Differential diagnosis of fasciola hepatica from different animal hosts by pcr
<b>Aim:</b> The objective of this study was evaluated differentiational diagnosis of Fasciola hepatica from sheep and goat by PCR.<p> <b>Materials and Methods:</b> F. hepatica were obtained from sheep and goat. These parasites were washed several times with pH:7.4 phosp...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1036 |
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| author | Osman Selcuk Aldemir Mederle Narcisa |
| author_facet | Osman Selcuk Aldemir Mederle Narcisa |
| author_sort | Osman Selcuk Aldemir |
| collection | DOAJ |
| description | <b>Aim:</b> The objective of this study was evaluated differentiational
diagnosis of Fasciola hepatica from sheep and goat by
PCR.<p>
<b>Materials and Methods:</b> F. hepatica were obtained from
sheep and goat. These parasites were washed several times
with pH:7.4 phosphate-buffered saline and phenol extraction/
ethanol precipitation method was employed in genomic
DNA extraction. In order to optimization of the PCR conditions,
five primers were selected to amplify the DNA of each
F. hepatica.<p>
<b>Results:</b> Both sheep and goat were amplified products in
different numbers and sizes by RAPD-PCR technique. The
results show that different primers gave different bands
(fragments) and allowed to identify genetic variations of F.
hepatica. These bands were species-specific to F. hepatica
from goat and sheep.<p>
<b>Conclusions:</b> The RAPD-PCR method can be useful for the
differentiational diagnosis of F. hepatica of sheep and goat. |
| format | Article |
| id | doaj-art-4d11ec9b358a4c67873f310b3aa7f656 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-4d11ec9b358a4c67873f310b3aa7f6562025-02-03T11:25:40ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195330285881036Differential diagnosis of fasciola hepatica from different animal hosts by pcrOsman Selcuk Aldemir0Mederle Narcisa1Department of Parasitology, Faculty of Veterinary Medicine, Adnan Menderes University, Isıklı, Aydin, TurkeyUniversitatea de StiinNe Agricole si Medicina Veterinara a Banatului Timisoara, Facultatea de Medicina Veterinara, Timisoara, Romania<b>Aim:</b> The objective of this study was evaluated differentiational diagnosis of Fasciola hepatica from sheep and goat by PCR.<p> <b>Materials and Methods:</b> F. hepatica were obtained from sheep and goat. These parasites were washed several times with pH:7.4 phosphate-buffered saline and phenol extraction/ ethanol precipitation method was employed in genomic DNA extraction. In order to optimization of the PCR conditions, five primers were selected to amplify the DNA of each F. hepatica.<p> <b>Results:</b> Both sheep and goat were amplified products in different numbers and sizes by RAPD-PCR technique. The results show that different primers gave different bands (fragments) and allowed to identify genetic variations of F. hepatica. These bands were species-specific to F. hepatica from goat and sheep.<p> <b>Conclusions:</b> The RAPD-PCR method can be useful for the differentiational diagnosis of F. hepatica of sheep and goat.http://eurasianjvetsci.org/pdf.php3?id=1036differentiational diagnosisfasciola hepaticarapd-pcrsheepgoat |
| spellingShingle | Osman Selcuk Aldemir Mederle Narcisa Differential diagnosis of fasciola hepatica from different animal hosts by pcr Eurasian Journal of Veterinary Sciences differentiational diagnosis fasciola hepatica rapd-pcr sheep goat |
| title | Differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| title_full | Differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| title_fullStr | Differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| title_full_unstemmed | Differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| title_short | Differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| title_sort | differential diagnosis of fasciola hepatica from different animal hosts by pcr |
| topic | differentiational diagnosis fasciola hepatica rapd-pcr sheep goat |
| url | http://eurasianjvetsci.org/pdf.php3?id=1036 |
| work_keys_str_mv | AT osmanselcukaldemir differentialdiagnosisoffasciolahepaticafromdifferentanimalhostsbypcr AT mederlenarcisa differentialdiagnosisoffasciolahepaticafromdifferentanimalhostsbypcr |