The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species
The genome composition of intermediate wheatgrass (IWG; <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey; 2n = 6x = 42) is complex and remains to be a subject of ongoing investigation. This study employed fluorescence in situ hybridization (FISH) to analyze the karyotype of...
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2025-01-01
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| author | Lin Wang Shuang Liang Fei Qi Yinguang Bao Richard R.-C. Wang Xingfeng Li |
| author_facet | Lin Wang Shuang Liang Fei Qi Yinguang Bao Richard R.-C. Wang Xingfeng Li |
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| description | The genome composition of intermediate wheatgrass (IWG; <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey; 2n = 6x = 42) is complex and remains to be a subject of ongoing investigation. This study employed fluorescence in situ hybridization (FISH) to analyze the karyotype of <i>Th. intermedium</i> and its related species. With the St<sub>2</sub>-80 probe derived from <i>Pseudoroegneria strigosa</i> and the pDb12H probe from <i>Dasypyrum breviaristatum</i>, FISH analysis classified the chromosomes of <i>Th. intermedium</i> as <b>J<sup>vs</sup>J<sup>vs</sup>J<sup>r</sup>J<sup>r</sup>StSt</b>. FISH karyotype was established using pSc119.2-1, (GAA)<sub>10</sub>, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4, and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio, and karyotype structure. The karyotype formula of <i>Th. intermedium</i> is K(2n) = 6X = 42 = 36m + 6sm, and that of <i>Th. junceiforme</i> is K(2n) = 4X = 28 = 22m + 6sm. The karyotype formula of <i>Th. elongatum</i> and <i>Th. bessarabicum</i> is K(2n) = 2X = 14 = 12m + 2sm, of <i>Ps. spicata</i> is K(2n) = 2X = 14 = 2M + 12m, and of <i>Da. villosum</i> is K(2n) = 2X = 14 = 12m + 2sm. Based on the results of FISH, standard karyotypes of <i>Th. intermedium</i> and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of <i>Th. intermedium</i> was abundant and asymmetrical. |
| format | Article |
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| spelling | doaj-art-4d6e0fcf13894b27bf5c8e1f30281fa82025-01-24T13:46:42ZengMDPI AGPlants2223-77472025-01-0114219610.3390/plants14020196The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor SpeciesLin Wang0Shuang Liang1Fei Qi2Yinguang Bao3Richard R.-C. Wang4Xingfeng Li5State Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai’an 271018, ChinaUSDA-ARS, Forage & Range Research Laboratory (FRRL), Logan, UT 84322-6300, USAState Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai’an 271018, ChinaThe genome composition of intermediate wheatgrass (IWG; <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey; 2n = 6x = 42) is complex and remains to be a subject of ongoing investigation. This study employed fluorescence in situ hybridization (FISH) to analyze the karyotype of <i>Th. intermedium</i> and its related species. With the St<sub>2</sub>-80 probe derived from <i>Pseudoroegneria strigosa</i> and the pDb12H probe from <i>Dasypyrum breviaristatum</i>, FISH analysis classified the chromosomes of <i>Th. intermedium</i> as <b>J<sup>vs</sup>J<sup>vs</sup>J<sup>r</sup>J<sup>r</sup>StSt</b>. FISH karyotype was established using pSc119.2-1, (GAA)<sub>10</sub>, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4, and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio, and karyotype structure. The karyotype formula of <i>Th. intermedium</i> is K(2n) = 6X = 42 = 36m + 6sm, and that of <i>Th. junceiforme</i> is K(2n) = 4X = 28 = 22m + 6sm. The karyotype formula of <i>Th. elongatum</i> and <i>Th. bessarabicum</i> is K(2n) = 2X = 14 = 12m + 2sm, of <i>Ps. spicata</i> is K(2n) = 2X = 14 = 2M + 12m, and of <i>Da. villosum</i> is K(2n) = 2X = 14 = 12m + 2sm. Based on the results of FISH, standard karyotypes of <i>Th. intermedium</i> and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of <i>Th. intermedium</i> was abundant and asymmetrical.https://www.mdpi.com/2223-7747/14/2/196Triticeaekaryotype analysisgenome constitutionFISHevolution |
| spellingShingle | Lin Wang Shuang Liang Fei Qi Yinguang Bao Richard R.-C. Wang Xingfeng Li The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species Plants Triticeae karyotype analysis genome constitution FISH evolution |
| title | The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species |
| title_full | The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species |
| title_fullStr | The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species |
| title_full_unstemmed | The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species |
| title_short | The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species |
| title_sort | construction of a standard karyotype of intermediate wheatgrass and its potential progenitor species |
| topic | Triticeae karyotype analysis genome constitution FISH evolution |
| url | https://www.mdpi.com/2223-7747/14/2/196 |
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