Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells
This study evaluates the potential of biorefinery and dairy wastewater as substrates for electricity generation in double chamber Microbial Fuel Cells (DCMFC), focusing on their microbial taxonomy and electrochemical viability. Taxonomic analysis using 16S/18S rDNA-targeted DGGE and high-throughput...
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MDPI AG
2025-01-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/12/1/88 |
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| author | Khaya Pearlman Shabangu Manimagalay Chetty Babatunde Femi Bakare |
| author_facet | Khaya Pearlman Shabangu Manimagalay Chetty Babatunde Femi Bakare |
| author_sort | Khaya Pearlman Shabangu |
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| description | This study evaluates the potential of biorefinery and dairy wastewater as substrates for electricity generation in double chamber Microbial Fuel Cells (DCMFC), focusing on their microbial taxonomy and electrochemical viability. Taxonomic analysis using 16S/18S rDNA-targeted DGGE and high-throughput sequencing identified Proteobacteria as dominant in biorefinery biomass, followed by Firmicutes and Bacteriodota. In dairy biomass, Lactobacillus (77.36%) and Clostridium (15.70%) were most prevalent. Biorefinery wastewater exhibited the highest bioelectrochemical viability due to its superior electrical conductivity and salinity, achieving a voltage yield of 65 mV, compared to 75.2 mV from mixed substrates and 1.7 mV from dairy wastewater. Elevated phosphate levels in dairy wastewater inhibited bioelectrochemical processes. This study recommends Biorefinery wastewater as the most suitable purely organic substrate for efficient bioelectricity generation and scaling up of MFCs, emphasising the importance of substrate selection for optimal energy output for practical and commercial viability. |
| format | Article |
| id | doaj-art-4dd35248965f4bea810f7e393adbc909 |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj-art-4dd35248965f4bea810f7e393adbc9092025-01-24T13:23:14ZengMDPI AGBioengineering2306-53542025-01-011218810.3390/bioengineering12010088Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel CellsKhaya Pearlman Shabangu0Manimagalay Chetty1Babatunde Femi Bakare2Green Engineering & Sustainability Research Group, Department of Chemical Engineering, Faculty of Engineering and the Built Environment, Durban University of Technology, Steve Biko Campus, S3 L3, P.O. Box 1334, Durban 4000, South AfricaGreen Engineering & Sustainability Research Group, Department of Chemical Engineering, Faculty of Engineering and the Built Environment, Durban University of Technology, Steve Biko Campus, S3 L3, P.O. Box 1334, Durban 4000, South AfricaEnvironmental Pollution and Remediation Research Group, Department of Chemical Engineering, Mangosuthu University of Technology, P.O. Box 12363, Jacobs 4062, South AfricaThis study evaluates the potential of biorefinery and dairy wastewater as substrates for electricity generation in double chamber Microbial Fuel Cells (DCMFC), focusing on their microbial taxonomy and electrochemical viability. Taxonomic analysis using 16S/18S rDNA-targeted DGGE and high-throughput sequencing identified Proteobacteria as dominant in biorefinery biomass, followed by Firmicutes and Bacteriodota. In dairy biomass, Lactobacillus (77.36%) and Clostridium (15.70%) were most prevalent. Biorefinery wastewater exhibited the highest bioelectrochemical viability due to its superior electrical conductivity and salinity, achieving a voltage yield of 65 mV, compared to 75.2 mV from mixed substrates and 1.7 mV from dairy wastewater. Elevated phosphate levels in dairy wastewater inhibited bioelectrochemical processes. This study recommends Biorefinery wastewater as the most suitable purely organic substrate for efficient bioelectricity generation and scaling up of MFCs, emphasising the importance of substrate selection for optimal energy output for practical and commercial viability.https://www.mdpi.com/2306-5354/12/1/88Bacteriodotaelectrical conductivityFirmicutesMicrobial Fuel CellProteobacteriasalinity |
| spellingShingle | Khaya Pearlman Shabangu Manimagalay Chetty Babatunde Femi Bakare Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells Bioengineering Bacteriodota electrical conductivity Firmicutes Microbial Fuel Cell Proteobacteria salinity |
| title | Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells |
| title_full | Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells |
| title_fullStr | Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells |
| title_full_unstemmed | Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells |
| title_short | Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells |
| title_sort | metagenomic insights into pollutants in biorefinery and dairy wastewater rdna dominance and electricity generation in double chamber microbial fuel cells |
| topic | Bacteriodota electrical conductivity Firmicutes Microbial Fuel Cell Proteobacteria salinity |
| url | https://www.mdpi.com/2306-5354/12/1/88 |
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