Natalia Derebecka Joanna Wesoły Katarzyna Ropka-Molik Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development International Journal of Genomics |
| title | Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development |
| title_full | Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development |
| title_fullStr | Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development |
| title_full_unstemmed | Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development |
| title_short | Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development |
| title_sort | transcriptomic changes in broiler chicken hypothalamus during growth and development |
| url | http://dx.doi.org/10.1155/2018/6049469 |
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