Differential diagnosis of fasciola hepatica from different animal hosts by pcr
<b>Aim:</b> The objective of this study was evaluated differentiational diagnosis of Fasciola hepatica from sheep and goat by PCR.<p> <b>Materials and Methods:</b> F. hepatica were obtained from sheep and goat. These parasites were washed several times with pH:7.4 phosp...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1036 |
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| Summary: | <b>Aim:</b> The objective of this study was evaluated differentiational
diagnosis of Fasciola hepatica from sheep and goat by
PCR.<p>
<b>Materials and Methods:</b> F. hepatica were obtained from
sheep and goat. These parasites were washed several times
with pH:7.4 phosphate-buffered saline and phenol extraction/
ethanol precipitation method was employed in genomic
DNA extraction. In order to optimization of the PCR conditions,
five primers were selected to amplify the DNA of each
F. hepatica.<p>
<b>Results:</b> Both sheep and goat were amplified products in
different numbers and sizes by RAPD-PCR technique. The
results show that different primers gave different bands
(fragments) and allowed to identify genetic variations of F.
hepatica. These bands were species-specific to F. hepatica
from goat and sheep.<p>
<b>Conclusions:</b> The RAPD-PCR method can be useful for the
differentiational diagnosis of F. hepatica of sheep and goat. |
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| ISSN: | 1309-6958 2146-1953 |