Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization
This report presents the application of ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with positive electrospray ionization, to determine ramipril in human plasma. First, the proteins in human plasma were precipitated using acetonitrile, then the supe...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2015-0018 |
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| author | Szpot Paweł Buszewicz Grzegorz |
| author_facet | Szpot Paweł Buszewicz Grzegorz |
| author_sort | Szpot Paweł |
| collection | DOAJ |
| description | This report presents the application of ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with positive electrospray ionization, to determine ramipril in human plasma. First, the proteins in human plasma were precipitated using acetonitrile, then the supernatant was extracted by ethyl acetate at pH 3 and finally, the extract was analyzed using a UPLC-QTOF- MS system. The method was validated and the coefficient of determination (R2) was > 0.999, the lower limit of quantification (LLOQ) was 0.5 ng mL-1. Precision, recovery and stability were determined for three different concentrations of ramipril. RSD for this method ranged from 3.3 to 8.6 %. The intra-day mean recovery was from 65.3 to 97.3 %. In addition, the fragmentation of ramipril was studied. Due to high resolution of the spectrometer, it was possible to measure fragment masses accurately and determine their molecular and chemical formulas with high accuracy. |
| format | Article |
| id | doaj-art-4dc554e22abd48b190877dd8d73cf472 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-4dc554e22abd48b190877dd8d73cf4722025-02-02T09:44:14ZengSciendoActa Pharmaceutica1846-95582015-06-0165215916910.1515/acph-2015-0018acph-2015-0018Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionizationSzpot Paweł0Buszewicz Grzegorz1Laboratory of Forensic Toxicology Medical University of Lublin 20-090 Lublin, PolandLaboratory of Forensic Toxicology Medical University of Lublin 20-090 Lublin, PolandThis report presents the application of ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with positive electrospray ionization, to determine ramipril in human plasma. First, the proteins in human plasma were precipitated using acetonitrile, then the supernatant was extracted by ethyl acetate at pH 3 and finally, the extract was analyzed using a UPLC-QTOF- MS system. The method was validated and the coefficient of determination (R2) was > 0.999, the lower limit of quantification (LLOQ) was 0.5 ng mL-1. Precision, recovery and stability were determined for three different concentrations of ramipril. RSD for this method ranged from 3.3 to 8.6 %. The intra-day mean recovery was from 65.3 to 97.3 %. In addition, the fragmentation of ramipril was studied. Due to high resolution of the spectrometer, it was possible to measure fragment masses accurately and determine their molecular and chemical formulas with high accuracy.https://doi.org/10.1515/acph-2015-0018ramipriluplc-q-tof-msfragmentation pathway |
| spellingShingle | Szpot Paweł Buszewicz Grzegorz Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization Acta Pharmaceutica ramipril uplc-q-tof-ms fragmentation pathway |
| title | Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization |
| title_full | Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization |
| title_fullStr | Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization |
| title_full_unstemmed | Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization |
| title_short | Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization |
| title_sort | determination of ramipril in human plasma and its fragmentation by uplc q tof ms with positive electrospray ionization |
| topic | ramipril uplc-q-tof-ms fragmentation pathway |
| url | https://doi.org/10.1515/acph-2015-0018 |
| work_keys_str_mv | AT szpotpaweł determinationoframiprilinhumanplasmaanditsfragmentationbyuplcqtofmswithpositiveelectrosprayionization AT buszewiczgrzegorz determinationoframiprilinhumanplasmaanditsfragmentationbyuplcqtofmswithpositiveelectrosprayionization |