Genome-Wide Analysis of DNA Methylation in Human Amnion
The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion fro...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/678156 |
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| author | Jinsil Kim Mitchell M. Pitlick Paul J. Christine Amanda R. Schaefer Cesar Saleme Belén Comas Viviana Cosentino Enrique Gadow Jeffrey C. Murray |
| author_facet | Jinsil Kim Mitchell M. Pitlick Paul J. Christine Amanda R. Schaefer Cesar Saleme Belén Comas Viviana Cosentino Enrique Gadow Jeffrey C. Murray |
| author_sort | Jinsil Kim |
| collection | DOAJ |
| description | The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. |
| format | Article |
| id | doaj-art-4da63e779a994d909ce8a773c9d85f07 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4da63e779a994d909ce8a773c9d85f072025-02-03T05:51:58ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/678156678156Genome-Wide Analysis of DNA Methylation in Human AmnionJinsil Kim0Mitchell M. Pitlick1Paul J. Christine2Amanda R. Schaefer3Cesar Saleme4Belén Comas5Viviana Cosentino6Enrique Gadow7Jeffrey C. Murray8Department of Anatomy and Cell Biology, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USADepartment of Pediatrics, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USADepartment of Pediatrics, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USADepartment of Pediatrics, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USADepartamento de Neonatología, Instituto de Maternidad y Ginecología Nuestra Señora de las Mercedes, 4000 San Miguel de Tucumán, ArgentinaDirección de Investigación, Centro de Educación Médica e Investigaciones Clínicas (CEMIC), 1431 Buenos Aires, ArgentinaDirección de Investigación, Centro de Educación Médica e Investigaciones Clínicas (CEMIC), 1431 Buenos Aires, ArgentinaDirección de Investigación, Centro de Educación Médica e Investigaciones Clínicas (CEMIC), 1431 Buenos Aires, ArgentinaDepartment of Anatomy and Cell Biology, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USAThe amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies.http://dx.doi.org/10.1155/2013/678156 |
| spellingShingle | Jinsil Kim Mitchell M. Pitlick Paul J. Christine Amanda R. Schaefer Cesar Saleme Belén Comas Viviana Cosentino Enrique Gadow Jeffrey C. Murray Genome-Wide Analysis of DNA Methylation in Human Amnion The Scientific World Journal |
| title | Genome-Wide Analysis of DNA Methylation in Human Amnion |
| title_full | Genome-Wide Analysis of DNA Methylation in Human Amnion |
| title_fullStr | Genome-Wide Analysis of DNA Methylation in Human Amnion |
| title_full_unstemmed | Genome-Wide Analysis of DNA Methylation in Human Amnion |
| title_short | Genome-Wide Analysis of DNA Methylation in Human Amnion |
| title_sort | genome wide analysis of dna methylation in human amnion |
| url | http://dx.doi.org/10.1155/2013/678156 |
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