Anti-Inflammatory and Antioxidant Activity of Vitamin E-infused Nanogel Formulation: An In-Vitro Study
Background: Vitamin E, a fat-soluble vitamin, protects cell membranes by scavenging free radicals and reducing lipid peroxidation. It plays a crucial role in immune cell function, gene expression, and modulation of inflammatory mediators. Nanoparticles in drug delivery, particularly chitosan-based n...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Journal of Indian Academy of Oral Medicine and Radiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jiaomr.jiaomr_265_24 |
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| Summary: | Background:
Vitamin E, a fat-soluble vitamin, protects cell membranes by scavenging free radicals and reducing lipid peroxidation. It plays a crucial role in immune cell function, gene expression, and modulation of inflammatory mediators. Nanoparticles in drug delivery, particularly chitosan-based nanogels, enhance solubility and prolong biological activity.
Objective:
This study aims to evaluate the antioxidant and anti-inflammatory activity of a vitamin E-infused nanogel formulation in an in-vitro setting and compare its efficacy with standard antioxidant and anti-inflammatory agents.
Methods:
A gel was created by lyophilizing 5 g of powdered vitamin E, 0.5 g of chitosan, 0.2 mL of tween 80, and 10 mL of monosodium phosphate in 90 mL of distilled water, which was then heated to 55°C for 30 minutes while stirring. The formulation’s antioxidant and anti-inflammatory activities were assessed using standard assays, such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and albumin denaturation assays. The nanogel’s effectiveness was assessed by comparing its percentage inhibition values at different concentrations with those of standard antioxidants (ascorbic acid) and anti-inflammatory agents (diclofenac sodium).
Results:
The vitamin E-infused nanogel demonstrated antioxidant and anti-inflammatory properties comparable to standard drugs, with the highest activity observed at 100 µg/mL in both assays.
Conclusion:
The study demonstrates that the vitamin E-infused nanogel exhibits promising in-vitro antioxidant and anti-inflammatory activities, with inhibition percentages comparable to standard drugs. Further validation through statistical analysis and in-vivo studies is required to confirm its therapeutic potential in managing oral potentially malignant disorders. |
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| ISSN: | 0972-1363 0975-1572 |