Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis
G. pentaphyllum (Gynostemma pentaphyllum), a creeping herbaceous perennial with many important medicinal properties, is widely distributed in Asia. Gypenosides (triterpenoid saponins), the main effective components of G. pentaphyllum, are well studied. FPS (farnesyl pyrophosphate synthase), SS (squa...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2016/7840914 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545985587314688 |
|---|---|
| author | Qicong Chen Chengtong Ma Jieying Qian Xiuwan Lan Naixia Chao Jian Sun Yaosheng Wu |
| author_facet | Qicong Chen Chengtong Ma Jieying Qian Xiuwan Lan Naixia Chao Jian Sun Yaosheng Wu |
| author_sort | Qicong Chen |
| collection | DOAJ |
| description | G. pentaphyllum (Gynostemma pentaphyllum), a creeping herbaceous perennial with many important medicinal properties, is widely distributed in Asia. Gypenosides (triterpenoid saponins), the main effective components of G. pentaphyllum, are well studied. FPS (farnesyl pyrophosphate synthase), SS (squalene synthase), and SE (squalene epoxidase) are the main enzymes involved in the synthesis of triterpenoid saponins. Considering the important medicinal functions of G. pentaphyllum, it is necessary to investigate the transcriptomic information of G. pentaphyllum to facilitate future studies of transcriptional regulation. After sequencing G. pentaphyllum, we obtained 50,654,708 unigenes. Next, we used RPKM (reads per kilobases per million reads) to calculate expression of the unigenes and we performed comparison of our data to that contained in five common databases to annotate different aspects of the unigenes. Finally, we noticed that FPS, SS, and SE showed differential expression of enzymes in DESeq. Leaves showed the highest expression of FPS, SS, and SE relative to the other two tissues. Our research provides transcriptomic information of G. pentaphyllum in its natural environment and we found consistency in unigene expression, enzymes expression (FPS, SS, and SE), and the distribution of gypenosides content in G. pentaphyllum. Our results will enable future related studies of G. pentaphyllum. |
| format | Article |
| id | doaj-art-4df908c5c7044b48b5fff0aa0f342af3 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4df908c5c7044b48b5fff0aa0f342af32025-02-03T07:24:04ZengWileyInternational Journal of Genomics2314-436X2314-43782016-01-01201610.1155/2016/78409147840914Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid BiosynthesisQicong Chen0Chengtong Ma1Jieying Qian2Xiuwan Lan3Naixia Chao4Jian Sun5Yaosheng Wu6Key Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaKey Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, ChinaG. pentaphyllum (Gynostemma pentaphyllum), a creeping herbaceous perennial with many important medicinal properties, is widely distributed in Asia. Gypenosides (triterpenoid saponins), the main effective components of G. pentaphyllum, are well studied. FPS (farnesyl pyrophosphate synthase), SS (squalene synthase), and SE (squalene epoxidase) are the main enzymes involved in the synthesis of triterpenoid saponins. Considering the important medicinal functions of G. pentaphyllum, it is necessary to investigate the transcriptomic information of G. pentaphyllum to facilitate future studies of transcriptional regulation. After sequencing G. pentaphyllum, we obtained 50,654,708 unigenes. Next, we used RPKM (reads per kilobases per million reads) to calculate expression of the unigenes and we performed comparison of our data to that contained in five common databases to annotate different aspects of the unigenes. Finally, we noticed that FPS, SS, and SE showed differential expression of enzymes in DESeq. Leaves showed the highest expression of FPS, SS, and SE relative to the other two tissues. Our research provides transcriptomic information of G. pentaphyllum in its natural environment and we found consistency in unigene expression, enzymes expression (FPS, SS, and SE), and the distribution of gypenosides content in G. pentaphyllum. Our results will enable future related studies of G. pentaphyllum.http://dx.doi.org/10.1155/2016/7840914 |
| spellingShingle | Qicong Chen Chengtong Ma Jieying Qian Xiuwan Lan Naixia Chao Jian Sun Yaosheng Wu Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis International Journal of Genomics |
| title | Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis |
| title_full | Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis |
| title_fullStr | Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis |
| title_full_unstemmed | Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis |
| title_short | Transcriptome Sequencing of Gynostemma pentaphyllum to Identify Genes and Enzymes Involved in Triterpenoid Biosynthesis |
| title_sort | transcriptome sequencing of gynostemma pentaphyllum to identify genes and enzymes involved in triterpenoid biosynthesis |
| url | http://dx.doi.org/10.1155/2016/7840914 |
| work_keys_str_mv | AT qicongchen transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT chengtongma transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT jieyingqian transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT xiuwanlan transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT naixiachao transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT jiansun transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis AT yaoshengwu transcriptomesequencingofgynostemmapentaphyllumtoidentifygenesandenzymesinvolvedintriterpenoidbiosynthesis |