Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.)
Secondary metabolites fingerprint screening procedure showed, that potato cultivars ’Saskia’ and ‘Santé’ significantly differed in their respond to PVYNTN virus inoculation/infection of plant material. A typical chemical profile (with characteristic "virus peaks") was observed in virus -...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-09-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/16041 |
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| author | Dea BARIČEVIČ Breda SIMONOVSKA Adolf KRAŠNA Alenka ZUPANČIČ |
| author_facet | Dea BARIČEVIČ Breda SIMONOVSKA Adolf KRAŠNA Alenka ZUPANČIČ |
| author_sort | Dea BARIČEVIČ |
| collection | DOAJ |
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Secondary metabolites fingerprint screening procedure showed, that potato cultivars ’Saskia’ and ‘Santé’ significantly differed in their respond to PVYNTN virus inoculation/infection of plant material. A typical chemical profile (with characteristic "virus peaks") was observed in virus - sensitive ’Saskia’ but not in virus tolerant ’Santé’ plant extracts. Low temperature stress (4 °C) caused a reduction in growth of both cultivars and typical "low temperature" chromatograms with polar aromatic secondary metabolites. Exogenous abscisic acid (ABA), when applied at low temperature, in both cultivars influenced a further growth decrease, but diminished "low temperature signals" of fingerprint profile. When applied to control plants at 18 °C, ABA lost its growth inhibitory effect, although fingerprint "ABA signal" was clearly visible. Results of TLC semiquantitive determination of potato glycoalkaloids show, that a-chaconin may vary depending on temperature stress or ABA spraying of plant material. Cv. ’Saskia’ responds to low temperature with significantly raise of a-chaconin content in plant tissue (32.0 mg/100g fresh weight, fw) when compared with samples grown at normal temperature (17.0 mg/100g fw). PVYNTN infection did not affect the contents of a-chaconin (19.0 mg/100 g fw) in leaves of cv.’Saskia’, when compared with noninfected samples (17.0 mg/100 g fw). The content of a-solanin in leaves extracts was generally low (in case of virus infected material under the detection limit of 7.5 mg/100g fw). Results of the study indicate that, from a biochemical point of view, infection with PVYNTN virus can be considered as a specific stress reaction of virus - sensitive cultivars.
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| format | Article |
| id | doaj-art-4db239e5fc8b4e5d902e4d40abb6dc59 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 1998-09-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-4db239e5fc8b4e5d902e4d40abb6dc592025-08-20T02:59:11ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-09-0171110.14720/aas.1998.71.1.1604122434Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.)Dea BARIČEVIČ0Breda SIMONOVSKA1Adolf KRAŠNA2Alenka ZUPANČIČ3University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia Secondary metabolites fingerprint screening procedure showed, that potato cultivars ’Saskia’ and ‘Santé’ significantly differed in their respond to PVYNTN virus inoculation/infection of plant material. A typical chemical profile (with characteristic "virus peaks") was observed in virus - sensitive ’Saskia’ but not in virus tolerant ’Santé’ plant extracts. Low temperature stress (4 °C) caused a reduction in growth of both cultivars and typical "low temperature" chromatograms with polar aromatic secondary metabolites. Exogenous abscisic acid (ABA), when applied at low temperature, in both cultivars influenced a further growth decrease, but diminished "low temperature signals" of fingerprint profile. When applied to control plants at 18 °C, ABA lost its growth inhibitory effect, although fingerprint "ABA signal" was clearly visible. Results of TLC semiquantitive determination of potato glycoalkaloids show, that a-chaconin may vary depending on temperature stress or ABA spraying of plant material. Cv. ’Saskia’ responds to low temperature with significantly raise of a-chaconin content in plant tissue (32.0 mg/100g fresh weight, fw) when compared with samples grown at normal temperature (17.0 mg/100g fw). PVYNTN infection did not affect the contents of a-chaconin (19.0 mg/100 g fw) in leaves of cv.’Saskia’, when compared with noninfected samples (17.0 mg/100 g fw). The content of a-solanin in leaves extracts was generally low (in case of virus infected material under the detection limit of 7.5 mg/100g fw). Results of the study indicate that, from a biochemical point of view, infection with PVYNTN virus can be considered as a specific stress reaction of virus - sensitive cultivars. https://journals.uni-lj.si/aas/article/view/16041potatovirusaromatic secondary metabolitesglycoalkaloidsPVYNTNstress |
| spellingShingle | Dea BARIČEVIČ Breda SIMONOVSKA Adolf KRAŠNA Alenka ZUPANČIČ Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) Acta Agriculturae Slovenica potato virus aromatic secondary metabolites glycoalkaloids PVYNTN stress |
| title | Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) |
| title_full | Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) |
| title_fullStr | Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) |
| title_full_unstemmed | Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) |
| title_short | Screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn - infected potato cultivars (Solanum tuberosum L.) |
| title_sort | screening of aromatic secondary metabolites and glycoalcaloids in two pvyntn infected potato cultivars solanum tuberosum l |
| topic | potato virus aromatic secondary metabolites glycoalkaloids PVYNTN stress |
| url | https://journals.uni-lj.si/aas/article/view/16041 |
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