Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA
One of the measures used to control and prevent African swine fever spread in the Russian Federation involves testing pig and boar sera using inter alia serological tools based on enzyme-linked immunosorbent assay (ELISA) for anti-viral antibody detection. However, there is no unified regulatory doc...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2021-08-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/574 |
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| author | A. R. Shotin I. Yu. Zhukov A. S. Pershin Ali Mazloum I. V. Shevchenko A. S. Igolkin O. A. Manuylova K. N. Gruzdev |
| author_facet | A. R. Shotin I. Yu. Zhukov A. S. Pershin Ali Mazloum I. V. Shevchenko A. S. Igolkin O. A. Manuylova K. N. Gruzdev |
| author_sort | A. R. Shotin |
| collection | DOAJ |
| description | One of the measures used to control and prevent African swine fever spread in the Russian Federation involves testing pig and boar sera using inter alia serological tools based on enzyme-linked immunosorbent assay (ELISA) for anti-viral antibody detection. However, there is no unified regulatory document specifying storage conditions for sera used in the ELISA for anti-ASFV antibody detection. There are also lack of published data on the maximum admissible shelf life of the pig sera, and the effect of storage conditions on the serological status of the pig sera as for ASF is understudied. The paper demonstrates results of the experiment aimed at the determination of the effect of storage temperatures and shelf life on the serological status of ASFV seropositive and seronegative pig sera when tested by INgezim PPA Compac (Ingenasa, Spain) ELISA as well as on the possibility of false results. During the experiment and analysis of its results, the new data were obtained, and they indicated from none to non-significant effect of the simulated storage conditions on the serological status of sera used for ASFV detection, while hemolyzed sera demonstrated more significant changes proportional to hemolysis degree and storage duration. Although the results of detection of antibodies against the agents of some diseases cannot be used in case of other pathogens, this study has a substantial applied significance as it allows to specify the dependence of the valid results of ASF serodiagnosis on the storage conditions of the samples. |
| format | Article |
| id | doaj-art-4dea5b7fb6de4d50aee2d3b4146a9bb6 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-4dea5b7fb6de4d50aee2d3b4146a9bb62025-02-06T09:52:08ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-08-0110321622310.29326/2304-196X-2021-3-38-216-223521Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISAA. R. Shotin0I. Yu. Zhukov1A. S. Pershin2Ali Mazloum3I. V. Shevchenko4A. S. Igolkin5O. A. Manuylova6K. N. Gruzdev7FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”One of the measures used to control and prevent African swine fever spread in the Russian Federation involves testing pig and boar sera using inter alia serological tools based on enzyme-linked immunosorbent assay (ELISA) for anti-viral antibody detection. However, there is no unified regulatory document specifying storage conditions for sera used in the ELISA for anti-ASFV antibody detection. There are also lack of published data on the maximum admissible shelf life of the pig sera, and the effect of storage conditions on the serological status of the pig sera as for ASF is understudied. The paper demonstrates results of the experiment aimed at the determination of the effect of storage temperatures and shelf life on the serological status of ASFV seropositive and seronegative pig sera when tested by INgezim PPA Compac (Ingenasa, Spain) ELISA as well as on the possibility of false results. During the experiment and analysis of its results, the new data were obtained, and they indicated from none to non-significant effect of the simulated storage conditions on the serological status of sera used for ASFV detection, while hemolyzed sera demonstrated more significant changes proportional to hemolysis degree and storage duration. Although the results of detection of antibodies against the agents of some diseases cannot be used in case of other pathogens, this study has a substantial applied significance as it allows to specify the dependence of the valid results of ASF serodiagnosis on the storage conditions of the samples.https://veterinary.arriah.ru/jour/article/view/574african swine feverenzyme-linked immunosorbent assayserumstorage conditions |
| spellingShingle | A. R. Shotin I. Yu. Zhukov A. S. Pershin Ali Mazloum I. V. Shevchenko A. S. Igolkin O. A. Manuylova K. N. Gruzdev Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA Ветеринария сегодня african swine fever enzyme-linked immunosorbent assay serum storage conditions |
| title | Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA |
| title_full | Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA |
| title_fullStr | Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA |
| title_full_unstemmed | Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA |
| title_short | Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA |
| title_sort | effect of pig serum storage conditions on detection of anti asfv antibodies by elisa |
| topic | african swine fever enzyme-linked immunosorbent assay serum storage conditions |
| url | https://veterinary.arriah.ru/jour/article/view/574 |
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