Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii
Abstract The development of newfangled bioprocess strategies for the capture of C1-gases (CO and CO2) and their bioconversion into valuable products is currently one of the main focuses of research in order to achieve a more resilient world. This work analyses the viability of the co-culture C. carb...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-05-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-025-00881-w |
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| author | Marina Fernández-Delgado Pedro Enrique Plaza M. Teresa García-Cubero Susana Lucas Mónica Coca Juan Carlos López-Linares |
| author_facet | Marina Fernández-Delgado Pedro Enrique Plaza M. Teresa García-Cubero Susana Lucas Mónica Coca Juan Carlos López-Linares |
| author_sort | Marina Fernández-Delgado |
| collection | DOAJ |
| description | Abstract The development of newfangled bioprocess strategies for the capture of C1-gases (CO and CO2) and their bioconversion into valuable products is currently one of the main focuses of research in order to achieve a more resilient world. This work analyses the viability of the co-culture C. carboxidivorans and C. beijerinkii to produce bioproducts (bioalcohols and organic acids) in mixotrophic conditions. In this way, the bioconversion of C1 gases (CO and CO2), in the presence of Fe0, using mixotrophic co-culture fermentation by C. carboxidivorans and C. beijerinkii, was evaluated, analyzing the influence of the ratio between both microorganisms, the pH, and the presence of Fe0. As a result, up to 7 g/L of butanol were achieved at pH 7, 12.5 g/L Fe0, and using a 1:1 ratio of C. carboxidivorans: C. beijerinkii, also improving the production of ethanol, acetic acid, and butyric acid as compared to individual culture fermentations. Finally, the operation in a bioreactor, comparing discontinuous and continuous gas feeding operation modes, was also studied, with better C1-gases utilization and overall fermentation efficiency (7 vs 4.6 g/L butanol) in continuous gas operation mode. |
| format | Article |
| id | doaj-art-4e040b5070a64458a4d97faec549bc69 |
| institution | DOAJ |
| issn | 2197-4365 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj-art-4e040b5070a64458a4d97faec549bc692025-08-20T03:16:33ZengSpringerOpenBioresources and Bioprocessing2197-43652025-05-0112111510.1186/s40643-025-00881-wBioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkiiMarina Fernández-Delgado0Pedro Enrique Plaza1M. Teresa García-Cubero2Susana Lucas3Mónica Coca4Juan Carlos López-Linares5Department of Chemical Engineering and Environmental Technology, University of ValladolidDepartment of Chemical Engineering and Environmental Technology, University of ValladolidDepartment of Chemical Engineering and Environmental Technology, University of ValladolidDepartment of Chemical Engineering and Environmental Technology, University of ValladolidDepartment of Chemical Engineering and Environmental Technology, University of ValladolidDepartment of Chemical Engineering and Environmental Technology, University of ValladolidAbstract The development of newfangled bioprocess strategies for the capture of C1-gases (CO and CO2) and their bioconversion into valuable products is currently one of the main focuses of research in order to achieve a more resilient world. This work analyses the viability of the co-culture C. carboxidivorans and C. beijerinkii to produce bioproducts (bioalcohols and organic acids) in mixotrophic conditions. In this way, the bioconversion of C1 gases (CO and CO2), in the presence of Fe0, using mixotrophic co-culture fermentation by C. carboxidivorans and C. beijerinkii, was evaluated, analyzing the influence of the ratio between both microorganisms, the pH, and the presence of Fe0. As a result, up to 7 g/L of butanol were achieved at pH 7, 12.5 g/L Fe0, and using a 1:1 ratio of C. carboxidivorans: C. beijerinkii, also improving the production of ethanol, acetic acid, and butyric acid as compared to individual culture fermentations. Finally, the operation in a bioreactor, comparing discontinuous and continuous gas feeding operation modes, was also studied, with better C1-gases utilization and overall fermentation efficiency (7 vs 4.6 g/L butanol) in continuous gas operation mode.https://doi.org/10.1186/s40643-025-00881-wC1-gases conversionMixotrophic fermentationFe0Co-cultureClostridia spp. |
| spellingShingle | Marina Fernández-Delgado Pedro Enrique Plaza M. Teresa García-Cubero Susana Lucas Mónica Coca Juan Carlos López-Linares Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii Bioresources and Bioprocessing C1-gases conversion Mixotrophic fermentation Fe0 Co-culture Clostridia spp. |
| title | Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii |
| title_full | Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii |
| title_fullStr | Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii |
| title_full_unstemmed | Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii |
| title_short | Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii |
| title_sort | bioconversion of c1 gases by mixotrophic co cultures fermentation with c carboxidivorans and c beijerinkii |
| topic | C1-gases conversion Mixotrophic fermentation Fe0 Co-culture Clostridia spp. |
| url | https://doi.org/10.1186/s40643-025-00881-w |
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