Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.

Otitis media (OM), inflammation of the middle ear, remains the most common cause of hearing impairment in children. It is also the most common cause of surgery in children in the developed world. There is evidence from studies of the human population and mouse models that there is a significant gene...

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Main Authors: Nicholas Parkinson, Rachel E Hardisty-Hughes, Hilda Tateossian, Hsun-Tien Tsai, Debra Brooker, Sue Morse, Zuzanna Lalane, Francesca MacKenzie, Martin Fray, Pete Glenister, Anne-Marie Woodward, Sian Polley, Ivana Barbaric, Neil Dear, Tertius A Hough, A Jackie Hunter, Michael T Cheeseman, Steve D M Brown
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2006-10-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.0020149&type=printable
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author Nicholas Parkinson
Rachel E Hardisty-Hughes
Hilda Tateossian
Hsun-Tien Tsai
Debra Brooker
Sue Morse
Zuzanna Lalane
Francesca MacKenzie
Martin Fray
Pete Glenister
Anne-Marie Woodward
Sian Polley
Ivana Barbaric
Neil Dear
Tertius A Hough
A Jackie Hunter
Michael T Cheeseman
Steve D M Brown
author_facet Nicholas Parkinson
Rachel E Hardisty-Hughes
Hilda Tateossian
Hsun-Tien Tsai
Debra Brooker
Sue Morse
Zuzanna Lalane
Francesca MacKenzie
Martin Fray
Pete Glenister
Anne-Marie Woodward
Sian Polley
Ivana Barbaric
Neil Dear
Tertius A Hough
A Jackie Hunter
Michael T Cheeseman
Steve D M Brown
author_sort Nicholas Parkinson
collection DOAJ
description Otitis media (OM), inflammation of the middle ear, remains the most common cause of hearing impairment in children. It is also the most common cause of surgery in children in the developed world. There is evidence from studies of the human population and mouse models that there is a significant genetic component predisposing to OM, yet nothing is known about the underlying genetic pathways involved in humans. We identified an N-ethyl-N-nitrosourea-induced dominant mouse mutant Junbo with hearing loss due to chronic suppurative OM and otorrhea. This develops from acute OM that arises spontaneously in the postnatal period, with the age of onset and early severity dependent on the microbiological status of the mice and their air quality. We have identified the causal mutation, a missense change in the C-terminal zinc finger region of the transcription factor Evi1. This protein is expressed in middle ear basal epithelial cells, fibroblasts, and neutrophil leukocytes at postnatal day 13 and 21 when inflammatory changes are underway. The identification and characterization of the Junbo mutant elaborates a novel role for Evi1 in mammalian disease and implicates a new pathway in genetic predisposition to OM.
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spelling doaj-art-4e04b3f64b7f43c8b014fa9eef6c1a5c2025-08-20T02:17:24ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042006-10-01210e14910.1371/journal.pgen.0020149Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.Nicholas ParkinsonRachel E Hardisty-HughesHilda TateossianHsun-Tien TsaiDebra BrookerSue MorseZuzanna LalaneFrancesca MacKenzieMartin FrayPete GlenisterAnne-Marie WoodwardSian PolleyIvana BarbaricNeil DearTertius A HoughA Jackie HunterMichael T CheesemanSteve D M BrownOtitis media (OM), inflammation of the middle ear, remains the most common cause of hearing impairment in children. It is also the most common cause of surgery in children in the developed world. There is evidence from studies of the human population and mouse models that there is a significant genetic component predisposing to OM, yet nothing is known about the underlying genetic pathways involved in humans. We identified an N-ethyl-N-nitrosourea-induced dominant mouse mutant Junbo with hearing loss due to chronic suppurative OM and otorrhea. This develops from acute OM that arises spontaneously in the postnatal period, with the age of onset and early severity dependent on the microbiological status of the mice and their air quality. We have identified the causal mutation, a missense change in the C-terminal zinc finger region of the transcription factor Evi1. This protein is expressed in middle ear basal epithelial cells, fibroblasts, and neutrophil leukocytes at postnatal day 13 and 21 when inflammatory changes are underway. The identification and characterization of the Junbo mutant elaborates a novel role for Evi1 in mammalian disease and implicates a new pathway in genetic predisposition to OM.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.0020149&type=printable
spellingShingle Nicholas Parkinson
Rachel E Hardisty-Hughes
Hilda Tateossian
Hsun-Tien Tsai
Debra Brooker
Sue Morse
Zuzanna Lalane
Francesca MacKenzie
Martin Fray
Pete Glenister
Anne-Marie Woodward
Sian Polley
Ivana Barbaric
Neil Dear
Tertius A Hough
A Jackie Hunter
Michael T Cheeseman
Steve D M Brown
Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
PLoS Genetics
title Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
title_full Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
title_fullStr Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
title_full_unstemmed Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
title_short Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media.
title_sort mutation at the evi1 locus in junbo mice causes susceptibility to otitis media
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.0020149&type=printable
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