Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study
Background: Vitamin E, a fat-soluble vitamin, protects cell membranes by scavenging free radicals and reducing lipid peroxidation. It plays a crucial role in immune cell function, gene expression, and modulation of inflammatory mediators. Nanoparticles in drug delivery, particularly chitosan-based n...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Journal of Indian Academy of Oral Medicine and Radiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jiaomr.jiaomr_265_24 |
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| author | Turaga Amani Mouttoukichenin Surenthar |
| author_facet | Turaga Amani Mouttoukichenin Surenthar |
| author_sort | Turaga Amani |
| collection | DOAJ |
| description | Background:
Vitamin E, a fat-soluble vitamin, protects cell membranes by scavenging free radicals and reducing lipid peroxidation. It plays a crucial role in immune cell function, gene expression, and modulation of inflammatory mediators. Nanoparticles in drug delivery, particularly chitosan-based nanogels, enhance solubility and prolong biological activity.
Objective:
This study aims to evaluate the antioxidant and anti-inflammatory activity of a vitamin E-infused nanogel formulation in an in-vitro setting and compare its efficacy with standard antioxidant and anti-inflammatory agents.
Methods:
A gel was created by lyophilizing 5 g of powdered vitamin E, 0.5 g of chitosan, 0.2 mL of tween 80, and 10 mL of monosodium phosphate in 90 mL of distilled water, which was then heated to 55°C for 30 minutes while stirring. The formulation’s antioxidant and anti-inflammatory activities were assessed using standard assays, such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and albumin denaturation assays. The nanogel’s effectiveness was assessed by comparing its percentage inhibition values at different concentrations with those of standard antioxidants (ascorbic acid) and anti-inflammatory agents (diclofenac sodium).
Results:
The vitamin E-infused nanogel demonstrated antioxidant and anti-inflammatory properties comparable to standard drugs, with the highest activity observed at 100 µg/mL in both assays.
Conclusion:
The study demonstrates that the vitamin E-infused nanogel exhibits promising in-vitro antioxidant and anti-inflammatory activities, with inhibition percentages comparable to standard drugs. Further validation through statistical analysis and in-vivo studies is required to confirm its therapeutic potential in managing oral potentially malignant disorders. |
| format | Article |
| id | doaj-art-4dcb45ba9ef4491ebe9aa8c204345a1d |
| institution | OA Journals |
| issn | 0972-1363 0975-1572 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Academy of Oral Medicine and Radiology |
| spelling | doaj-art-4dcb45ba9ef4491ebe9aa8c204345a1d2025-08-20T02:11:34ZengWolters Kluwer Medknow PublicationsJournal of Indian Academy of Oral Medicine and Radiology0972-13630975-15722025-01-01371788210.4103/jiaomr.jiaomr_265_24Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro StudyTuraga AmaniMouttoukichenin SurentharBackground: Vitamin E, a fat-soluble vitamin, protects cell membranes by scavenging free radicals and reducing lipid peroxidation. It plays a crucial role in immune cell function, gene expression, and modulation of inflammatory mediators. Nanoparticles in drug delivery, particularly chitosan-based nanogels, enhance solubility and prolong biological activity. Objective: This study aims to evaluate the antioxidant and anti-inflammatory activity of a vitamin E-infused nanogel formulation in an in-vitro setting and compare its efficacy with standard antioxidant and anti-inflammatory agents. Methods: A gel was created by lyophilizing 5 g of powdered vitamin E, 0.5 g of chitosan, 0.2 mL of tween 80, and 10 mL of monosodium phosphate in 90 mL of distilled water, which was then heated to 55°C for 30 minutes while stirring. The formulation’s antioxidant and anti-inflammatory activities were assessed using standard assays, such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and albumin denaturation assays. The nanogel’s effectiveness was assessed by comparing its percentage inhibition values at different concentrations with those of standard antioxidants (ascorbic acid) and anti-inflammatory agents (diclofenac sodium). Results: The vitamin E-infused nanogel demonstrated antioxidant and anti-inflammatory properties comparable to standard drugs, with the highest activity observed at 100 µg/mL in both assays. Conclusion: The study demonstrates that the vitamin E-infused nanogel exhibits promising in-vitro antioxidant and anti-inflammatory activities, with inhibition percentages comparable to standard drugs. Further validation through statistical analysis and in-vivo studies is required to confirm its therapeutic potential in managing oral potentially malignant disorders.https://journals.lww.com/10.4103/jiaomr.jiaomr_265_24anti-inflammatory agentsantioxidantschitosannanoparticle drug delivery systemvitamin e |
| spellingShingle | Turaga Amani Mouttoukichenin Surenthar Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study Journal of Indian Academy of Oral Medicine and Radiology anti-inflammatory agents antioxidants chitosan nanoparticle drug delivery system vitamin e |
| title | Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study |
| title_full | Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study |
| title_fullStr | Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study |
| title_full_unstemmed | Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study |
| title_short | Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study |
| title_sort | anti inflammatory and antioxidant activity of vitamin e infused nanogel formulation an in vitro study |
| topic | anti-inflammatory agents antioxidants chitosan nanoparticle drug delivery system vitamin e |
| url | https://journals.lww.com/10.4103/jiaomr.jiaomr_265_24 |
| work_keys_str_mv | AT turagaamani antiinflammatoryandantioxidantactivityofvitamineinfusednanogelformulationaninvitrostudy AT mouttoukicheninsurenthar antiinflammatoryandantioxidantactivityofvitamineinfusednanogelformulationaninvitrostudy |