Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’
In this study we investigate the capability of lesions, performed between embryonic day E18 and postnatal day P6, to provoke glial reaction. Two different lesion types were applied: ‘severe’ lesion (tissue defect) and ’light’ lesion (stab wound). The glial reaction was detected with immunostain[ng a...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2000.147 |
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| author | Mihály Kálmán Béla M. Ajtai Jon Håvard Sommernes |
| author_facet | Mihály Kálmán Béla M. Ajtai Jon Håvard Sommernes |
| author_sort | Mihály Kálmán |
| collection | DOAJ |
| description | In this study we investigate the capability of
lesions, performed between embryonic day E18
and postnatal day P6, to provoke glial reaction.
Two different lesion types were applied: ‘severe’
lesion (tissue defect) and ’light’ lesion (stab
wound). The glial reaction was detected with
immunostain[ng against glial fibrillary acidic
protein. When performed as early as P0, severe
lesions could result in reactive gliosis, which
persisted even after a month. The glial reaction
was detected at P6/P7 and became strong by P8,
regardless of the age when the animals were
lesioned between P0 and P5. Namely, a strict
limit could be estimated for the age when
reactive glia were already found rather than for
the age when glial reaction-provoking lesions
could occur. After prenatal lesions, no glial
reaction developed, but the usual glia limitans
covered the deformed brain, surface. Light
lesions provoked glial reactions when
performed at P6. In conclusion, three scenarios
were found, depending on the age of the animal
at injury: (i) healing without glial reaction,
regardless of the remaining deformation; (ii) depending on the size of the lesion, either healing without residuum or with remaining
tissue defect plus reactive gliosis; and (iii) healing always with reactive gliosis. The age limits between them were at P0 and P5. The glial reactivity seemingly appears after the end of the neuronal migration and just precedes the massive transformation of the radial glia into astrocytes. Estimating the position of the appearance of glial reactivity among the events of cortical maturation can help to adapt the experimental results to humans. |
| format | Article |
| id | doaj-art-4d30181fa6ab404681bdf44c6a853a62 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-4d30181fa6ab404681bdf44c6a853a622025-02-03T05:58:40ZengWileyNeural Plasticity2090-59041687-54432000-01-017314716510.1155/NP.2000.147Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’Mihály Kálmán0Béla M. Ajtai1Jon Håvard Sommernes2Dept. of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tûzoltó 58, Budapest H-1450, HungaryDept. of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tûzoltó 58, Budapest H-1450, HungaryDept. of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tûzoltó 58, Budapest H-1450, HungaryIn this study we investigate the capability of lesions, performed between embryonic day E18 and postnatal day P6, to provoke glial reaction. Two different lesion types were applied: ‘severe’ lesion (tissue defect) and ’light’ lesion (stab wound). The glial reaction was detected with immunostain[ng against glial fibrillary acidic protein. When performed as early as P0, severe lesions could result in reactive gliosis, which persisted even after a month. The glial reaction was detected at P6/P7 and became strong by P8, regardless of the age when the animals were lesioned between P0 and P5. Namely, a strict limit could be estimated for the age when reactive glia were already found rather than for the age when glial reaction-provoking lesions could occur. After prenatal lesions, no glial reaction developed, but the usual glia limitans covered the deformed brain, surface. Light lesions provoked glial reactions when performed at P6. In conclusion, three scenarios were found, depending on the age of the animal at injury: (i) healing without glial reaction, regardless of the remaining deformation; (ii) depending on the size of the lesion, either healing without residuum or with remaining tissue defect plus reactive gliosis; and (iii) healing always with reactive gliosis. The age limits between them were at P0 and P5. The glial reactivity seemingly appears after the end of the neuronal migration and just precedes the massive transformation of the radial glia into astrocytes. Estimating the position of the appearance of glial reactivity among the events of cortical maturation can help to adapt the experimental results to humans.http://dx.doi.org/10.1155/NP.2000.147 |
| spellingShingle | Mihály Kálmán Béla M. Ajtai Jon Håvard Sommernes Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ Neural Plasticity |
| title | Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ |
| title_full | Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ |
| title_fullStr | Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ |
| title_full_unstemmed | Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ |
| title_short | Characteristics of Glial Reaction in the Perinatal Rat Cortex: Effect of Lesion Size in the ‘Critical Period’ |
| title_sort | characteristics of glial reaction in the perinatal rat cortex effect of lesion size in the critical period |
| url | http://dx.doi.org/10.1155/NP.2000.147 |
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