Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers
The activity of biocatalysts in nonaqueous solvents is related to the interaction of organic solvents with cells or enzymes. The behavior of proteins is strongly dependent on the protonation state of their ionizable groups, which ionization constants are greatly affected by the solvent. Due to the w...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/697161 |
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| author | Guangnan Ou Biyan He Xuejing Li Jianhui Lei |
| author_facet | Guangnan Ou Biyan He Xuejing Li Jianhui Lei |
| author_sort | Guangnan Ou |
| collection | DOAJ |
| description | The activity of biocatalysts in nonaqueous solvents is related to the interaction of organic solvents with cells or enzymes. The behavior of proteins is strongly dependent on the protonation state of their ionizable groups, which ionization constants are greatly affected by the solvent. Due to the weak ionizing and dissociating powers of common organic solvents, the charge of the protein will change significantly when the protein is transferred from water to common organic solvents, resulting in protein denaturation. In this work, glycerol carbonate (GC) was synthesized, which ionizing and dissociating abilities were very close to those of water. Transesterification activities of Candida antarctica lipase B (CALB) in GC were comparable to those in water and remained constant during 4-week storage. Bacillus subtilis and Saccharomyecs cerevisiae were cultured in liquid media containing GC with test tubes. In the medium containing low GC concentration, Bacillus subtilis and Saccharomyecs cerevisiae grew well as in a medium containing no organic solvent, but, in the medium containing high GC concentration, the growth of Bacillus subtilis and Saccharomyecs cerevisiae was suppressed. The results suggested that GC is a potential biosolvent, which has great significance to biocatalysis in nonaqueous solvents. |
| format | Article |
| id | doaj-art-4dc09f0ea4bf47a9aa793e6aab5d0093 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4dc09f0ea4bf47a9aa793e6aab5d00932025-02-03T01:32:50ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/697161697161Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating PowersGuangnan Ou0Biyan He1Xuejing Li2Jianhui Lei3School of Bioengineering, Jimei University, Xiamen 361021, ChinaSchool of Bioengineering, Jimei University, Xiamen 361021, ChinaSchool of Bioengineering, Jimei University, Xiamen 361021, ChinaSchool of Bioengineering, Jimei University, Xiamen 361021, ChinaThe activity of biocatalysts in nonaqueous solvents is related to the interaction of organic solvents with cells or enzymes. The behavior of proteins is strongly dependent on the protonation state of their ionizable groups, which ionization constants are greatly affected by the solvent. Due to the weak ionizing and dissociating powers of common organic solvents, the charge of the protein will change significantly when the protein is transferred from water to common organic solvents, resulting in protein denaturation. In this work, glycerol carbonate (GC) was synthesized, which ionizing and dissociating abilities were very close to those of water. Transesterification activities of Candida antarctica lipase B (CALB) in GC were comparable to those in water and remained constant during 4-week storage. Bacillus subtilis and Saccharomyecs cerevisiae were cultured in liquid media containing GC with test tubes. In the medium containing low GC concentration, Bacillus subtilis and Saccharomyecs cerevisiae grew well as in a medium containing no organic solvent, but, in the medium containing high GC concentration, the growth of Bacillus subtilis and Saccharomyecs cerevisiae was suppressed. The results suggested that GC is a potential biosolvent, which has great significance to biocatalysis in nonaqueous solvents.http://dx.doi.org/10.1100/2012/697161 |
| spellingShingle | Guangnan Ou Biyan He Xuejing Li Jianhui Lei Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers The Scientific World Journal |
| title | Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers |
| title_full | Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers |
| title_fullStr | Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers |
| title_full_unstemmed | Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers |
| title_short | Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers |
| title_sort | glycerol carbonate a novel biosolvent with strong ionizing and dissociating powers |
| url | http://dx.doi.org/10.1100/2012/697161 |
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