RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
Abstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in...
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83738-4 |
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| author | Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin |
| author_facet | Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin |
| author_sort | Heekyoung Kang |
| collection | DOAJ |
| description | Abstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in this study to establish in vivo AK model. A. castellanii cell suspensions (equal mixtures of trophozoites and cysts) were loaded onto 2-mm contact lens pieces and inserted into mouse eyes that were scratched using an ophthalmic surgical blade under anesthesia and the eyelids of the mice were sutured. The AK signs were grossly observed and PCR was performed using P-FLA primers to amplify the Acanthamoeba 18S-rRNA gene from mouse ocular tissue. The experimental AK mouse model was characterized by typical hazy blurring and melting of the mouse cornea established on day 1 post-inoculation. AK was induced with at least 0.3 × 105 A. castellanii cells (optimal number, 5 × 104), and the infection persisted for two months. The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods. In conclusion, the present AK mouse model may serve as an important in vivo model for the development of various therapeutic drugs against AK. |
| format | Article |
| id | doaj-art-4dab0f660cf246e39d62d4cfaaedcfde |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4dab0f660cf246e39d62d4cfaaedcfde2025-02-02T12:25:44ZengNature PortfolioScientific Reports2045-23222021-02-0111111010.1038/s41598-021-83738-4RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplificationHeekyoung Kang0Hae-Jin Sohn1A-Young Park2A-Jeong Ham3Jeong-Heon Lee4Young-Hwan Oh5Yong-Joon Chwae6Kyongmin Kim7Sun Park8Hongseok Yang9Suk-Yul Jung10Jong-Hyun Kim11Ho-Joon Shin12Department of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineDepartment of Microbiology, Ajou University School of MedicineYonsei Eye ClinicDepartment of Biomedical Laboratory Science, Molecular Diagnostics Research Institute, School of Health and Medicine, Namseoul UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Microbiology, Ajou University School of MedicineAbstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in this study to establish in vivo AK model. A. castellanii cell suspensions (equal mixtures of trophozoites and cysts) were loaded onto 2-mm contact lens pieces and inserted into mouse eyes that were scratched using an ophthalmic surgical blade under anesthesia and the eyelids of the mice were sutured. The AK signs were grossly observed and PCR was performed using P-FLA primers to amplify the Acanthamoeba 18S-rRNA gene from mouse ocular tissue. The experimental AK mouse model was characterized by typical hazy blurring and melting of the mouse cornea established on day 1 post-inoculation. AK was induced with at least 0.3 × 105 A. castellanii cells (optimal number, 5 × 104), and the infection persisted for two months. The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods. In conclusion, the present AK mouse model may serve as an important in vivo model for the development of various therapeutic drugs against AK.https://doi.org/10.1038/s41598-021-83738-4 |
| spellingShingle | Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification Scientific Reports |
| title | RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification |
| title_full | RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification |
| title_fullStr | RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification |
| title_full_unstemmed | RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification |
| title_short | RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification |
| title_sort | retracted article establishment of an acanthamoeba keratitis mouse model confirmed by amoebic dna amplification |
| url | https://doi.org/10.1038/s41598-021-83738-4 |
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